Department of Botany
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Item OPTIMIZATION OF PROCESS PARAMETERS FOR LIPASE PRODUCTION BY ASPERGILLUS TERREUS USING COTTON SEED OIL AS SUBSTRATE(SNMV College of Arts and Science, Coimbatore, 2009-01-28) Sumathi R; Meerabai R SFungi isolated from soil where screened for exogenous lipolytic activity. The highest lipase activity was found in an isolate of Aspergillus terreus. Optimal cultural conditions influencing the growth and production of extra cellular lipase from this fungus was investigated. The lipase yield was maximum on day 5 of incubation, when the medium was supplemented with maltose and cotton seed oil as carbon source and potassium nitrate as nitrogen source, at pH 4 and at a temperature of 400 C. The possibility of using the lipase thus produced for transesterification of cotton seed oil to biodiesel was investigated.Item BIODIESEL PRODUCTION FROM COTTON SEED OIL USING EXTRACELLULAR LIPASE ENZYME AS CATALYST(Advances in Plant Sciences, 2013) Sumathi R; Meerabai R SBiodiesel (Fatty acid methyl esters), which is derived from triglycerides by transesterification with methanol, has attracted considerable attention during the past decade as a renewable, biodegradable and nontoxic fuel. Several processes for biodiesel fuel production have been developed among which the enzymatic process offers several advantages than the chemical routes. Enzymatic transestrification using lipase has become more attractive for biodiesel fuel production, since the glycerol produced as a by product can be easily recovered and the purification of fatty acid methyl ester is simple to accomplish. The main hurdle to the commercialization of this system is the cost of lipase production. As a means of reducing the cost, the extra cellular enzyme is significantly advantageous. In the present investigation, optimization of process parameters for high lipase production by the microbes viz, Mucor racemosus, Aspergillus terreus and Penicillium candidum using SOB and MYGP medium were carried out. Culture filtrates were used as extra cellular crude enzyme source as a catalyst for conversion of cotton seed oil to biodiesel and the parameters such as quantity of enzyme and methanol needed for significant yield of biodiesel were standardizedItem LIPASE PRODUCTION BY ASPERGILLUS TERREUS USING COTTON SEED OIL AS CARBON SOURCE(Plant Archives, 2012) Sumathi R; Meerabai R SFungi isolated from soil were screened for exogenous lipolytic activity. The highest lipase activity was found in an isolate of Aspergillus terreus. Optimal cultural conditions influencing the growth and production of extra cellular lipase from this fungus was investigated. The lipase yield was maximum on day 5 of incubation when the medium was supplemented with maltose and cotton seed oil as sole carbon source and potassium nitrate as nitrogen source at pH 7 and at temperature of 40°C