National Conference
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Item ESTIMATION OF PROTEIN AND CARBOHYDRATES IN DIFFERENT PULSES(PSGR Krishnammal College for Women, 2016-01-05) Ashiba A; Jeeva Lavanya S; Menachi A; Shifana Asmi M; Soundarya S; Vadivukkarasi K; Sumathi RPulses are essential for growth and metabolism of all living organisms. Leguminous crops are rich in protein and carbohydrates. The samples are soaked entire seed, seed coat, pulp, soaked water and also in boiled entire seed, seed coat, pulp and boiled water of black channa, white channa, fresh bean, dry bean, fresh pea and dry pea. The protein content was estimated by Bradford method and carbohydrate content was estimated by Anthrone method. The protein and carbohydrates are rich in boiled seeds than soaked seeds. The result shows the consumption of the boiled pulses is recommended and it is good for health.Item EXTRACTION OF AMPELOCISSUS LATIFOLIA AS A SOURCE OF ANTIBIOTIC(Muthayammal College of Arts & Science, Rasipuram, 2015-01-09) Sumathi R; Renuka Devi RThe Antifungal activity of different solvent extract of leaf and root powder of Amplelocissus latifolia was evaluated against Aspergillus niger and Penicillium candidum. The methanol, ethanol, hexane and acetone extract showed no zone of inhibition against both the organisms. The presence of protein was identified in phosphate buffer extract using Nin hydrin test and ELISA. The leaf and root extract contains 7.6ug/ul and 7.7ug/ul of protein respectively. The phytochemical studies showed the presence of Alkaloids, glycosides, carbohydrates, tannins and phenols in the leaf extract. The root extract contains only flavonoids . The FTIR analysis of root and leaf powder of Amplelocissus latifolia showed the presence of amines, amides alcohols carboxylic acids, nitric acids and phenolsItem BIOPLASTICS– UTILISATION OF VEGETABLE AND FRUIT WASTE FOR THE USE OF GREEN APPLICATIONS(Gujarat Institute of Desert Ecology, Bhuj, 2020-03-07) Rubalakshmi S; Uma EThe major sources of land pollution include plastics, metal and glass containers, food wrapping, worn-out machinery, old furniture, garbage, etc. Plastics have become a large environmental problem. However, it is slow to degrade. The plastics in excess produced will be deposited as landfill and are degraded very slowly, which can cause the original products to remain in the landfills for hundreds or even thousands of year. The prominence of plastic pollution is correlated with plastics being economical and durable, which lends to high levels of plastics used by humans and plastic pollution, can unfavorably affect lands, waterways and oceans. In order to reduce the usage of Plastics, currently Bioplastics are produced from the agricultural and vegetable wastes. Bioplastics are plastic materials produced from renewable biomass sources, such as vegetable fats and oils, corn starch, straw, woodchips, sawdust, recycled food waste, etc. Bioplastic can be made from agricultural by-products and other containers using microorganisms. At present we could see the use of conventional plastic in every corner of the world, but their use raises serious environmental issues and public irritation because of their non-degradable nature. Hence, nowadays it is indispensable to have a potential bioplastic material in alternate over the conventional plastics. The bioplastic obtained will be environmentally friendly, trendy, user friendly and degradation tractable properties. There were plenty of reports on bioplastic synthesis using vegetable and fruit waste, however, there are less reports on tractability biodegradation, thus produced biodegradation tractable plastic could play vital role in the market for the sustainable use and commercial value added product development. The bioplastic produced through this method could be substantial and the biodegradable tractability is one of the main challenges in developing bio plastic material. The current report has made an effort towards the synthesis and characterization these types of natural polymeric material. Certainly, the research is a long way to go for both economic and environmental friendly products using bioplastic materials or bio polymer.Item PHYSICO - CHEMICAL PARAMETERS OF TEXTILE DYE EFFLUENTS AND SOILS COLLECTED FROM TIRUPUR DISTRICT, TAMIL NADU(Department of Bioscience, Sri Krishna Arts and Science College Coimbatore, 2017-02-10) A, Jeevitha; S, Mohanapriya; L, Pavithra; V, Deepthi; M S, Sri Saranya; S, Srimathi; E, UmaTextile industries are large industrial consumers of water as well as producers of wastewater effluents. The continuous application of effluent on soil and water may change its quality as well as its fertility. In this study the physico chemical parameters such as colour, pH, Electrical conductivity (EC), Total solids, Total suspended solids (TSS), Total dissolved solids (TDS), Total organic carbon (OC), Biological oxygen demand (BOD), Chemical oxygen demand (COD), Total nitrogen, Phosphate, Exchangeable Potassium, Exchangeable Sodium of the untreated textile effluents were analyzed. In this regard, the result indicates that the physico-chemical properties of industrial effluent are higher than the permissible limits therefore it affects the nature of soil and water. Hence there is a need of advanced methods of treatment of effluent water so that the waste water generated as the product of treatment can be reused for various biological meansItem MICROBIAL DEGRADATION OF LOW-DENSITY POLYETHYLENE (LDPE) BY FUNGUS ISOLATED FROM LANDFILL SOIL(Avinashilingam institute for home science and higher education, Coimbatore, 2019-12-18) Jeeva Dharshni S; Kanchana MPlastics are natural or synthetic represent the class of polymeric substances. A stable increase in the use of plastic products has accelerated the pollution. Several attempts have been made to control the problem at large by resorting to both chemical and biological methods. Eradication of pollution by chemical methods leads to many side effects such as, release of toxic chemicals and gases into the atmosphere. Therefore an alternative method must be identified and it should be eco-friendly manner. Hence a current study is focused on identify the biological method for the degradation of plastics without any side effects. Low density polyethylene polymer can be degraded by microorganisms or enzymes by means of cutting down the molecular chains. A low density polyethylene (LDPE) is one of the hazardous polymers used for the present study and makes it degrade fully in a rapid manner. There are two fungal strains Aspergillus sp. and Xylaria sp. were identified for their high degradable activity. After 30 days of incubation the efficacy of fungal isolates for their polymer degradation was determined through various parameters such as pH variation, Optical Density of the culture and estimation of CO2 during degradation process.Item FLORISTIC DIVERSITY AND ETHNOMEDICINAL ANALYSIS OF ANGIOSPERMIC PLANTS IN KUMARANKUNDRU HILL, ANNUR, COIMBATORE(St.Xavier’s College, 2019-11-28) Preethi Jenifer S; Lekha K; Kanchana MTaxonomy is the study to gather acquaintance of various kinds of plants in earth and its systematic grouping, the main aim of taxonomist to deal with the identification, nomenclature and classification of plants in scientific manner. The present study is focused on taxonomic and comprehensive information of the floristic diversity and Ethnomedicinal properties of Angiospermic plants found in Kumaran kundru hill surrounded by Western Ghats. A total of 56 families, 114 genera and 126 plant species were recorded. All collected plants were preserved by using herbarium techniques. The vegetative survey conducted to the show level of species diversity, it reveals that Ricinus communis, and Boerhaviaerecta have high species diversity while Sarcostemmaviminalis , Agave americana have low species diversity. Documentation of established knowledge on the Ethnomedicinal properties of these plants is essential for the conversation and new drug development processItem SCREENING AND IDENTIFICATION OF IAA PRODUCING BACTERIA(St.Xavier’s College, 2019-11-28) Sujithra V; Kanchana MBiofertilizers are the substances which make use of microorganisms to fertile the soil. The plant growth promoting bacteria was act as biofertilizer to enhance the plant growth. These types of microorganism are used to increase the level of nutrients in the plants. They let the plants into a healthy environment and do not cause the pollution. Seven bacteria strains isolated from rhizospheric soil of Spathaglottisplicata.The screening and isolation of plant growth promoting bacteria was subject to confirm through the Salkowshi reagent. The pink color indicated the presence of IAA in plant growth promoting bacteria. Out of 7 bacterial isolates 3 bacterial cultures were produced high amount of auxin content. The plant growth promoting bacteria were identified by morphological analysis [gram stain, cell shape, size and motility] and biochemical analysis [IMVIC Tests].Item BIODEGRADATION OF LOW DENSITY POLYETHYLENE (LDPE) FILMS USING BACTERIA ISOLATED FROM PLASTIC DUMPED SOIL(St. Xavier’s College (Autonomous), Palayamkottai, 2019-11-28) Jeeva Dharshni S; Kanchana MPlastic are one of the most widely used materials and in most cases, they are designed to have long life time. Though plastics have become cosmopolitan, now days, they are very hazardous and should be disposed off, properly. Land filling, incineration and recycling are the most common methods employed for the disposal of plastics and all methods have their own environmental and health hazards. So, biodegradation will be the right choice for the proper disposal of plastic wastes. Soil samples from the compost yard have the rich consortia of biodegrading microbes. These samples were inoculated into nutrient agar medium with plastic as the sole carbon source for the isolation of the plastic degrading strains. Characterization of bacterial strains was done based on morphological, biochemical and molecular characterization. The process of biodegradation was observed at regular interval for 90 days in a synthetic medium containing Low Density Polyethylene (LDPE) films. The bacterial isolates,Bacillus spandPseudomonas spwere able to reduce the weight of the polymer up to 0.3% and 0.6% respectively. Degradation was monitored by observing weight loss and changes in physical structure by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) Spectroscopy.Item MOLECULAR CHARACTERIZATION OF TYPE 1 ACYL COA: DIACYL GLYCEROL ACYLTRANSFERASE (DGAT1) GENE IN BRASSICA NAPUS(ST.XAVIER’S College, Palayamkottai, 2019-11-28) Geetha M; Kanchana MOil in the form of triacylglycerols (TAGs) is quantitatively the most important storage form of energy for eukaryotic cells. Diacylglycerol acyltransferase (DGAT) is considered the rate-limiting enzyme for TAG accumulation. DGAT1 enzymes (acyl-CoA:diacylglycerol acyltransferase1) catalyse the formation of triacylglycerols (TAGs), the most abundant lipids in vegetable oils. Thorough understanding of the enzymology of oil accumulation is critical to the goal of modifying oilseeds for improved biodiesel production. Brassica napus, one of the world’s most important oil seed crops and is a bright-yellow flowering member of the family Brassicaceae (mustard or cabbage family). B.napus is promising candidate for biodiesel production. The DNA extracted from selected plant sample and the DGAT gene has amplified through PCR techniques. The PCR amplification product having approximately 1500bp was sequenced edited and searched using BLAST against the known sequences within NCBI databases.Item STANDARDIZATION OF AGROBACTERIUM MEDIATED GENE TRANSFER PROTOCOL IN PARADISE TREE(Rathinam College of arts and science, CBE, 2019-02-27) Geetha M; Kanchana MSimarouba glauca belongs to the family of Simaroubaceae. It is a medium sized evergreen tree and medicinally important plant. Simarouba glauca oil seed is used as an alternative energy to replace fossil fuel. The chemical constituents of Simaroubaare used as antiprotozoal, anti-amebic, antimalarial and it is even toxic to cancer and leukemia cells. In this study, an efficient and improved micro propagation protocol will be developed by the optimization of culture condition using MS medium. Micro-propagation is an effective protocol which gives rapid multiplication in a shoot cycle and it results in increased number of seedlings. The advantage of In vitro propagation is production of pathogen free elite cultivars. In this context the pathogen free plants of their genotype and subsequent phenotypes will be improved by introducing the desired gene through molecular cloning. Since, the time duration taken for the growth of tree is more than 10 years, it could be reduced through genetic engineering by introducing genetically modified elite cultivar. Hence, the present research programme will be a promising initiative for the future development of economically important bio resources. Agrobacterium mediated transformation system is an efficient, harmless, ecofriendly with high transformation efficiency than the other gene delivery system.