Department of Botany

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Author: search.filters.author.Agnes Nirmala K

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    LEUCAS ASPERA – A REVIEW OF ITS BIOLOGICAL ACTIVITY
    (Systematic Review in Pharmacy, 2018-01-08) Agnes Nirmala K; Kanchana M
    Herbal plants are natural source of phytochemicals and widely used in Pharmacetical, food and cosmetic industries. Leucas aspera Linn., commonly known as Thumbai or Dronpushpi is a small erect, branched annual aromatic herb , distributed throughout India from the Himalayas down to Ceylon. Medicinally, it has been proven to possess various pharmacological values like antifungal, antipyretic, antioxidant, antimicrobial, antinociceptive, analgesic, anti-diarrheal, insecticidal, antiinflammatory, analgesic and cytotoxic activity. The Study on various biological properties of this plant will be useful for its potential use in respective industries. The available reports on anti microbial activity, anti oxidant activity and pharmacological value of L. aspera is explained in this review.
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    EFFECT OF SALT STRESS ON TOTAL CHLOROPHYLL CONTENT IN LEUCAS ASPERA
    (MacMillan New Delhi, 2017) Agnes Nirmala K; Kanchana M
    Leucas aspera is well known for its traditional uses as antipyretic and insecticide. It is commonly known as “Thumbai” and distributed throughout India from the Himalayas down to Srilanka. This plant possesses immense medicinal properties such as antifungal, antioxidant, anti-inflammatory, antimicrobial, antinociceptive and cytotoxic activity. Soil salinity is a very important issue affecting agriculture and over 800 million hectares of the world land are affected by salt stress. As Leucas aspera plant distributed throughout India, study on salinity tolerance of this plant may give more insights on abiotic stress research. The effect on photosynthesis can be gauged from the effect on the photosynthetic pigments and salinity stress significantly affected the chlorophyll content. In this study, the effect of salt stress on total chlorophyll content was analysed. The plant was treated with 150 mM NaCl and total chlorophyll content was estimated at 0 hrs, 12 hrs, 24 hrs, 48 hrs, 72 hrs and 120 hrs. We also estimated total chlorophyll content after three days with different salt treatments ranging 50 mM, 100 mM, 150 mM, 200 mM, 250 mM and 300 mM. The chlorophyll content significantly reduced after 72 hrs of 150 mM and higher salt concentrations. The chlorophyll content was not significantly affected up to 48 hrs in all salt concentrations analysed.
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    IDENTIFICATION OF ACTIN GENE HOMOLOGUS FROM LEUCAS ASPERA
    (Hindustan College of Arts and Science, Coimbatore, 2015-02-25) Agnes Nirmala K; Kanchana M; Saranya M; UmaMaheswari P
    Leucas aspera commonly known as ‘Thumbai’ is used traditionally as an antipyretic and insecticide. It is distributed throughout India from the Himalayas down to Ceylon and possess various pharmacological activities like antifungal, antioxidant, antimicrobial, antinociceptive and cytotoxic activity. As this plant distributed throughout India, analysis of genes induced during abiotic stress may give better insights on abiotic stress tolerance. Quantitative real time RT-PCR is a better technique to analyse the differential expression of genes. A major step in gene quantification analysis is data normalisation in real-time RT-PCR . Normalizing through reference genes, or housekeeping genes, can make more accurate and reliable results from reverse transcription real-time quantitative polymerase chain reaction (qPCR). The classical housekeeping genes involved in basic cellular processes such as 18 S rRNA, ubiquitin, actin, b-tubulin, and glyceraldehyde-3-phosphate dehydrogenase have been recurrently used as internal controls for gene expression analysis in plant as they are supposed to have a uniform expression in all samples and experimental conditions tested. To analyse the differential expression of genes involved in abiotic stress, Actin gene homologue was identified from Leucas aspera to use as house keeping genein this experiment. Complete coding region (CDS) of actin gene from different plants were downloaded from NCBI database. Conserved region of the Actin gene was deduced and degenerate primers were designed using bioinformatics tools ClustalW and PriFi. Using the degenerate primers, partial gene homologue of actin from Leucas aspera genomic DNA was amplified and the amplicon was sequenced. The sequence data was annotated for exons and introns were submitted in NCBI database. This gene will be used as housekeeping gene for normalization in differential expression studies in this plant.