Department of Botany

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Start date: 2000Subject: search.filters.subject.solubilization

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    MICROBIAL INOCULANTS-A BOON TO ZINC DEFICIENT CONSTRAINTS IN PLANTS –A REVIEW
    (International Journal of Scientific and Research Publications, 2014) K, Sunitha kumari; S N, Padma devi; S, Vasandha; S, Anitha
    Zinc is an essential micronutrient which plays a macro role in the growth and productivity of the plants. Zinc (Zn) deficiency hinders metabolic and physiological activity in plants due to its inevitable role as an enzyme cofactor. Many Indian soil exhibit Zn deficiency with the content much below the critical level of 1.5ppm. The conditions that make unavailability of zinc to plants are high pH, low organic matter content, high usage of P fertilizer, less textured soil and utilization of synthetic fertilizer to correct Zn deficiency which results in unavailability of zinc after seven days of application. An alternative eco-friendly approach to overcome Zn deficiency constraint in plants is by the application of microbial inoculants as a biofertilizer. Rhizospheric microorganisms play a vital role in the conversion of unavailable form of metal to available form through solubilization mechanism
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    ROLE OF PHOSPHOROUS SOLUBILIZING MICROORGANISMS TO ERADICATE P-DEFICIENCY IN PLANTS-A REVIEW
    (International Journal of Scientific and Research Publications, 2014) K, Nisha; S N, Padma Devi; S, Vasandha; K, Sunitha kumari
    Phosphorus is the second most important macro nutrient required by the plants for its optimum growth and yield. But it is considered to be a most limiting factor of many crop production systems, due to its unavailability of soluble forms in the soils. About 80% of applied P fertilizers are immobilized due to the formation of complex with Al or Fe in acidic soils or Ca in calcareous soils. An alternative way to circumvent P deficiency in soil and to improve crop production is through the utilization of microorganisms as biofertilizer. Rhizospheric microorganisms play a vital role in the transformation of unavailable form of P into available form which will be a boon to the agrarian communities to remove P deficiency in plants.
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    PHOSPHORUS SOLUBILIZING ACTIVITY OF MYCOBACTERIUM COSMETICUM UNDER VARIOUS CULTURAL CONDITIONS
    (International Journal of Advanced Scientific Research and Management, 2018) K, Sunitha kumari; S N, Padma Devi; K, Nisha
    Effect of various cultural conditions on the phosphorus solubilizing activity of Mycobacterium cosmeticum was tested under different parameters such as carbon (Glucose, Fructose, Sucrose, Maltose and Lactose), nitrogen (Ammonium sulphate, Sodium nitrate, Potassium nitrate and Urea), pH (5.0, 7.0 and 9.0) and temperature (25°C, 35°C and 45°C) using Pikovskaya’s medium amended with 0.5% tri calcium phosphate (TCP) as an insoluble source of P. Solubilization efficiency of TCP by M.cosmeticum varied with different carbon and nitrogen sources in both plate and broth assay. The results of plate assay revealed that P-solubilizing ability of the isolate was more in the presence of glucose (237.3%) as C-source and potassium nitrate (140.2%) as N-source. Whereas for the broth assay lactose was found to be the best (9.0 mg/l) and ammonium sulphate was the best nitrogen source (6.5mg/l). The isolate exhibited its maximum ability to solubilize TCP at the pH of 7.0 and at the incubation temperature of 35°C in both the assays. Thus the study confirmed the efficiency of M.cosmeticum to solubilize insoluble form of P under various cultural conditions and can be used as a source of Bioinoculants to eradicate P deficiency in plants and this study was the first to report P-solubilizing activity of M.cosmeticum.
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    ASSESSMENT OF COMPETENCE OF THE PSEUDOMONAS AERUGINOSA TO SOLUBILIZE INSOLUBLE FORM OF ZINC UNDER VARIOUS CULTURAL PARAMETERS
    (Springer, 2016) S N, Padma Devi; K, Sunitha kumari; S, Vasandha
    Efficiency of Pseudomonas aeruginosa to solubilize insoluble form of zinc to soluble form was studied under various cultural parameters such as carbon (Glucose, Fructose, Sucrose, Maltose and Lactose), nitrogen (Ammonium sulphate, Sodium nitrate, Potassium nitrate and Urea), pH (5.0, 7.0 and 9.0) and temperature (20°C, 30°C and 40°C). The experimental study was carried out by both qualitative (Plate assay) and quantitative (Broth assay) method. The Zn solubilizing efficiency of the isolate was found more when glucose was added as C-source in both plate (150%) and broth assay (16.62mg/l) and the efficiency was less in the medium amended with sucrose as carbon source in both qualitative (105.8%) and quantitative estimation (2.56mg/l). Among the four nitrogen sources Pseudomonas aeruginosa showed best solubilizing efficiency (262.5%) and solubilization of Zn (29.91mg/l) in the presence of Ammonium sulphate and recorded least solubilization efficiency in the presence of Sodium nitrate as nitrogen source in both plate (181.82%) and broth assay (21.92mg/l). It showed highest solubilization of zinc from ZnO at the incubation temperature of 30°C and the activity was less at the temperature of 40°C. pH 7 was the most favorable pH for solubilization and the efficacy was least when the pH reached its alkalinity. Thus the study confirmed the efficiency of Pseudomonas aeruginosa to solubilize insoluble form zinc and can be used as a source of bioinoculants to eradicate zinc deficiency in plants.