National Journals

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Start date: 2020End date: 2023

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Now showing 1 - 10 of 21
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    TANNERY WASTEWATER REMEDIATION COMPETENCE OF METAL TOLERANT BACTERIA AND FUNGI UNDER THE INFLUENCE OF CHEMICALLY MODIFIED WATER HYACINTH BIOCHAR: AN IN VITRO EVALUATION
    (Springer Link, 2023-11-22) Kandasamy, Gajendiran; Ying, Ma; Sabariswaran, Kandasamy; Amal Abdullah, A. Sabour; Maha, Alshiekheid; Arivalagan, Pugazhendhi; Mathiyazhagan, Narayanan
    This study was trying to find a sustainable approach to remediate the tannery wastewater by various treatments sets (set-I to set-VIII: consists of KOH modified biochar, Bacillus cereus, and Aspergillus flavus biomass), and treatment successfulness was determined by phytotoxicity and cytotoxicity studies on potential Vigna unguiculata seedlings and Artemia franciscana larvae, respectively. Three tannery wastewater samples were collected from 3 sites (I, II, and III); among them, the physicochemical properties of site I were beyond the permissible limits containing more volume of harmful heavy metals such as Cr, Cd, Pb, As, Co, Cu, Fe, Zn, and Mn than other sample. The test B. cereus and A. flavus showed remarkable metal tolerance to heavy metals such as Cr, Cd, Pb, As, Co, Cu, Fe, Zn, and Mn at 800 µg mL−1. The bioremediation study results stated that the KOH modified pre-synthesized and characterized water hyacinth biochar with the blend of B. cereus and A. flavus (treatment set-VII) biomass substantially reduced/removed (Cr, Cd, Pd, As, Co, Cu, Fe, Zn, and Mn as 54.75%, 49.52%, 30.49%, 17.53%, 29.07%, 14.75%, 5%, 27.27%, and 9.2%, respectively) the heavy metals from the tannery wastewater. The treatment effectiveness was determined by phytotoxicity and cytotoxicity studies on V. unguiculata seedlings and A. franciscana larvae, respectively. Among various treatment sets, the set-VII demonstrated absence of phytotoxicity and cytotoxicity on V. unguiculata seedlings and A. franciscana larvae, respectively. This clearly shows that the KOH modified biochar with B. cereus and A. flavus biomass can be used to manage and treat tannery wastewater in a sustainable manner.
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    DEVELOPMENT OF PORTABLE COLORIMETER FOR ON-SITE DETERMINATION OF WATER QUALITY IN AQUACULTURE
    (Pigment & Resin Technology, 2022-05-25) Kesavan, Devarayan; Madhan, Kathavarayan; Anand, Theivasigamani; Monikandon, Sukumaran; Sabariswaran, Kandasamy
    This study aims to describe the development of a battery-operated portable colorimeter for on-site determination of water quality in aquaculture.A simple and economical colorimeter is built using light sources of different wavelength and a light-dependent resistor combined with an electrical circuit. The whole system was fabricated as to fit into the pocket or palm for easy handling. The developed portable colorimeter was calibrated for estimation of nitrite. Further, the performance of developed portable colorimeters was compared with the commercially available colorimeter.Three colorimeters with different light sources were developed and calibrated for determination of nitrite in water. Among them, colorimeter with yellow light source exhibited higher potential for determination of nitrite in the range of 0.5–3.5 ppm. Further, the results of the developed colorimeter are comparable with the commercial colorimeter.The portable colorimeter developed in this study exhibits potential for determination of nitrite in aquaculture. Determination of nitrite at low concentrations is important for assessing the quality of culture as well as wastewater in aquaculture industry. The accuracy, portable nature, economy and simple operation of these portable colorimeters offer opportunity for on-site determination of water quality parameters in aquaculture.
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    A REVIEW ON HYDROTHERMAL LIQUEFACTION OF ALGAL BIOMASS ON PROCESS PARAMETERS, PURIFICATION AND APPLICATIONS
    (Elsevier, 2022-04-01) Sathish Raam, Ravichandran; Chitra Devi, Venkatachalam; Mothil, Sengottian; Sarath, Sekar; Sabariswaran, Kandasamy; Kesav Prasath, Ramasamy Subramanian; Kirubakaran, Purushothaman; Aravindan Lavanya, Chandrasekaran; Mathiyazhagan, Narayanan
    Algae, a potential biomass feedstock with a faster growth rate and capability of greenhouse gas absorption, mitigates the limitations of the first- and second-generation feedstock in bio-oil production. hydrothermal liquefaction (HTL) is known to be an active method capable of producing substantial energy resources. In HTL, biomass undergoes thermal depolymerization in the presence of water, at around 280 °C–350 °C following subcritical and near supercritical conditions to produce chemical compounds such as alkanes, nitrogenates, esters, phenolics, etc. The primary product, “Biocrude/Bio-oil” obtained from the reaction, is identified as the essential fuel source after processing and also as a distinct value-added chemical source, along with biochar and biogas as co-products. This review outlines a range of routes available for thermochemical conversion of the algal biomass. It also provides a better understanding of the reaction mechanism like depolymerization, decomposition, and re-polymerization, operating conditions like temperature, pressure, the quantity of catalyst required, and the solvent used in the process. The review also highlights the yield achieved by altering the aforementioned parameters, comparing and presenting them as a collective result.
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    ANTIBIOFILM ACTIVITY OF BIOSURFACTANT PRODUCED BY A SPONGE-ASSOCIATED MARINE COBETIA SP. JCG-23
    (Springer Link, 2023-09-22) Govindarajan, Ganesan; Chandrasekar, Balu; Suganthi, Ganesan; Samuel Raj, Babu Arulmani; Sabariswaran, Kandasamy
    Marine symbiotically associated microbes play a vital role and are an excellent source of natural compounds that exert wide biological activities. In this study, we have reported on the identification, characterization, phylogenetic relationship, and anti-biofilm surface-active compound-producing abilities of marine invertebrate sponge-associated Cobetia sp. JCG-23. Among 24 isolates, a total of five strains (JCG2, JCG19, JCG20, JCG22, and JCG23) have active surface molecule producing potential on the emulsification index assay. Interestingly, the potential candidate JCG-23, produces biosurfactants with low surface tension (22 Nm-1) that exert anti-biofilm activity against Pseudomonas aeruginosa PAO1. The isolate was identified as genus Cobetia sp. JCG-23 with 99.1% sequence similarity to Cobetia crustatorum (EU909460) based on 16S rRNA gene sequence analysis. The large-scale production, purification, stability, and characterization of biosurfactant were carried out and its surface activity was determined using the oil drop method. Subsequent spectral analysis such as UV, FT-IR, and GC-MS analysis indicated that the purified biosurfactant was a hydroxyl fatty acid, namely octadecanoic acid (C18H36O2) with a molecular weight of 284 m/z. Furthermore, the effect of antibiofilm activity on the viability of Pseudomonas aeruginosa PAO1 by static ring tube and light and confocal laser scanning microscopy analysis revealed that the octadecanoic acid from Cobetia sp. JCG-23 has strong biofilm dismantle ability against Pseudomonas aeruginosa PAO1. Further characterization of the biosurfactant from the isolate Cobetia sp. JCG-23 can pave the way for developing novel bioactive agents targeting biofilm-forming pathogens on topical and medical devices.
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    INFLUENCE OF GADOLINIUM DOPED IN NICKEL NANOFERRITES ON STRUCTURAL, OPTICAL, ELECTRICAL, AND MAGNETIC PROPERTIES
    (Elsevier, 2023-02) Bhuvaneshwari V; Lenin N; Shiva C; Kathirvel M; Ragavendran V; Rajeshwari A; Guruprakash B; Vinoth Kumar G.G
    The sonochemical reaction approach was used to make NiGdxFe2-xO4 nanoferrites (x = 0.01, 0.03, 0.05, 0.07, and 0.09). X-ray diffraction (XRD), ultra violet-diffuse reflectance spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, vibrating sample magnetometer, and electrochemical impedance spectroscopy were used to investigate the optical, magnetic, electrical, and structural properties of NiGdxFe2-xO4 nanoferrites. The creation of a cubic spinel structure was confirmed by analyzing the XRD pattern of these NiGdxFe2-xO4 nanoferrites. The average crystallite diameters of the nanoparticles for the compositions x = 0.01, 0.03, 0.05, 0.07, and 0.09 were 57, 53, 48, 45, and 41 nm, respectively. To investigate the dielectric behavior of the produced nanoferrites, an impedance study was performed. The addition of Gd to NiFe2-xO4 nanoparticles increased the dielectric characteristics of the produced nanoferrites, according to characterization experiments. A cation distribution has been proposed for the determination of various important theoretical parameters for these samples. The addition of Gd3+ nanoparticles has shown the ferromagnetic behavior at room temperature confirmed by VSM analysis. A specific correlation between magnetic interaction and lattice strain was observed in Gd3+ substituted nickel ferrite. An increase in Gd concentration in the manufactured nanoferrites resulted in a rise in saturation magnetization and a decrease in coercivity.
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    INSIGHTS FROM THE MOLECULAR DOCKING ANALYSIS OF COLISTIN WITH THE PMRA PROTEIN MODEL FROM ACINETOBACTER BAUMANNII
    (Pub Med, 2022) Shalini, Ganeshan; Mohammad, Reza Shakibaie; Rajaguru, Rajagopal
    Acinetobacter baumannii (AB) is one of the most common causes of nosocomial infections. Therefore, it is of interest to design and develop drugs against Acinetobacter baumannii. A strain of AB showing MIC 32 µg/ml against colistin was isolated from a hospital environment in Iran. Hence, we document data to glean insights from the molecular docking analysis of colistin with the PmrA protein from this bacterium.
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    A SCIENTIFIC PHARMACOGNOSY ON GAUCHER’S DISEASE: AN IN SILICO ANALYSIS
    (Springer Science and Business Media, 2022-04) Sasikumar, A P; Ramaswamy, S K; Sudhir, S
    From ancient times, studies on herbal medicine and pharmacognosy have increased gradually worldwide, due to the increased side effects, adverse drug reactions, and charge lines of modern medicines. Plants are well known for their medicinal effects and nutritional values. They contain bioactive compounds which display a wide spectrum of therapeutic effects. Gaucher’s disease (GD) is a rare autosomal recessively inherited metabolic disorder caused due to the defect in Glucosylceramidase beta gene coding for the enzyme acid-β-glucosidase in humans. We revealed the profound binding efficiency of five selected bioactive compounds from different plants against the main enzyme acid-β-glucosidase responsible for GD through molecular docking. An in silico approach along with the ADMET profiles of phytocompounds was done using the Schrodinger software. The preventive measure of GD leads to side effects, inaccessible and unaffordable which put forth the emergence of phytocompounds which have fewer toxic effects, and one such compound is β-D-Glucopyranose with the best docking score (–10.28 kcal/mol) and an excellent binding affinity than other ligands, which could be further analyzed for stability using molecular dynamics study and in vitro. Being a dietary supplement, these compounds could be prepared in any form of formulation as a drug.
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    BIOSYNTHESIS AND BIODEGRADATION OF POLY(3-HYDROXYBUTYRATE) FROM PRIESTIA FLEXA; A PROMISING MANGROVE HALOPHYTE TOWARDS THE DEVELOPMENT OF SUSTAINABLE ECO-FRIENDLY BIOPLASTICS
    (Elsevier, 2023-02) Nayana, Chathalingath; Joshua, Stephen Kingsly; Anbarasi, Gunasekar
    The protracted persistence of petrochemical plastics in the environment and their non-biodegradability impede the survival of living creatures. Recently, biopolymers are being thoroughly researched as a potential replacement for conventional plastics. This present study sought to locate Poly(3-hydroxybutyrate) synthesizing bacterial species prevalent in the mangrove ecosystem. Six halophilic bacterial isolates were obtained from the mangrove habitat, four isolates displayed superior cell dry weight as well as PHB accumulation. Isolate PMPHB5 showed the highest cell dry weight (4.92 ± 0.02 g/L), while the maximum PHA yield (80%) was found with PMPHB7. Hence, PMPHB7 was chosen for further optimization of carbon source wherein glucose demonstrated improved cell growth as well as PHB production. The characterization of the PHB granules was performed by FT-IR spectroscopy and FE-SEM EDX. The presence of characteristic elements in the sample was confirmed using EDX. Isolate PMPHB7 was further identified as Priestia flexa through 16S rRNA gene sequencing (GenBank accession number: ON362236) and a phylogenetic tree was constructed to reveal the molecular relationships of this organism with others. The solvent-cast biopolymer film was made to check the biodegradability of the extracted PHB. When buried in soil, it was found that the biopolymer film exhibited approximately 73% biodegradation after 21 days. Thus, the present study sheds light on the potential of mangrove-associated halophytes to efficiently produce PHB that is readily biodegradable in soil.
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    ELUCIDATING THE PHYSIOLOGICAL AND MOLECULAR CHARACTERISTICS OF BACTERIAL BLIGHT INCITANT XANTHOMONAS AUXONOPODIS PV. PUNICAE; A LIFE THREATENING PHYTOPATHOGEN OF POMEGRANATE (PUNICA GRANATUM. L) AND ASSESSMENT OF H2O2 ACCUMULATION DURING HOST-PATHOGEN INTERACTION
    (Pub Med, 2023-07-28) Nayana, Chathalingath; Anbarasi, Gunasekar
    Bacterial blight of pomegranate caused by Xanthomonas auxonopodis pv.punicae (Xap) threaten the existence of a group of farmers for the past few decades who rely on pomegranate cultivation for their livelihood since it will cause huge yield loss. The primary focus of this study was to conduct a thorough analysis of the characterization of this blight incitant Xap. Physiological, biochemical, and molecular characteristics of six phytopathogenic strains of Xap, designated as PBF1 (PBF: Pomegranate Blight Fruit), PBF2, PBF3, PBF4, PBF5, and PBF6, isolated from the infected fruits were examined. Bacterial colonies were featured as gram-negative, yellow-pigmented circular with a glistening appearance. An attempt to determine the best culture medium, favouring bacterial proliferation was successfully done with four distinct medium, Nutrient Glucose Agar (NGA), Nutrient sucrose Agar (NSA), Yeast Dextrose Calcium Carbonate Agar (YDCA) and Yeast Glucose Calcium Carbonate Agar (YGCA) and comparatively, significant growth was found in NGA (66.66%) followed by YDCA (33%). According to the antibiotic susceptibility results, both ampicillin and streptomycin were determined as potentially effective drugs in preventing the proliferation of Xap (P 0.05). The reactive oxygen species-mediated plant immune response during host-pathogen interaction was confirmed by accessing the presence of H2O2 accumulation in infected leaves via 3,3 - diaminobenzidine (DAB) -staining technique. Bacterial isolates from this study were confirmed by two universal constitutive genes such as gyrB and 16S rRNA. From the BLAST analysis, the isolates were identified as Xap with base pair lengths of 1408bp, 1180bp, and 1159bp, which correspond to PBF1, PBF2, and PBF3, respectively. A neighbor-joining phylogenetic tree study explaining a strong phylogenetic relationship between the query sequence and closely related bacterial species.
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    EFFICACY OF EISENIA BICYCLIS PHLOROTANNINS IN THE TREATMENT OF DIABETES AND REDUCING INFLAMMATION
    (Elsevier, 2023-04) Rathinam, Raja; Shanmugam, Hemaiswarya; Kulanthaiyesu, Arunkumar; N, Mathiyazhagan; Sabariswaran, Kandasamy; A, Arun; P, Ramasamy
    Crude phlorotannins from Eisenia bicyclis (CPEb), a common perennial brown seaweed were screened for pharmacological activities such as anti-inflammatory, antioxidant, and antidiabetic effects. Crude Phlorotannins (CPEb) studied by FT-IR, LC-MS, UHPLC, and UV chromatography showed the occurrence of dieckol, 8, 8'-bieckol, fucofuroeckol, and the carotenoid, zeaxanthin (0.5 mg/kg). LPS, an inflammatory mediator, enhanced the production of cytokines such IL-1 (interleukin-1), IL-6 (interleukin-6), IL-8 (interleukin-8), IL-10 (interleukin-10), TNF-α (tumour necrosis factor-α), chemokine CXCL10, and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) in differentiated THP1 macrophage cells. On differentiated human monocytic cell line LPS-induced THP1 cells, CPEb was found to have anti-inflammatory effects by reducing the expression of IL-1, IL-6, IL-8 (1.2 fold), CXCL10, NF-κB, and TNF-α. CPEb promoted 2-NBDG absorption in differentiated C2C12 myotubes under both basal (16.08%) and insulin-stimulated (51.09%) conditions. CPEb increased IRS/AKT-dependent glucose absorption and also activates the AMPK pathway. Anti-α-glucosidase, anti-inflammatory and antioxidant activity of CPEb indicate their role in treating hyperglycemia by scavenging ROS and inflammatory responses.