Department of Botany
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Item MICROBIAL DEGRADATION OF LOW-DENSITY POLYETHYLENE (LDPE) BY FUNGUS ISOLATED FROM LANDFILL SOIL(Avinashilingam institute for home science and higher education, Coimbatore, 2019-12-18) Jeeva Dharshni S; Kanchana MPlastics are natural or synthetic represent the class of polymeric substances. A stable increase in the use of plastic products has accelerated the pollution. Several attempts have been made to control the problem at large by resorting to both chemical and biological methods. Eradication of pollution by chemical methods leads to many side effects such as, release of toxic chemicals and gases into the atmosphere. Therefore an alternative method must be identified and it should be eco-friendly manner. Hence a current study is focused on identify the biological method for the degradation of plastics without any side effects. Low density polyethylene polymer can be degraded by microorganisms or enzymes by means of cutting down the molecular chains. A low density polyethylene (LDPE) is one of the hazardous polymers used for the present study and makes it degrade fully in a rapid manner. There are two fungal strains Aspergillus sp. and Xylaria sp. were identified for their high degradable activity. After 30 days of incubation the efficacy of fungal isolates for their polymer degradation was determined through various parameters such as pH variation, Optical Density of the culture and estimation of CO2 during degradation process.Item BIODEGRADATION OF LOW DENSITY POLYETHYLENE (LDPE) FILMS USING BACTERIA ISOLATED FROM PLASTIC DUMPED SOIL(St. Xavier’s College (Autonomous), Palayamkottai, 2019-11-28) Jeeva Dharshni S; Kanchana MPlastic are one of the most widely used materials and in most cases, they are designed to have long life time. Though plastics have become cosmopolitan, now days, they are very hazardous and should be disposed off, properly. Land filling, incineration and recycling are the most common methods employed for the disposal of plastics and all methods have their own environmental and health hazards. So, biodegradation will be the right choice for the proper disposal of plastic wastes. Soil samples from the compost yard have the rich consortia of biodegrading microbes. These samples were inoculated into nutrient agar medium with plastic as the sole carbon source for the isolation of the plastic degrading strains. Characterization of bacterial strains was done based on morphological, biochemical and molecular characterization. The process of biodegradation was observed at regular interval for 90 days in a synthetic medium containing Low Density Polyethylene (LDPE) films. The bacterial isolates,Bacillus spandPseudomonas spwere able to reduce the weight of the polymer up to 0.3% and 0.6% respectively. Degradation was monitored by observing weight loss and changes in physical structure by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) Spectroscopy.Item ISOLATION AND DEGRADATION OF MONOSTAR INSECTICIDE DEGRADING BACTERIA FROM AGRICULTURAL SOIL AND ITS GROWTH RESPONSE(Kongunadu Arts and Science College, Coimbatore, 2017-10-09) Jeeva Dharshni S; Kanchana MGeneral agricultural use of pesticides carries with it potential hazards to man and directly by exposure to toxic residues in food and indirectly to the environment. An effort is undertaken in the present study for developing active microbial strains that could be of relevant in bioremediation of pesticides contaminated soil. The bacterial isolates were screened through biochemical and microbial analysis from the soil of agricultural land in Omalur region, Tamil nadu. An efficient strain having organophosphorus - Monostar insecticide (monocrotophos) degrading ability was isolated and identified on the basis of 16S rDNA sequence analysis as Bacillus thuringiensis. The isolated sequences were submitted in NCBI (national centre for biotechnological information) for the analysis of homology. As a result the Bacillus thuringiensis sequences of BLAST shows the 100 % similarity with Bacillus cereusItem GREEN SYNTHESIS OF SILVER NANOPARTICLES USING MESUAFERREALINN FLOWER BUDS AND EVALUTION OF THEIR ANTI MICROBIAL ACTIVITIES(KSG college of Arts and Science, Coimbatore., 2018-08-11) Jeeva Dharshni S; Gomathi S; Sujithra V; Geetha M; Kanchana MGreen synthesis of nano particles is an emerging branch of nano technology. There is an increasing commercial demand for nano particles due to their wide applicability in various areas, such as electronics, catalysis, chemistry, energy and medicine. Metallic nano particles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. In contrast the current study describes a cost effective and environment friendly technique for green synthesis of silver nano particles from 1mM. AgNo3 solution through the extract of species flower bud. Nano particles were characterized by using UV-Vis absorption spectroscopy, SEM analysis and elementary analysis, showed the average particle size of 46nm-101nm in chloroform extract and 37nm-66nm in ethanol extract as well as revealed their spherical and cubic structures respectively. Biologically synthesized nanoparticles were found to be highly antimicrobial against different multidrug resistant human pathogensItem BIODEGRADATION OF POLYTHENE BAG USING BACTERIA ISOLATED FROM SOIL(Kongunadu Arts and Science College, Coimbatore, 2018-08-07) Jeeva Dharshni S; Kanchana MPlastic are one of the most widely used materials and in most cases, they are designed to have long life time. Though plastics have become cosmopolitan, now days, they are very hazardous and should be disposed properly. Land filling, incineration and recycling are the most common methods employed for the disposal of plastics with the side effects of environmental and health hazards. So, biodegradation will be the right choice for the disposal of plastic wastes. Soil samples from the compost yard have the rich consortia of biodegrading microbes. These samples were inoculated into nutrient agar medium with plastic as the sole carbon source for the isolation of the plastic degrading strains. Characterization of bacterial strains was done based on morphological, biochemical and molecular characterization. The process of biodegradation was observed at regular interval for 30 days in a synthetic medium containing LDPE films. The bacterial isolates,Actinomycetes sp, Bacillus mycoidesis, Bacillus subtilis,Brevundimonasdiminuta, Pseudomonas putidaandPseudomonas stutzeriwere able to reduce the weight of the polymer up to 17%,14%, 30%, 22% 29% and 25% respectively. Degradation was monitored by observing weight loss and changes in physical structure by Fourier Transform Infrared (FTIR) Spectroscopy