Browsing by Author "Parthi N"

Now showing 1 - 9 of 9

DESIGN OF AN AIR-EXPOSURE AND SUBMERGENCE STRESS NON-SPECIFIC IMMUNE RESPONSE MODEL IN THE EXOTIC FISH, OSPHRONEMUSOLFAX
(Department of Biotechnology, PSG College of Technology, Coimbatore Tamil Nadu, India, 2004-02-15) Jacintha S; Natarajan G M; Esther Joice P; Parthi N; Sasikala G; Tamilselvi G
The effects of air-exposure (6h) and submergence (6h) as monitored by determining RBC, Hb, Ht and blood glucose were analyzed in the exotic fish, Osphronemusolfax. Results obtained using multifactorial stress model (air-exposure, submergence, hypoxia) showed that elevation of RBC, Hb, Ht and blood glucose was linearly correlated with progressive stress uniformly. Submergence caused rapid stress with blood glucose reaching 50% elevation within 60 min. Hypoxic water with access to air had very little effect on blood glucose. Similarly, lowering the temperature significantly affected the blood parameters. The airexposed model was also used for testing lysozyme activity and phagocytosis assay during stress. Contrary top blood glucose, plasma lysozyme was significantly lower in air stressed than in unstressed fish. Submergence increased the numbers of granulocyte, lymphocyte.
THE EFFECTS OF HYPOXIC STRESS ON THE BIMODAL RESPIRATION OF MACROPODUSCUPANUS
(Biocon Publications India Pvt., Ltd, Erode, Tamil Nadu, India, 2001-06) Esther Joice P; Mallikaraj D; Parthi N; Natarajan G M; Sasikala G; Tamilselvi G; Kasthuri M
Short term (48 h) hypoxic stress increased the aerial respiration and depressed the aquatic consumption. Long term (15 and 30 days) stress increased the aerial respiration and reduced the aquatic respiration. The total respiration declined at all times of exposure. It appears that respiratory depression is an effective strategy to combat hypoxic stress in Macropoduscupanus
ENVIRONMENT INDUCED CHANGES IN THE BIMODAL GAS EXCHANGE AND HAEMATOLOGY OF FACULTATIVE AIR-BREATHING FISH, MYSTUSPUNCTATUS
(Department of Biotechnology, PSG College of Technology, Coimbatore Tamil Nadu, India, 2004-02-15) Devika R; Natarajan G M; Parthi N; Esther Joice P; Jacintha S; Parimala K; Sasikala G
Many facultative air-breathing fishes augment their supply by utilizing a modified swim - bladder to breathe air. However, there is no direct evidence for the involvement of air bladder in the respiratory functions of any Indian air-breathing fish. Therefore, the present investigation was undertaken to study the environment-induced changes in the bimodal gas exchange and blood characteristics of Mystuspunctatus. Fish is a facultative air-breather. The air bladder extracts 27% of Оxygenfrom air under bimodal conditions. One-hour air exposure increased the air bladder Оxygenconsumption to 39%. 96 h starvation increased the gill respiration (70.5%) 35°C exposure increased the airbladder uptake to 34.18%. 96 h hypoxic exposure increased the gill respiration to 70.19%. Except blood glucose, RBC, Hb, Ht, О capacity, MCV, MCHC and MCH are more in male fishes than the females. One hr air- exposure decreased the, RBC, Hb, Ht, Оxygen capacity and blood glucose. Air exposure stress increased the MCV, MCHC and MCH. Present study demonstrates that environmental stress has pronounced effect on the bimodal exchange and hematology of Mystuspunctatus.
FIRST REPORT ON THE ENVIRONMENTAL RECONSTRUCTION OF DEVONIAN PERIOD COIMBATORE CITY AS REVEALED BY THE FIRST PLANT FOSSIL FINDING
(Department of Biochemistry and Biotechnology, Avinashilingam Deemed University, Coimbatore, Tamil Nadu, India, 2002-12-12) Natarajan G.M; Parthi N; Santhi P; Krishna Kumar N; Palanisamy P; Bhuvaneshwari N; Sasikala G; Rajamamannan M.A; Parimala K
First report for the first time we report the occurrence of a rare fossil plant in Coimbatore. The present plant fossil was obtained from four different places in the heart on city town during foundation digging for apartment construction activities. Perusal of the literature showed that these fossils were reported only from Australia and other places. With the help of the present fossil finding an attempt is made to reconstruct the Devonian period environment of Coimbatore city.
MOLECULAR DEFENSE STRATEGIES IN CLARIAS DURING AIR-EXPOSURE AND SUBMERGENCE STRESS
(Department of Biotechnology, PSG College of Technology, Coimbatore Tamil Nadu, India, 2004-02-15) Esther Joice P; Jacintha S; Parthi N; Sasikala G; Tamilselvi G; Natarajan G M
The ability of fish colonize a large variation of biotopes is integrally related with the striking molecular and functional differentiation in their enzyme systems. Clariasgariepinus is an ideal model for investigating molecular adaptations in enzyme systems to extreme environmental conditions. The fish is an air breathing fish with branched gill filaments, thick gill rods, and fused secondary lamellae, all indicating that the fish is poorly adapted for water breathing life. Generally, for fishes, air exposure involves many biochemical changes like respiratory acidosis, alkalosis, ammonia excretion and blood pressure changes. Increasing vascular resistance and collapse of gills due tom gravity is also a serious problem. Similarly, forced submersion induces bradycardia, slowing of heart rate and decrease of blood oxygen level. A number of enzyme systems and molecular mechanisms mediate these defense strategies. In Clariasgariepinus, 9 h air-exposure and 24 h submersion unfolds many enzymatic hanges. Protein synthesis in heart, liver, kidney, brain, muscle, stomach and eyeballs is severely altered the acid phosphatase content of stomach and eyeballs increased significantly (P<0.05). All other organs registered depression. The alkaline phosphatase activity of gills, art, liver, kidney, brain, muscle and stomach exhibited suppression. Significant cumulations (P<0.05) of Na K" ATPase in the gills, kidney and eyeballs were recorded. Ca dependent ATPase increased in the heart, muscle, stomach and eyeballs. The ATPase is elevated only in the eyeballs.Since stress induced anoxia in the cells, the aerobic energy input is severely impaired gesting drastic reductions in the various forms of the cell work. In the present work, lies on brain in particular, but also on liver cells, have documented reductions in severalkey processes that drive ATP utilization, transmembrane ion leakage, electrical activity, and protein synthesis. In the language of the field, these have been referred to respectively as channel arrest, spike arrest and transnational arrest (Hochachkaet al., 1996). The molecular implications of these enzyme modulations are discussed.
NITROGEN EXCRETION IN THE FEATHERBACK, NOTOPTERUSNOTOPTERUS (PALLAS)
(Department of Biochemistry and Biotechnology, Avinashilingam Deemed University, Coimbatore, Tamil Nadu, India, 2002-12-12) Vijayalakshmi P; Parthi N; Natarajan G M; Mallikaraj D; Esther Joice P; Jacintha S; Bhuvaneshwari N; Sasikala G; Palanisamy P
Notopterus is predominatlyammoniotelic excreting 78.12% of waste nitrogen as ammonia and 21.62% as urea nitrogen. Mucous was the main route for the excretion of ammonia and urea. Emersion enhanced the excretion of ammonia and urea. Prolonged emersion stimulated the excretion rates of ammonia and urea. Aerial exposure significantly increased the blood and liver ammonia and urea content.
ON THE AESTIVATION OF MACROPODUSCUPANUS
(Biocon Publications India Pvt., Ltd, Erode, Tamil Nadu, India, 2002-06) Mallikaraj D; Esther Joice P; Parthi N; Natarajan G M; Sasikala G; Tamilselvi G; Kasthuri M
Extensive information are made available on the aestivation of air .breathing fishes (Smith, 1961; Natarajan, 1985 ; Mckenizle and Randall, 1990) but practically nothing is known on the aestivation of Macropoduscupanus. In this communication, the results of some investigations on the aestivation of Macropoduscupanusare described as it is known to have acquired the faculty of surviving period of severe drought.
ON THE FUNCTION OF LABYRINTHINE ORGAN OF MACROPODUSCUPANUS
(Department of Biochemistry and Biotechnology, Avinashilingam Deemed University, Coimbatore, Tamil Nadu, India, 2002-12-12) Natarajan G M; Parthi N; Esther Joice P; Mallikaraj D; Jacintha S; Bhuvaneshwari N; Sasikala G; Palanisamy P
The labyrinthine organs of Macropoduscupanus are shown to be effective in gas exchange during bimodal breathing. Under completely aerial conditions, significant participation of the labyrinthine organ in gas exchange is doubtful. Amputation of the labyrinthine organ increases the air breathing frequency. The period of survival out of water in amputated fishes is limited. The labyrinthine organ promotes trapping and retention of air in the suprabranchial chamber. It might also serve to minimise the evaporation of water from the labyrinthine organ.
STRESS INDUCED MOLECULAR AND BIOCHEMICAL MECHANISMS IN THE AIR-BREATHING EEL AND THEIR POTENTIAL USE FOR LIVE FISH STORAGE AND TRANSPORTATION
(Department of Biotechnology, PSG College of Technology, Coimbatore Tamil Nadu, India, 2004-02-15) Parthi N; Sasikala G; Natarajan G M
Eels are considered as a luxury food in several Asian and European countries. Modern eel culture exposes these fishes to various stressful conditions. The type of stress includesexternal factors such as air-exposures, submergence or handling disturbances. However, no information is available on the stress-induced changes on any parameters of this species. Eels of species Anguilla bengalensis were selected and subjected to the followingStress conditions: C- control (not exposed to any stress treatment) T1-5 h air-exposure stress T2-10 h air-exposure stress T3 – 6h submergence stress T4 -12h submergence stress. The effect of stress generally increases all the biochemical parameters during airexposure and submergence significantly (P<0.05) in the gills, heart, liver, kidney, brain, muscle, stomach and eyeballs. Pyruvate levels were also found to be increased in both he treatments. Decrease in glycogen and cholesterol levels were noticed in both the stresses. An irregular protein depletion and accumulation was recorded in the gills.Acid and alkaline phosphatases showed irregular decrease. The activity of Na,K ATPase found to be erratically accumulated in the eyeballs, gills and other tissues. Cat ATPase activity increased significantly (P<0.05) in the eyeballs. Other tissues exhibited either an increase or decrease in enzyme levels in all treatments. The ctivity of Mg** ATPase decreased in all the tissues except eyeballs and gills. While stress increased the RBC, Hb and Ht level, the clotting time decreased. Thrombocytes, MCV, MCH and MCHC showed either an increase or decrease. The decrease in the number of lymphocytes during stress and the gradual restoration to original level is definitely a hormone mediated biochemical change in haemopoetic stem cells. The present findings are valuable for live fish storage and transportation.