Department of Botany
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Item SCREENING OF PLANT GROWTH PROMOTING BACTERIA FROM RHIZOSPHERIC SOIL(Rathinam College of Arts and Sciemce, 2019-02-27) Sujithra V; Kanchana MA major focus on plant growth-promoting microbes (PGPM) for restoring the agro ecosystems to their original shape is gaining the attention of agronomists and environmentalists. Increase in cost of fertilizers and worldwide energy crises, low purchasing power of farmers, increase in cost of production restricted the use of chemical fertilizers alone as a source of plant nutrient. Work on rhizospheric bacteria has already shown potential in the management of various agricultural problems, and especially their use in the form of biofertilizers and biopesticides has resulted in meager reliance on synthetic agrochemicals. Therefore, the present investigation started with the bacterial isolation from rhizosphere soil of orchid root. After evaluation for their plant growth promoting attributes, were found potential strain for Plant Growth Promoting activity as production of IAA. The screening of plant growth promoting bacteria was subjected to confirm through color change of yellow to pink color in the salkowshi reagent. The bacteria were efficiently produced auxin in its fifth day of growth. The shaking conditions of the bacteria in Nutrient broth produced more auxin content than static conditionsItem ISOLATION AND MOLECULAR CHARACTERIZATION OF PLANT GROWTH PROMOTING FUNGI FROM RHIZOSPHERIC SOIL(RVS Siddha Medical college and Hospital, 2018-12-07) Sujithra V; Geetha M; Jeeva dharshni S; Kanchana MThe present study is focused on identification and characterization of phosphate solubilizing microorganisms. Phosphate solubilization by microbes in soil is an important process for the abundance of phosphate in available format helps in eradication of phosphate deficiency in plants. The dissolution of inorganic phosphate by microbial communities including fungi is though common under in vitro conditions; the performance of phosphate solubilizing microbes in situ has been contradictory. Therefore, acquiring insightful knowledge in plant associated organisms may help in solubilization of mineral phosphorous for easy uptake by the plants. Fungi have the ability to solubilize phosphorous by production of organic acids and are known to have a higher efficiency of solubilization than bacteria. A sterile rhizospheric fungus was isolated from rhizospheric soil of spathoglottisplicata and was found to be dominant with highest phosphate solubilization capacity. The fungus is identified through its morphological features and molecular characterization by partial rDNA-ITS sequence analysis. Based on BLAST search analysis the fungus was found closest homolog to humiculasp, with maximum identity of 98%.Item ISOLATION AND MOLECULAR CHARACTERIZATION OF PHOSPHATE SOLUBILIZING FUNGI FROM RHIZOSPHERIC SOIL(International Journal of Pharmacy and Biological Sciences-IJPBS, 2019-04-01) Sujithra V; Sankavi M; Nathiya S; Sreenithi H; Aarthika B; Mahalakshmi C; Geetha M; Jeevadharshni S; Kanchana MAim: Phosphorus is abundant in soil in both organic and inorganic forms; nevertheless, it is unavailable to plants. Accordingly, soil becomes phosphorus deficient, making phosphate one of the most important nutrient elements limiting crop productivity. To prevent the phosphate deficiency, phosphate solubilizing microorganisms could play an important role in making phosphate available for plants by dissolving insoluble Phosphate. Materials and methods: The serial dilution was done in rhizospheric soil and humicola.sp, fungi was isolated. The morphological and molecular characters were studied. Using pikovskaya media humicolafungi was identified to be phosphate solubilizing. This fungus helps to promote plant growth. Result: Phosphate in the soil is essentially unavailable to plants and use of plant associated organisms may help in solubilization of mineral phosphorous for easy uptake by the plants. Fungi have the ability to solubilize phosphorous by production of organic acids and are known to have a higher efficiency of solubilization than bacteria. Increase in cost of fertilizers and worldwide energy crises, low purchasing power of farmers, increase in cost of production restricted the use of chemical fertilizers alone as a source of plant nutrient. Under such condition it has become alternative to use all available resources of plant nutrients including microorganisms like Phosphate Solubilizing Microorganisms for sustainable soil fertility and productivity. A sterile rhizospheric fungus was isolated from rhizospheric soil of spathoglottisplicataand was found to be dominant with highest phosphate solubilization capacity. The fungus could identify by morphological features, molecular characterization was carried was found closest homolog to humicolasp, with maximum identity of 98%. Conclusion: The humicolais found to be in symbiotic association with the plant. It can be used as a substitute for chemical fertilizer. Therefore preserves the soil fertility.