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Author: search.filters.author.Vasandha, S

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    ZINC SOLUBILIZING BACTERIAL ISOLATES FROM THE AGRICULTURAL FIELDS OF COIMBATORE, TAMIL NADU, INDIA
    (Current Science, 2016-01) Sunithakumari, K; Padma Devi, S N; Vasandha, S
    Zinc plays a pivotal role in physiological and biochemical functions of the plants. Both quantitative and qualitative yield of the plants are strongly dependent on this micronutrient. Supplementation of zinc in the form of synthetic fertilizer is proved to be inappropriate due to its unavailability to plants. This crisis can be prevented by the identification of rhizospheric micro-organisms which has the potential to transform various unavailable forms of the metal to available forms. In the present study about thirty five zinc solubilizing bacteria were isolated from eight different agricultural fields (banana, chilli, field bean, ground nut, maize, sugarcane, sorghum and tomato) in and around Coimbatore district of Tamil Nadu. Five isolates were selected as best strains based on their solubilization efficacy in the qualitative estimation. The selected five isolates were identified using 16S rRNA as Stenotrophomonas maltophilia (ZSB-1), Mycobacterium brisbanense (ZSB-10), Enterobacter aerogenes (ZSB-13), Pseudomonas aeruginosa (ZSB22) and Xanthomonas retroflexus (ZSB-23). These strains were subjected to further studies such as quantitative estimation, influence of the isolates on the pH of the medium and production of gluconic acid as well as IAA. Of the five bacterial isolates, Pseudomonas aeruginosa showed maximum solubilization of zinc in the broth and also maximum decrease in the pH from 7 to 3.3 and recorded highest IAA production. HPLC analysis of gluconic acid production by the selected isolates indicated their potential to solubilize zinc.
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    ASSESSMENT OF COMPETENCE OF THE PSEUDOMONAS AERUGINOSA TO SOLUBILIZE INSOLUBLE FORM OF ZINC UNDER VARIOUS CULTURAL PARAMETERS
    (Springer Link, 2016) Padma Devi, S N; Sunitha kumari, K; Vasandha, S
    Efficiency of Pseudomonas aeruginosa to solubilize insoluble form of zinc to soluble form was studied under various cultural parameters such as carbon (glucose, fructose, sucrose, maltose and lactose), nitrogen (ammonium sulphate, sodium nitrate, potassium nitrate and urea), pH (5.0, 7.0 and 9.0) and temperature (20, 30 and 40°C). The experimental study was carried out by both qualitative (plate assay) and quantitative (broth assay) methods. The Zn-solubilizing efficiency of the isolate was found more when glucose was added as C-source in both plate (150 %) and broth (16.62 mg/l) assays, and the efficiency was less in the medium amended with sucrose as carbon source in both qualitative (105.8 %) and quantitative (2.56 mg/l) estimation. Among the four nitrogen sources, P. aeruginosa showed best solubilizing efficiency (262.5 %) and solubilization of Zn (29.91 mg/l) in the presence of ammonium sulphate and recorded least solubilization efficiency in the presence of sodium nitrate as nitrogen source in both plate (181.82 %) and broth (21.92 mg/l) assays. It showed highest solubilization of zinc from ZnO at the incubation temperature of 30°C, and the activity was less at the temperature of 40°C. pH 7 was the most favourable pH for solubilization, and the efficacy was least when the pH reached its alkalinity. Thus the study confirmed the efficiency of P. aeruginosa to solubilize insoluble form of zinc and can be used as a source of bioinoculants to eradicate zinc deficiency in plants.