Department of Botany

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GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN ETHANOLIC STEM BARK EXTRACT OF AN ENDEMIC PLANT CHIONANTHUS MALA-ELENGI (DENNST.) P.S. GREEN (OLEACEAE) (PDF)
(EBSCO, 2020-04) Malathi K; Krishnaveni C; Sahithya S; Akila Shree J
Traditional and herbal medicines are the most easily available health care remedies. Chionanthus mala-elengi (Dennst.) P. S. Green is an endemic tree species of the family Oleaceae. It is used in giddiness, epilepsy, wound healing and liver disease. Phytochemical studies of this plant have not been reported so far. The aim of the present study is to characterize the chemical constituents present in ethanolic stem bark extract of Chionanthus mala-elengi. Thirty grams of powdered stem bark was extracted using 150 ml ethanol in Soxhlet apparatus. The mixture was filtered. The filtrates were evaporated and the dried ethanolic stem bark extract was used for further studies. Gas Chromatography - Mass Spectrometry analysis provided different peaks indicating the presence of sixteen different phytochemical compounds. Thus, the qualitative determination of stem bark ethanolic extract of C. mala-elengi using GC-MS analysis revealed the presence of various bioactive compounds which is used for different ailments by traditional practitioners.
ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF ENDOPHYTIC FUNGI FROM THE LEAVES OF COELOGYNE SPECIES, AND THEIR ROLE AS AN ANTIMICROBIAL AGENT
(Research Journal of Pharmacy and Technology, 2021) Akila Shree J; Krishnaveni C
Endophytic fungi from Orchids believed to have an essential symbiotic relationship with the plant for both the germination of seeds and the development of young plantlets. Endophytes are microorganisms which live in the intercellular spaces of healthy host tissues without causing apparent symptoms. Endophytic fungi isolated from the medicinal plants are used for the development of drugs as they act as the source of bioactive compounds. This study has been designed to isolate the fungal endophytes from the leaves of three epiphytic orchid species (Coelogyne nervosa, A. Rich., Coelogyne mossiae, Rolf and Coelogyne cristata, Lindl) from the same genus Coelogyne, collected from Ooty flora, Coonoor. About five different endophytes were identified and their morphological characters were studied. Coelogyne cristata showed maximum colonization frequency. Among the five species, Fusarium species showed antibacterial activity against the gram-positive bacteria Bacillus Subtilis, a pathogen found in soil, water and food. Fungal genomic DNA isolated for molecular identification from the four fungal species.