Department of Botany
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Item EXTRACTION OF AMPELOCISSUS LATIFOLIA AS A SOURCE OF ANTIBIOTIC(Muthayammal College of Arts & Science, Rasipuram, 2015-01-09) Sumathi R; Renuka Devi RThe Antifungal activity of different solvent extract of leaf and root powder of Amplelocissus latifolia was evaluated against Aspergillus niger and Penicillium candidum. The methanol, ethanol, hexane and acetone extract showed no zone of inhibition against both the organisms. The presence of protein was identified in phosphate buffer extract using Nin hydrin test and ELISA. The leaf and root extract contains 7.6ug/ul and 7.7ug/ul of protein respectively. The phytochemical studies showed the presence of Alkaloids, glycosides, carbohydrates, tannins and phenols in the leaf extract. The root extract contains only flavonoids . The FTIR analysis of root and leaf powder of Amplelocissus latifolia showed the presence of amines, amides alcohols carboxylic acids, nitric acids and phenolsItem ANTIFUNGAL, PHYTOCHEMICAL AND ANALYSIS OF STEM AND FRUIT OF AMPLELOCSSUS LATIFOLIA (ROXB)PLANCH(Kongunadu Arts and Science College, Coimbatore, 2018-08-07) Sumathi R; Kalaivani RAmpelocissus latifolia is climber belonging to the family Vitaceae and found throughout the India.Traditionally the plant has high medicinal value and used for indigenous treatment of numerousdiseases including bone fracture, dysentery, dental problems, stomach pain, body weakness, bodystrengthening etc. In present study freshly collected fruit and stem of Ampelocissus latifolia (Roxb.)were subjected to preliminary phytochemical and antifungal study. Different concentrations (10, 20,30, 40, 50μg/μl) of each extract were studied in the Enzyme Linked Immuno Sorbent Assay toquantify the proteins. Stem and fruit powder was examined by Fourier Transform Infra-Red Spectroscopy to recognize the functional groups. A wide range of phytochemical constituents have been isolated from this plant. A comprehensive account of phytochemical constituents and its antifungal activity reported are also included in this paper for exploring the immense medicinal potential of this plant.Item ANTIFUNGAL ACTIVITY OF AMPELOCISSUS LATIFOLIA(Govt. Arts College for Women, Salem, 2018-08-01) Sumathi R; Kalaivani RThe Antifungal activity of different solvent extract of stem and fruit powder of Amplelocissus latifolia was evaluated against Aspergillus niger and Penicillium candidum. The methanol, ethanol, hexane and acetone extract showed no zone of inhibition against both the organisms. The presence of protein was identified in phosphate buffer extract using Nin hydrin test and ELISA. The stem and fruit extract contains 7.6ug/ul and 7.7ug/ul of protein respectively. The phytochemical studies showed the presence of Alkaloids, glycosides, carbohydrates, tannins and phenols in the leaf extract. The root extract contains only flavonoids . The FTIR analysis of root and leaf powder of Amplelocissus latifolia showed the presence of amines, amides alcohols carboxylic acids, nitric acids and phenols.Item BIODEGRADATION OF POLYTHENE BAG USING BACTERIA ISOLATED FROM SOIL(Kongunadu Arts and Science College, Coimbatore, 2018-08-07) Jeeva Dharshni S; Kanchana MPlastic are one of the most widely used materials and in most cases, they are designed to have long life time. Though plastics have become cosmopolitan, now days, they are very hazardous and should be disposed properly. Land filling, incineration and recycling are the most common methods employed for the disposal of plastics with the side effects of environmental and health hazards. So, biodegradation will be the right choice for the disposal of plastic wastes. Soil samples from the compost yard have the rich consortia of biodegrading microbes. These samples were inoculated into nutrient agar medium with plastic as the sole carbon source for the isolation of the plastic degrading strains. Characterization of bacterial strains was done based on morphological, biochemical and molecular characterization. The process of biodegradation was observed at regular interval for 30 days in a synthetic medium containing LDPE films. The bacterial isolates,Actinomycetes sp, Bacillus mycoidesis, Bacillus subtilis,Brevundimonasdiminuta, Pseudomonas putidaandPseudomonas stutzeriwere able to reduce the weight of the polymer up to 17%,14%, 30%, 22% 29% and 25% respectively. Degradation was monitored by observing weight loss and changes in physical structure by Fourier Transform Infrared (FTIR) Spectroscopy