Department of Botany

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Author: search.filters.author.Sujithra V

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    SCREENING AND IDENTIFICATION OF IAA PRODUCING BACTERIA
    (St.Xavier’s College, 2019-11-28) Sujithra V; Kanchana M
    Biofertilizers are the substances which make use of microorganisms to fertile the soil. The plant growth promoting bacteria was act as biofertilizer to enhance the plant growth. These types of microorganism are used to increase the level of nutrients in the plants. They let the plants into a healthy environment and do not cause the pollution. Seven bacteria strains isolated from rhizospheric soil of Spathaglottisplicata.The screening and isolation of plant growth promoting bacteria was subject to confirm through the Salkowshi reagent. The pink color indicated the presence of IAA in plant growth promoting bacteria. Out of 7 bacterial isolates 3 bacterial cultures were produced high amount of auxin content. The plant growth promoting bacteria were identified by morphological analysis [gram stain, cell shape, size and motility] and biochemical analysis [IMVIC Tests].
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    SCREENING OF PLANT GROWTH PROMOTING BACTERIA FROM RHIZOSPHERIC SOIL
    (Rathinam College of Arts and Sciemce, 2019-02-27) Sujithra V; Kanchana M
    A major focus on plant growth-promoting microbes (PGPM) for restoring the agro ecosystems to their original shape is gaining the attention of agronomists and environmentalists. Increase in cost of fertilizers and worldwide energy crises, low purchasing power of farmers, increase in cost of production restricted the use of chemical fertilizers alone as a source of plant nutrient. Work on rhizospheric bacteria has already shown potential in the management of various agricultural problems, and especially their use in the form of biofertilizers and biopesticides has resulted in meager reliance on synthetic agrochemicals. Therefore, the present investigation started with the bacterial isolation from rhizosphere soil of orchid root. After evaluation for their plant growth promoting attributes, were found potential strain for Plant Growth Promoting activity as production of IAA. The screening of plant growth promoting bacteria was subjected to confirm through color change of yellow to pink color in the salkowshi reagent. The bacteria were efficiently produced auxin in its fifth day of growth. The shaking conditions of the bacteria in Nutrient broth produced more auxin content than static conditions
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    ISOLATION AND MOLECULAR CHARACTERIZATION OF PLANT GROWTH PROMOTING FUNGI FROM RHIZOSPHERIC SOIL
    (RVS Siddha Medical college and Hospital, 2018-12-07) Sujithra V; Geetha M; Jeeva dharshni S; Kanchana M
    The present study is focused on identification and characterization of phosphate solubilizing microorganisms. Phosphate solubilization by microbes in soil is an important process for the abundance of phosphate in available format helps in eradication of phosphate deficiency in plants. The dissolution of inorganic phosphate by microbial communities including fungi is though common under in vitro conditions; the performance of phosphate solubilizing microbes in situ has been contradictory. Therefore, acquiring insightful knowledge in plant associated organisms may help in solubilization of mineral phosphorous for easy uptake by the plants. Fungi have the ability to solubilize phosphorous by production of organic acids and are known to have a higher efficiency of solubilization than bacteria. A sterile rhizospheric fungus was isolated from rhizospheric soil of spathoglottisplicata and was found to be dominant with highest phosphate solubilization capacity. The fungus is identified through its morphological features and molecular characterization by partial rDNA-ITS sequence analysis. Based on BLAST search analysis the fungus was found closest homolog to humiculasp, with maximum identity of 98%.
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    GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM NYMPHAEA ODORATAROOT EXTRACT AND ITS ANTIBACTERIAL ACTIVITY
    (K.S.G. College of Arts and Science, 2018-08-11) Sujithra V; Geetha M; Jeevadharshni S; Kanchana M
    The biosynthesis of nanoparticles has been proposed as a cost-effective and environmentally friendly alternative to chemical and physical methods. Plant-mediated synthesis of nanoparticles is a green chemistry approach that connects nanotechnology with plants. Various techniques used to characterize synthesized nanoparticles are SEM and UV–Visible spectrophotometer. UV–Visible spectrophotometer showed absorbance peak in range of 450–550nm. The silver nanoparticles showed antibacterial activities of Nymphaea odorataroot extract against both gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) microorganisms. The synthesized (silverNps) were found to be effective as antimicrobial agents against some important human pathogens
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    GREEN SYNTHESIS OF SILVER NANOPARTICLES USING MESUAFERREALINN FLOWER BUDS AND EVALUTION OF THEIR ANTI MICROBIAL ACTIVITIES
    (KSG college of Arts and Science, Coimbatore., 2018-08-11) Jeeva Dharshni S; Gomathi S; Sujithra V; Geetha M; Kanchana M
    Green synthesis of nano particles is an emerging branch of nano technology. There is an increasing commercial demand for nano particles due to their wide applicability in various areas, such as electronics, catalysis, chemistry, energy and medicine. Metallic nano particles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. In contrast the current study describes a cost effective and environment friendly technique for green synthesis of silver nano particles from 1mM. AgNo3 solution through the extract of species flower bud. Nano particles were characterized by using UV-Vis absorption spectroscopy, SEM analysis and elementary analysis, showed the average particle size of 46nm-101nm in chloroform extract and 37nm-66nm in ethanol extract as well as revealed their spherical and cubic structures respectively. Biologically synthesized nanoparticles were found to be highly antimicrobial against different multidrug resistant human pathogens
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    GREEN SYNTHESIS AND PHYTOCHEMICAL ANALYSIS OF SILVER NANO PARTICLES USING ROOT OF NELUMBO NUCIFERA AND THEIR ANTIBACTERIAL ACTIVITY
    (KSG College of Arts and Science, CBE., 2018-08-11) Geetha M; Muthumeenachi; Jeevadharshni S; Sujithra V; Kanchana M
    In the present study, biochemical analysis of Acalyphaindica,biosynthesis of silver nanoparticles and its antimicrobial activity on clinical laboratory bacterial pathogens were investigated. Silver nanoparticles were rapidly synthesized using root extract of Acalyphaindica and the formation of nanoparticles was observed. The results recorded from UV-vis spectrum, and scanning electron microscopy (SEM) support the biosynthesis and characterization of silver nanoparticles. Further, the antibacterial activity of synthesized silver nanoparticles showed effective inhibitory activity against clinical laboratory pathogens Viz., Escherichia coli (gram negative) and Staphylococcus aureus (gram positive).
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    ISOLATION AND MOLECULAR CHARACTERIZATION OF PHOSPHATE SOLUBILIZING FUNGI FROM RHIZOSPHERIC SOIL
    (International Journal of Pharmacy and Biological Sciences-IJPBS, 2019-04-01) Sujithra V; Sankavi M; Nathiya S; Sreenithi H; Aarthika B; Mahalakshmi C; Geetha M; Jeevadharshni S; Kanchana M
    Aim: Phosphorus is abundant in soil in both organic and inorganic forms; nevertheless, it is unavailable to plants. Accordingly, soil becomes phosphorus deficient, making phosphate one of the most important nutrient elements limiting crop productivity. To prevent the phosphate deficiency, phosphate solubilizing microorganisms could play an important role in making phosphate available for plants by dissolving insoluble Phosphate. Materials and methods: The serial dilution was done in rhizospheric soil and humicola.sp, fungi was isolated. The morphological and molecular characters were studied. Using pikovskaya media humicolafungi was identified to be phosphate solubilizing. This fungus helps to promote plant growth. Result: Phosphate in the soil is essentially unavailable to plants and use of plant associated organisms may help in solubilization of mineral phosphorous for easy uptake by the plants. Fungi have the ability to solubilize phosphorous by production of organic acids and are known to have a higher efficiency of solubilization than bacteria. Increase in cost of fertilizers and worldwide energy crises, low purchasing power of farmers, increase in cost of production restricted the use of chemical fertilizers alone as a source of plant nutrient. Under such condition it has become alternative to use all available resources of plant nutrients including microorganisms like Phosphate Solubilizing Microorganisms for sustainable soil fertility and productivity. A sterile rhizospheric fungus was isolated from rhizospheric soil of spathoglottisplicataand was found to be dominant with highest phosphate solubilization capacity. The fungus could identify by morphological features, molecular characterization was carried was found closest homolog to humicolasp, with maximum identity of 98%. Conclusion: The humicolais found to be in symbiotic association with the plant. It can be used as a substitute for chemical fertilizer. Therefore preserves the soil fertility.