Department of Botany

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    TEXTILE DYE DEGRADATION BY NEWLY ISOLATED BACTERIA AND DNA ISOLATION ASSOCIATED WITH DYE DEGRADATION
    (Kongunadu Arts and Science College, CBE., 2017-10-09) Geetha M; Kanchana M
    A bacterial strain with ability to decolorize or degrade textile dye was isolated from textile effluent contaminated soil of Tripur (Tamil Nadu). The decolorization or degradation studies were performed in Nutrient Agar medium (NA) amended with different textile dye, such as Spectron yellow F3RN, Spectron Navy CLBC and Spectron Rose F3BN. The bacterial strain was identified as Ochrobactrum atrophy on the basis of 16S rDNA sequence. The bacterial strain exhibited very low decolorization ability in static conditions. The optimal condition for the decolorization of three different dye by Ochrobactrum atrophystrain were at pH 7.0 and 35°C in 60 h of incubation. To identify the similarity between the microorganisms, the sequence was submitted in NCBI (national centre for biotechnological information). As a result the Ochrobactrum atrophy sequences of BLAST shows the 90 % similarity with Bacillus amyloliquefaciens,Lactobacillus jensenii 269-3 and Anoxybacillus sp.UARK-0.
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    MOLECULAR CHARACTERIZATION OF ENDOPHYTIC FUNGI (XYLERIASP)
    (Kongunadu Arts and Science College, CBE, 2018-08-07) Geetha M; Kanchana M
    Orchidaceae is one of the largest families with close to 25,000 species and encompassing 10% of flowering plants. Endophytic fungi are crucial, quantifiable and integral component of fungal biodiversity, and influenced by community diversity of plants and its structure. Fungal endophytes are also known for its role in ecological community by decreasing the range of environmental degradation, biodiversity loss, spoilage of water and land due to toxic insecticide, poisonous gases and industrial sewage. In this present study, the different Endophytic fungi like Fusarium sp.,Xylariasp., Westerdikellasp., Peniciilium sp. were isolated from two different orchid species Dendrobium sp. andBellaria sp. From four different isolates, xyleria sp. was selected for molecular identification. The genomic DNA was extracted from Xyleria sp. and which subjected to PCR using ITS 1 primer. The sequences were submitted in NCBI (National Centre for Biotechnological Information) for the analysis of homology.