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Item CORRIGENDUM TO ‘VOLTAGE-GATED CALCIUM CHANNEL BLOCKERS DEREGULATE MACROAUTOPHAGYIN CARDIOMYOCYTES’ [INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY(Elsevier, 2016-01) Charumathi, Pushparaj; Arindam, Das; Rosa, Purroy; Mireia, Nàger; Judit, Herreros; Reinald, Pamplona; Carles, CantíItem STUDIES ON THE EFFECT OF SIDA ACUTA AND VETIVERIA ZIZANIOIDES AGAINST THE MALARIAL VECTOR, ANOPHELES STEPHENSI AND MALARIAL PARASITE, PLASMODIUM BERGHEI(Rishan Publications, 2014-03-26) Narasimhan, Aarthi; G, James Pitchai; Kadarkarai, Murugan; Pari, Madhiyazhagan; Thiyagarajan, Nataraj; Arjunan, Nareshkumar; Kandasamy, Kalimuthu; Jiang-Shiou, Hwang; Donald R, Barnard; Hui, Wei; R, Chandrasekar; A, AmsathThe methanolic extracts of Sida acuta and Vetiveria zizanioides leaves and root was investigated for antimalarial activity against Plasmodium berghei infections in mice. The median lethal dose was determined to ascertain the safety of the extract in mice. The antimalarial activities during early and established infections were evaluated. Phytochemical screening was also investigated to elucidate the possible mechanism of the antimalarial and antivectorial properties. The extracts of Sida acuta and Vetiveria zizanioides leaf and root demonstrated that a significant antiplasmodial activity in all the three groups (test for root and leaves and one control includes three groups) of the antimalarial evaluations. Plant extracts treatment showed higher mortality against mosquito larvae, lethal dose (Lc50 and Lc90) was also worked out for the larval instars of malarial vector, Anopheles stephensi. Phytochemical screening revealed that the presence of some vital insecticidal and antiplasmodial constituents such as terpenoids, flavonoids and alkaloids. The leaf and root extract of S. acuta and V. zizanioides showed markedly significant antimalarial activity and antivectorial activity effects even at low concentrations. O S. acuta and V. zizanioides are promising in mosquito control and also safe for the non-target organisms. This integrated application could be useful as alternative synthetic insecticides. These agents should preferentially to be applied in mosquito control strategies to reduce the mosquito populations and prevent the malaria.Item EVALUATION OF LARVICIDAL ACTION OF NATURAL EXTRACTS ON MOSQUITO LARVAE OF AEDES AEGYPTI (DIPTERA: CULICIDAE)(Innovative Journal Solutions, 2016) Susheela P; Radha R; PadmapriyangaMosquitoes are known to cause more diseases than any other group of arthropods and affect millions of people throughout the world. The current study was aimed to investigate larvicidal potential of three natural extracts such as aloe vera and onion, alcohol and cloves, marigold and garlic against the dengue vector, Aedes aegypti. Insecticidal susceptibility tests were carried out using WHO standard method and the mortality was observed after 24-h exposure. All the tested extracts showed moderate to good larvicidal activities. However, the maximum larval mortality was observed in the extract of the alcohol and cloves that showed high efficacy on the Aedes mosquito larva. The findings of the present studies suggested that the use of aloe vera, onion and garlic has a strong effect in acting as a mosquito larvicide and the natural plant extracts can be prepared at home and used at any cost of time which is feasible for the massesItem STUDIES ON THE LIFE HISTORY AND OVIPOSITIONAL PREFERENCE OF CALLOSOBRUCHUS MACULATUS REARED ON DIFFERENT PULSES(International Science Community Association, 2014) Radha R; Susheela PThe pulse beetle, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), is a worldwide insect pest that infests pulses in the fields and seeds in storage. An investigation has been conducted on the life history and ovipositional preference of Callosobruchus maculatus reared on five different pulses. The oviposition period had not varied significantly between the pulses. The adults exhibited a marked preference for smooth, well-filled seeds for oviposition.The order of preference for Callosobruchus maculatus for oviposition were: Vigna unguiculata (cowpea) > Vigna radiata (green gram) > Vigna mungo (black gram) > Dolichos biflorus (horse gram) > Pisum sativum(green peas). The pulses selected for the investigation were initially infested by the insect, but the degree of infestation varied among the pulses. This is due to the varied responses of the insect to different host seeds for oviposition and their appropriateness for the successful completion of the life cycle.Item LABORATORY EVALUATION OF THE BIOCHEMICAL PARAMETERS IN THE HAEMOLYMPH OF THE LEPIDOPTERAN LARVAE AFTER STINGING BY THE POTTER WASP, EUMENES CONICA (INSECTA: HYMENOPTERA)(Longdom Publishing, 2014) P, SusheelaPotter wasps belong to the subfamily Eumeninae of the family Vespidae. Potter wasp is a common name given for a group of caterpillar hunting wasp which builds the pot-shaped mud nests. Initially the wasp constructs the nest and then starts hunting for its prey, the caterpillars. The prey is stung and paralyzed by the wasp then brought to the nest. It is perhaps a very highly specific behavior of these wasps. The female wasp lays her egg on the prey. The egg is firmly attached to the prey and the larva hatching out sucks the haemocoel which oozes out from the prey. In the present study, the biochemical changes in the haemolypmh of Helicoverpa armigera (Lepidoptera: Noctuidae), before and after stinging by the potter wasp were observed. There is considerable change in the nutritional physiology of parasitized prey. The successful development of the parasitoid depends on the concentration of the host haemolymph. Hosts do not survive and thus parasitoids play an important role in regulating the population of the hosts.Item IN-VITRO ANTIBACTERIAL ACTIVITY OF ZINC AND COPPER NANOPARTICLES AGAINST GRAM-NEGATIVE BACTERIA AEROMONAS HYDROPHILA(Malaya Journal of Biosciences, 2014-11-18) Thirunavukkarasu Muralisankar; Periyakali Saravana Bhavan; Selvaraj Gandhimathy; Subramanian Radhakrishnan; Chandirasekar Seenivasan; Palani AarumugamThe present study was focused to investigate the in-vitro antibacterial activity of bulk and nano forms of zinc (Zn) and copper (Cu) against the Gram-negative pathogenic bacteria Aeromonas hydrophila. The in-vitro antibacterial activities of these metals were determined by well diffusion method. The pure cultures of A. hydrophila were sub cultured on nutrient agar medium and swabbed uniformly onto nutrient agar plates and 6 mm wells were punched into the each nutrient agar plates. Zn, Zn nanoparticles (ZnNPs), Cu and Cu nanoparticles (CuNPs) were suspended in double distilled water using ultra sonicator. Further, the each suspended metals were poured onto each respective wells at the concentrations of 100 µg, 200 µg and 400 µg. The wells without any metal were served as control. The plates were incubated for 24 h at 35-37°C and the antibacterial activity was determined by zone of inhibition in mm. The zone inhibition of A. hydrophyla was observed in all treated wells with both bulk and nanosized metals. Among these metal forms, CuNPs treated well showed more inhibition in all three concentrations followed by Cu, ZnNPs and Zn, whereas, the control wells of the culture plates did not showed any zone inhibition. This study indicates that nano forms of Zn and Cu have more growth inhibition against A. hydrophila. Hence, it suggests that these ZnNPs and CuNPs can be used as antibacterial agent against A. hydrophila.Item REVELATORY NOTE ON BULBOPHYLLUM FIMBRIATUM – AN ENDEMIC ORCHID OF WESTERN GHATS, INDIA(Journal of Economic and Taxonomic Botany, 2019-07-24) K, Kiruthika; M, Sulaiman; P B, Harathi; R, GopalanCirrhopetalum is a curious and elegant orchid easily identified by its lateral connate sepals. This peculiar characteristic genus is made as a section of Bulbophyllum. A collection of an endemic orchid, Bulbophyllum fimbriatum recorded the distribution in Tamil Nadu, India.Item EFFICACY OF PLEUROTUS SAJOR CAJU AS AN EXCELLENT POTTING MEDIUM FOR GARDEN PLANTS(Int J Pharm Bio Sci, 2016-01) Harathi P.B; Radha R; Gayathri MCoir is a natural fibre found between the hard, internal shell and the outer coat of a coconut is used as a by-product of the coir manufacturing industry. Normally, they are dumped as agricultural wastes in the fields or disposed by burning which causes air pollution. Composting of coir pith is an alternate method to reduce pollution and it also serves as a source for the maintenance of organic matter of the soil. Coir pith is an ideal soil re-conditioner and soil substrate with excellent water holding capacity. Coir pith contains high quality of nutrients which keeps the soil healthy in a natural way. It acts as a top dressing that helps to maintain moisture content of the soil and re-conditions the soil. The proportion yielded effective growth of the plant and indicating its use as an excellent potting media for garden plants. Hence the objective of the present study focused on the efficacy of basidiomycetes fungus, Pleurotus sajor-caju for the cultivation of medicinal plant, Ocimum santum using the biodegraded coir pith.Item SPACE TIME MODEL FOR CANCER INCIDENCES IN TAMIL NADU: MAPPING HEALTH STATISTICS FOR POLICY PROGRAMMING AND DECISION MAKING(International Journal of Advanced Research in Computer Science and Software Engineering, 2015-04) P B, Harathi; Janani Selvaraj; M, Prashanthi DeviThe burden of cancer is growing globally and is one of the top leading causes of death. Information on cancer patterns is essential for effective planning of cancer control interventions. In specific the geographical study of cancer will help in identifying the high risk communities for further etiological studies. The objective of the present study is to analyze the time based geographical expansion of cancer incidences in the study region. The spatialtemporal model using Knox and Mantel statistic was applied to identify if additional cases are added in subsequent time period from high incidence areas or from moderate areas or from low incidence areas. This study will provide an indication to any association between time trend and cancer incidences. Through the spatial temporal model, the high risk areas have been identified and the temporal variations in the risky zones were assessedItem STUDIES ON THE BACTERIOLYTIC ACTIVITY OF SERUM OF MUD CRAB, SCYLLA SERRATA AGAINST THREE DIFFERENT STRAINS OF BACTERIA(International Journal Of Current Research, 2014-12-30) P B, Harathi; R, RadhaInvertebrates are dependent on cellular and humoral immune defences against microbial infection. Scylla serrata is an important commercial species of crab, but the fundamental knowledge on its immune defense related to the immune-associated reactions is still lacking. This study was undertaken to determine the antibacterial activities of serum from the mud crab, Scylla serrata against three different bacterial strains. Bacterial cultures were treated with different volumes serum from Scylla serrata and the growth was monitored by optical density at 450 nm. In addition, the serum was treated with protease to determine the mechanism of antibacterial activities. Treatment of bacterial cultures with serum from mud crab, Scylla serrata resulted in a volume-dependent decrease in bacterial growth. Cultures of M. lysodeikticus, exhibited strong growth inhibition by serum of Scylla serrata, while cultures of Vibrio furnissi and Vibrio damsel were nearly completely obliterated for 24 h by only 10% (v/v) serum. The antibacterial activity of Scylla serrata serum occurred very rapidly, as 18% of M. lysodeikticus growth was inhibited by a five min exposure to serum. Furthermore the bacteriolytic activity detected in the presence of phosphate buffer was significantly higher than that observed with, thereby indicating the suitability of phosphate buffer for assay of bacteriolytic activity.