Department of Botany

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Subject: search.filters.subject.Chlorophyll reduction

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    EFFECT OF SALT STRESS ON TOTAL CHLOROPHYLL CONTENT IN LEUCAS ASPERA
    (MacMillan New Delhi, 2017) Agnes Nirmala K; Kanchana M
    Leucas aspera is well known for its traditional uses as antipyretic and insecticide. It is commonly known as “Thumbai” and distributed throughout India from the Himalayas down to Srilanka. This plant possesses immense medicinal properties such as antifungal, antioxidant, anti-inflammatory, antimicrobial, antinociceptive and cytotoxic activity. Soil salinity is a very important issue affecting agriculture and over 800 million hectares of the world land are affected by salt stress. As Leucas aspera plant distributed throughout India, study on salinity tolerance of this plant may give more insights on abiotic stress research. The effect on photosynthesis can be gauged from the effect on the photosynthetic pigments and salinity stress significantly affected the chlorophyll content. In this study, the effect of salt stress on total chlorophyll content was analysed. The plant was treated with 150 mM NaCl and total chlorophyll content was estimated at 0 hrs, 12 hrs, 24 hrs, 48 hrs, 72 hrs and 120 hrs. We also estimated total chlorophyll content after three days with different salt treatments ranging 50 mM, 100 mM, 150 mM, 200 mM, 250 mM and 300 mM. The chlorophyll content significantly reduced after 72 hrs of 150 mM and higher salt concentrations. The chlorophyll content was not significantly affected up to 48 hrs in all salt concentrations analysed.
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    ISOLATION AND DIFFERENTIAL EXPRESSION OF SODIUM HYDROGEN ANTIPORTER (NHX1) GENES IN LEUCAS ASPERA
    (PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 2019-08-22) Kulandaisamy Agnes Nirmala; Marimuthu Kanchana
    Leucas aspera is a medicinal plant well known for its traditional uses such as antipyretic and insecticide. It is commonly known as Thumbai or Thumba and distributed throughout India from the Himalayas down to Srilanka. Soil salinity is an adverse environmental problem affecting agriculture and over 800 million hectares of the world land are affected by salt stress. To study the effect of salt stress on L. aspera, one month old seedlings were subjected to salt stress. The study showed a significant reduction of chlorophyll content after 72 hrs of treatment with 150 mM and higher salt concentrations. Vacuolar Na+/H+ exchanger (NHX1) is one of the key gene playing a vital role in salt tolerance. Bioinformatic tools were used to identify conserved region of NHX1 and to design degenerate primers. The NHX1 gene homologue was isolated and sequenced from L. aspera and submitted to NCBI (National Center for Biotechnology Information) database. The real time PCR based expression study showed significant upregulated expression of NHX1 after the plant subjected to salt stress upto 12 hrs but a reduction in expression was observed after 24 hrs. The results shows that the unsuccessful sequestration of sodium into vacuoles might be one of the reason for the sensitivity to salt stress by L. aspera.