International Journals

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Start date: 2020Has files: Yes

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    PHYTOCHEMICAL STUDIES OF ENDOLICHENIC FUNGI ISOLATED FROM HYPOTRACHYNA INFIRMA (KUROK.) HALE
    (International Journal of Pharmacy and Pharmaceutical Sciences, 2022-08) Gokilavani, R; Banu, H.R
    The aim of this study is to investigate of phytopharmaceutical importance of endolichenic fungi isolated from Hypotrachyna infirma (Kurok.) Hale. Methods: The lichen species were collected from Sholaiyar hills, Coimbatore and identified as Hypotrachyna infirma (Kurok.)Hale. From this lichen, 29 endolichenic fungi were isolated and 13 endolichenic fungi were identified. From the identified endolichenic fungi, 26 extracts were prepared by successive solvent extraction methods using Ethyl acetate and chloroform. Results: The phytochemical study revealed the presence of important constituents like Alkaloids, Tannins, Carbohydrates, Phenols, Protein, Terpenoids, Steroids, Glycosides Flavonoids and Saponins. From the 13 endolichenic fungi, only 5 endolichenic fungi (Nigrospora oryzae (Berkand Broome)Petch, Geotrichum candidum Link, Scytalidium lignicola pesante, Aspergillus oryzae(Ahlb.) cohn, Aspergillus niger Gr.) have more constitutents. These 5 endolichenic fungi have good results in Quantitative analysis also. Conclusion: Compared to ethyl acetate extracts Chloroform extracts showed very less concentration of the phytochemicals. From this study we conclutated Nigrospora oryzae (Berk and Broome) Petch gave the best results in both qualitative and quantitative compared to other endolichenic fungi.
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    SLOW-GROWTH CONSERVATION- CRYOPRESERVATION AND ANALYSIS OF GENETIC STABILITY OF IN VITRO REGENERATED COELOGYNE NERVOSA A.RICH A ENDEMIC ORCHID
    (International Journal of Research in Pharmaceutical Sciences, 2021-06) B S, Chithra Devi; A, Sangilimuthu; V, Daanya
    Coelogyne Nervosa A. Rich., an endangered medicinal epiphyte (lithophyte) is a strict endemic of the Western Ghats distributed across Karnataka, Kerala and Tamil Nadu. KC medium supplemented with 2,4-D is best suited for direct embryo induction from 80 days old protocorms. Hormone-free KC basal medium supported the conversion of somatic embryos into complete plantlets. For the medium-term storage experiment, somatic embryos were placed on storage media containing various growth inhibitors (such as different levels of chlormequat, maleic hydrazide and paclobutrazol). They were stored for ten months to assess the percentage of survival. The surviving shoots were transferred to the recovery medium for eight weeks. At 8°C, the highest survival rate was observed with ABA at 0.2 mg L−1. For cryopreservation: droplet-vitrification best results were obtained when the somatic embryos, after 20 days of subculture were immersed in an osmoprotectant solution. Osmo-protected samples were immersed in PVS2 (15% ethylene glycol and 15% DMSO) at 0°C for 30 min. Pre-prepared Somatic embryos were plunged into cryovials for one day. Regrowth levels were as high as 70%. DNA samples extracted from leaves of the mother plant, somatic embryos without storage, regenerates of slow growth preservation and cryopreservation. The samples were analysed by DNA based molecular marker – inter simple sequence repeats (ISSR) and found no genetic changes during the cryopreservation process.
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    EFFECTS OF ALCOHOLISM ON NUTRITION AND RECOVERY USING NUTRITION MANAGEMENT IN ROMA TOWNSHIP- LUSAKA
    (Eureka Journals, 2023) Sakthivel R; Subhashini S
    Alcohol is a product that has provided a variety of functions for people throughout all history. From the ancient times to the modern world, alcohol has played ansignificantrole in religion and worship. In the past, alcoholic beverages have been provided as sources of requirednutrients and have been widely utilizedas for their medicinal, antiseptic and analgesic properties. The aim and objective of this project was to look at nutritional problems created by consumption of alcoholic beverages, to look at the effect of alcohol on vitamins and to show how ones genes will affect their response to alcohol. This research was conducted in Serenity Harm Reduction Program Zambia (SHARPZ).
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    PHYTOCHEMICAL STUDIES OF ENDOLICHENIC FUNGI ISOLATED FROM HYPOTRACHYNA INFIRMA (KUROK.) HALE
    (Innovare Academic Sciences Pvt Ltd, 2022) Gokilavani, R; H, Rehana Banu
    The aim of this study is to investigate of phytopharmaceutical importance of endolichenic fungi isolated from Hypotrachyna infirma (Kurok.) Hale.The lichen species were collected from Sholaiyar hills, Coimbatore and identified as Hypotrachyna infirma (Kurok.)Hale. From this lichen, 29 endolichenic fungi were isolated and 13 endolichenic fungi were identified. From the identified endolichenic fungi, 26 extracts were prepared by successive solvent extraction methods using Ethyl acetate and chloroform.The phytochemical study revealed the presence of important constituents like Alkaloids, Tannins, Carbohydrates, Phenols, Protein, Terpenoids, Steroids, Glycosides Flavonoids and Saponins. From the 13 endolichenic fungi, only 5 endolichenic fungi (Nigrospora oryzae (Berkand Broome)Petch, Geotrichum candidum Link, Scytalidium lignicola pesante, Aspergillus oryzae(Ahlb.) cohn, Aspergillus niger Gr.) have more constitutents. These 5 endolichenic fungi have good results in Quantitative analysis also.