Department of Botany
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Item ESTIMATION OF PROTEIN AND CARBOHYDRATES IN DIFFERENT PULSES(PSGR Krishnammal College for Women, 2016-01-05) Ashiba A; Jeeva Lavanya S; Menachi A; Shifana Asmi M; Soundarya S; Vadivukkarasi K; Sumathi RPulses are essential for growth and metabolism of all living organisms. Leguminous crops are rich in protein and carbohydrates. The samples are soaked entire seed, seed coat, pulp, soaked water and also in boiled entire seed, seed coat, pulp and boiled water of black channa, white channa, fresh bean, dry bean, fresh pea and dry pea. The protein content was estimated by Bradford method and carbohydrate content was estimated by Anthrone method. The protein and carbohydrates are rich in boiled seeds than soaked seeds. The result shows the consumption of the boiled pulses is recommended and it is good for health.Item EXTRACTION OF AMPELOCISSUS LATIFOLIA AS A SOURCE OF ANTIBIOTIC(Muthayammal College of Arts & Science, Rasipuram, 2015-01-09) Sumathi R; Renuka Devi RThe Antifungal activity of different solvent extract of leaf and root powder of Amplelocissus latifolia was evaluated against Aspergillus niger and Penicillium candidum. The methanol, ethanol, hexane and acetone extract showed no zone of inhibition against both the organisms. The presence of protein was identified in phosphate buffer extract using Nin hydrin test and ELISA. The leaf and root extract contains 7.6ug/ul and 7.7ug/ul of protein respectively. The phytochemical studies showed the presence of Alkaloids, glycosides, carbohydrates, tannins and phenols in the leaf extract. The root extract contains only flavonoids . The FTIR analysis of root and leaf powder of Amplelocissus latifolia showed the presence of amines, amides alcohols carboxylic acids, nitric acids and phenolsItem OPTIMIZATION OF PROCESS PARAMETERS FOR LIPASE PRODUCTION BY ASPERGILLUS TERREUS USING COTTON SEED OIL AS SUBSTRATE(SNMV College of Arts and Science, Coimbatore, 2009-01-28) Sumathi R; Meerabai R SFungi isolated from soil where screened for exogenous lipolytic activity. The highest lipase activity was found in an isolate of Aspergillus terreus. Optimal cultural conditions influencing the growth and production of extra cellular lipase from this fungus was investigated. The lipase yield was maximum on day 5 of incubation, when the medium was supplemented with maltose and cotton seed oil as carbon source and potassium nitrate as nitrogen source, at pH 4 and at a temperature of 400 C. The possibility of using the lipase thus produced for transesterification of cotton seed oil to biodiesel was investigated.Item BIO-DIESEL – AN ALTERNATIVE FUEL(PSGR Krishnammal College for Women, Coimbatore, 2005-12-07) Sumathi R; Meerabai R SIndia is one of the countries in the world with billions of population and faces problems in this regard to the fuel requirement for increased transportation demand and now imports about 70% of its petroleum requirement. The current year consumption of diesel in India is approximately 40 million tons forming 40% of the total petroleum products consumption. The potential demand for biodiesel at 20% blend is estimated at 13.38 million tons by 2012. Indian oil corporation and Indian railways intend to use biodiesel in large quantities. The government is encouraging corporate and entrepreneurs to set-up biodiesel plants. Formers can develop and utilize waste lands and improve incomes, rural women labour will have more employment opportunities and soil fertility and the conditions will improveItem ANTIFUNGAL, PHYTOCHEMICAL AND ANALYSIS OF STEM AND FRUIT OF AMPLELOCSSUS LATIFOLIA (ROXB)PLANCH(Kongunadu Arts and Science College, Coimbatore, 2018-08-07) Sumathi R; Kalaivani RAmpelocissus latifolia is climber belonging to the family Vitaceae and found throughout the India.Traditionally the plant has high medicinal value and used for indigenous treatment of numerousdiseases including bone fracture, dysentery, dental problems, stomach pain, body weakness, bodystrengthening etc. In present study freshly collected fruit and stem of Ampelocissus latifolia (Roxb.)were subjected to preliminary phytochemical and antifungal study. Different concentrations (10, 20,30, 40, 50μg/μl) of each extract were studied in the Enzyme Linked Immuno Sorbent Assay toquantify the proteins. Stem and fruit powder was examined by Fourier Transform Infra-Red Spectroscopy to recognize the functional groups. A wide range of phytochemical constituents have been isolated from this plant. A comprehensive account of phytochemical constituents and its antifungal activity reported are also included in this paper for exploring the immense medicinal potential of this plant.Item ANTIFUNGAL ACTIVITY OF AMPELOCISSUS LATIFOLIA(Govt. Arts College for Women, Salem, 2018-08-01) Sumathi R; Kalaivani RThe Antifungal activity of different solvent extract of stem and fruit powder of Amplelocissus latifolia was evaluated against Aspergillus niger and Penicillium candidum. The methanol, ethanol, hexane and acetone extract showed no zone of inhibition against both the organisms. The presence of protein was identified in phosphate buffer extract using Nin hydrin test and ELISA. The stem and fruit extract contains 7.6ug/ul and 7.7ug/ul of protein respectively. The phytochemical studies showed the presence of Alkaloids, glycosides, carbohydrates, tannins and phenols in the leaf extract. The root extract contains only flavonoids . The FTIR analysis of root and leaf powder of Amplelocissus latifolia showed the presence of amines, amides alcohols carboxylic acids, nitric acids and phenols.Item PHYSIO-CHEMICAL ANALYSIS OF PAPER MILL EFFLUENT, DYEING MILL EFFLUENT AND SUGAR MILL EFFLUENT(Sri Ramakrishna College of Arts and Science for Women, 2018-02-16) Logeshwari J; Masilamani S; Nivedha Nandhini I K R; Poomathi S; Yamuna G; Sumathi RNature, now a days is suffering from a serious problem of environmental pollution. Increased industrialization has resulted in indiscriminate release of toxic waste into the surrounding environment. Industrial effluent are the major pollution sources mainly in water pollution. Sugar industry is a seasonal industry operating for maximum of 4-5 month in one season. The effluent discharge from sugar industry consist of a number of organic and heavy metal pollutant in dissolved or suspended form that can bring about changes in the physical, chemical and physiological sphere of the biota. Textile industries are large industrial consumers of water as well as producers of waste water. The effluents are rich in dyes and chemicals, some of which are non-biodegradable and carcinogenic and pose a major threat to environment. These industries showed high concentrations of EC, TS, TSS, TDS, BOD, COD, alkalinity, total hardness etc., when compared to BIS, where paper and pulp industry showed high contaminants when compared ti dyeing and sugar industry.Item ESTIMATION OF PROTEIN AND CARBOHYDRATES IN DIFFERENT CEREALES(KSG College of Arts& Science, Coimbatore, 2017-09-26) Sumathi RCereals are essential for growth and metabolism of all living organisms. Cereals are rich in protein and carbohydrates. The samples are soaked entire seed, seed coat, pulp, soaked water and also in boiled entire seed, seed coat, pulp and boiled water of green gram, Bengal gram, black urad and white urad. The protein content was estimated by Bradford method and carbohydrate content was estimated by Anthrone method. The protein and carbohydrates are rich in boiled seeds than soaked seeds. The result shows the consumption of the boiled pulses is recommended and it is good for healthItem ANTIFUNGAL, PHYTOCHEMICAL, PROTEIN AND FT-IR ANALYSIS OF AMPELOCISSUS LATIFOLIA (ROXB.) PLANCH(International Journal of Pharmacy and Biological Sciences, 2018) Kalaivani V; Sumathi RIn recent times, focus on plant research has increased all over the world. The therapeutic effect of these plants for the treatment of various diseases is based on the chemical constituents present in them. Medicinal plants provide affordable means of health care for poor and marginalised people. Ampelocissus is a genus of Vitaceae family. The FT-IR analysis of stem and fruit powder showed the alkanes, alkenes, alcohols, esters, amines, ketones, aldehydes. Despite its well- recognised medicinal and economic potential, there are no commercial plantations worldwide. Wild plants have continuously been used to meet the growing commercial demand in terms of their socio-economic value. Ampelocissus latifolia is the plant which may not be freely available in future due to over exploitation, habitat destruction or lack of domestication and cultivation. Since this plant species is an important ingredient of several medicines due to its usefulness, phytochemical investigation for isolation of important active ingredients through cell culture will be helpfulItem BIODIESEL PRODUCTION FROM COTTON SEED OIL USING EXTRACELLULAR LIPASE ENZYME AS CATALYST(Advances in Plant Sciences, 2013) Sumathi R; Meerabai R SBiodiesel (Fatty acid methyl esters), which is derived from triglycerides by transesterification with methanol, has attracted considerable attention during the past decade as a renewable, biodegradable and nontoxic fuel. Several processes for biodiesel fuel production have been developed among which the enzymatic process offers several advantages than the chemical routes. Enzymatic transestrification using lipase has become more attractive for biodiesel fuel production, since the glycerol produced as a by product can be easily recovered and the purification of fatty acid methyl ester is simple to accomplish. The main hurdle to the commercialization of this system is the cost of lipase production. As a means of reducing the cost, the extra cellular enzyme is significantly advantageous. In the present investigation, optimization of process parameters for high lipase production by the microbes viz, Mucor racemosus, Aspergillus terreus and Penicillium candidum using SOB and MYGP medium were carried out. Culture filtrates were used as extra cellular crude enzyme source as a catalyst for conversion of cotton seed oil to biodiesel and the parameters such as quantity of enzyme and methanol needed for significant yield of biodiesel were standardized