Department of Botany
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Item OPTIMIZATION OF PROCESS PARAMETERS FOR LIPASE PRODUCTION BY ASPERGILLUS TERREUS USING COTTON SEED OIL AS SUBSTRATE(SNMV College of Arts and Science, Coimbatore, 2009-01-28) Sumathi R; Meerabai R SFungi isolated from soil where screened for exogenous lipolytic activity. The highest lipase activity was found in an isolate of Aspergillus terreus. Optimal cultural conditions influencing the growth and production of extra cellular lipase from this fungus was investigated. The lipase yield was maximum on day 5 of incubation, when the medium was supplemented with maltose and cotton seed oil as carbon source and potassium nitrate as nitrogen source, at pH 4 and at a temperature of 400 C. The possibility of using the lipase thus produced for transesterification of cotton seed oil to biodiesel was investigated.Item BIO-DIESEL – AN ALTERNATIVE FUEL(PSGR Krishnammal College for Women, Coimbatore, 2005-12-07) Sumathi R; Meerabai R SIndia is one of the countries in the world with billions of population and faces problems in this regard to the fuel requirement for increased transportation demand and now imports about 70% of its petroleum requirement. The current year consumption of diesel in India is approximately 40 million tons forming 40% of the total petroleum products consumption. The potential demand for biodiesel at 20% blend is estimated at 13.38 million tons by 2012. Indian oil corporation and Indian railways intend to use biodiesel in large quantities. The government is encouraging corporate and entrepreneurs to set-up biodiesel plants. Formers can develop and utilize waste lands and improve incomes, rural women labour will have more employment opportunities and soil fertility and the conditions will improveItem BIODIESEL PRODUCTION BY ALKALI CATALYST USING PONGAMIA SEED OIL AND ITS CHARACTERIZATION.(Asian Academic Research Journal of Multidisciplinary, 2014) Sumathi, R; Meerabai R SBiodiesel production has received considerable attention in the past as a biodegradable and non-polluting fuel. The production of biodiesel by transesterification process employing alkali catalyst has been widely accepted for its high conversion and reaction rates. In India, non edible oils like jatropha oil, rubber seed oil, madhuca oil etc., are available in abundance, which can be converted to biodiesel. In the present study, biodiesel has been prepared from pongamia seed oil. As the acid value of this oil is high, it is reduced by esterification followed by transesterification process. The experimental work revealed the suitability of Sodium hydroxide / Potassium hydroxide and methanol as solvent for maximum methanolysis at 60-70oC in 45 minutes. Properties of biodiesel are in concordance with international standardsItem BIODIESEL PRODUCTION FROM COTTON SEED OIL USING EXTRACELLULAR LIPASE ENZYME AS CATALYST(Advances in Plant Sciences, 2013) Sumathi R; Meerabai R SBiodiesel (Fatty acid methyl esters), which is derived from triglycerides by transesterification with methanol, has attracted considerable attention during the past decade as a renewable, biodegradable and nontoxic fuel. Several processes for biodiesel fuel production have been developed among which the enzymatic process offers several advantages than the chemical routes. Enzymatic transestrification using lipase has become more attractive for biodiesel fuel production, since the glycerol produced as a by product can be easily recovered and the purification of fatty acid methyl ester is simple to accomplish. The main hurdle to the commercialization of this system is the cost of lipase production. As a means of reducing the cost, the extra cellular enzyme is significantly advantageous. In the present investigation, optimization of process parameters for high lipase production by the microbes viz, Mucor racemosus, Aspergillus terreus and Penicillium candidum using SOB and MYGP medium were carried out. Culture filtrates were used as extra cellular crude enzyme source as a catalyst for conversion of cotton seed oil to biodiesel and the parameters such as quantity of enzyme and methanol needed for significant yield of biodiesel were standardizedItem BIODIESEL PRODUCTION FROM COTTON SEED OIL USING ALKALI CATALYST AND ITS CHARACTERIZATION(Asian Journal of Bio Science, 2012) Sumathi R; Meerabai R SBiodiesel production has received considerable attention in the past as a biodegradable and non-polluting fuel. The production of biodiesel by transesterification process employing alkali catalyst has been widely accepted for its high conversion and reaction rates. In India, edible oils like sunflower, mustard, palm, cotton seed oil, groundnut oil etc., are available in abundance, which can be converted to biodiesel. In the present study, biodiesel has been prepared from cotton seed oil. As the acid value is less than two, it is easily converted to biodiesel by transesterification process. The experimental work revealed the suitability of sodium hydroxide/potassium hydroxide and methanol as solvent for maximum methanolysis at 60-70°C in 45 minutes. Properties of biodiesel are in concordance with international standards.Item LIPASE PRODUCTION BY ASPERGILLUS TERREUS USING COTTON SEED OIL AS CARBON SOURCE(Plant Archives, 2012) Sumathi R; Meerabai R SFungi isolated from soil were screened for exogenous lipolytic activity. The highest lipase activity was found in an isolate of Aspergillus terreus. Optimal cultural conditions influencing the growth and production of extra cellular lipase from this fungus was investigated. The lipase yield was maximum on day 5 of incubation when the medium was supplemented with maltose and cotton seed oil as sole carbon source and potassium nitrate as nitrogen source at pH 7 and at temperature of 40°C