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Author: search.filters.author.Kanchana MHas files: Yes

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    GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM NYMPHAEA ODORATAROOT EXTRACT AND ITS ANTIBACTERIAL ACTIVITY
    (K.S.G. College of Arts and Science, 2018-08-11) Sujithra V; Geetha M; Jeevadharshni S; Kanchana M
    The biosynthesis of nanoparticles has been proposed as a cost-effective and environmentally friendly alternative to chemical and physical methods. Plant-mediated synthesis of nanoparticles is a green chemistry approach that connects nanotechnology with plants. Various techniques used to characterize synthesized nanoparticles are SEM and UV–Visible spectrophotometer. UV–Visible spectrophotometer showed absorbance peak in range of 450–550nm. The silver nanoparticles showed antibacterial activities of Nymphaea odorataroot extract against both gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) microorganisms. The synthesized (silverNps) were found to be effective as antimicrobial agents against some important human pathogens
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    GREEN SYNTHESIS OF SILVER NANOPARTICLES USING MESUAFERREALINN FLOWER BUDS AND EVALUTION OF THEIR ANTI MICROBIAL ACTIVITIES
    (KSG college of Arts and Science, Coimbatore., 2018-08-11) Jeeva Dharshni S; Gomathi S; Sujithra V; Geetha M; Kanchana M
    Green synthesis of nano particles is an emerging branch of nano technology. There is an increasing commercial demand for nano particles due to their wide applicability in various areas, such as electronics, catalysis, chemistry, energy and medicine. Metallic nano particles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. In contrast the current study describes a cost effective and environment friendly technique for green synthesis of silver nano particles from 1mM. AgNo3 solution through the extract of species flower bud. Nano particles were characterized by using UV-Vis absorption spectroscopy, SEM analysis and elementary analysis, showed the average particle size of 46nm-101nm in chloroform extract and 37nm-66nm in ethanol extract as well as revealed their spherical and cubic structures respectively. Biologically synthesized nanoparticles were found to be highly antimicrobial against different multidrug resistant human pathogens
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    GREEN SYNTHESIS AND PHYTOCHEMICAL ANALYSIS OF SILVER NANO PARTICLES USING ROOT OF NELUMBO NUCIFERA AND THEIR ANTIBACTERIAL ACTIVITY
    (KSG College of Arts and Science, CBE., 2018-08-11) Geetha M; Muthumeenachi; Jeevadharshni S; Sujithra V; Kanchana M
    In the present study, biochemical analysis of Acalyphaindica,biosynthesis of silver nanoparticles and its antimicrobial activity on clinical laboratory bacterial pathogens were investigated. Silver nanoparticles were rapidly synthesized using root extract of Acalyphaindica and the formation of nanoparticles was observed. The results recorded from UV-vis spectrum, and scanning electron microscopy (SEM) support the biosynthesis and characterization of silver nanoparticles. Further, the antibacterial activity of synthesized silver nanoparticles showed effective inhibitory activity against clinical laboratory pathogens Viz., Escherichia coli (gram negative) and Staphylococcus aureus (gram positive).
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    BIODEGRADATION OF POLYTHENE BAG USING BACTERIA ISOLATED FROM SOIL
    (Kongunadu Arts and Science College, Coimbatore, 2018-08-07) Jeeva Dharshni S; Kanchana M
    Plastic are one of the most widely used materials and in most cases, they are designed to have long life time. Though plastics have become cosmopolitan, now days, they are very hazardous and should be disposed properly. Land filling, incineration and recycling are the most common methods employed for the disposal of plastics with the side effects of environmental and health hazards. So, biodegradation will be the right choice for the disposal of plastic wastes. Soil samples from the compost yard have the rich consortia of biodegrading microbes. These samples were inoculated into nutrient agar medium with plastic as the sole carbon source for the isolation of the plastic degrading strains. Characterization of bacterial strains was done based on morphological, biochemical and molecular characterization. The process of biodegradation was observed at regular interval for 30 days in a synthetic medium containing LDPE films. The bacterial isolates,Actinomycetes sp, Bacillus mycoidesis, Bacillus subtilis,Brevundimonasdiminuta, Pseudomonas putidaandPseudomonas stutzeriwere able to reduce the weight of the polymer up to 17%,14%, 30%, 22% 29% and 25% respectively. Degradation was monitored by observing weight loss and changes in physical structure by Fourier Transform Infrared (FTIR) Spectroscopy
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    MOLECULAR CHARACTERIZATION OF ENDOPHYTIC FUNGI (XYLERIASP)
    (Kongunadu Arts and Science College, CBE, 2018-08-07) Geetha M; Kanchana M
    Orchidaceae is one of the largest families with close to 25,000 species and encompassing 10% of flowering plants. Endophytic fungi are crucial, quantifiable and integral component of fungal biodiversity, and influenced by community diversity of plants and its structure. Fungal endophytes are also known for its role in ecological community by decreasing the range of environmental degradation, biodiversity loss, spoilage of water and land due to toxic insecticide, poisonous gases and industrial sewage. In this present study, the different Endophytic fungi like Fusarium sp.,Xylariasp., Westerdikellasp., Peniciilium sp. were isolated from two different orchid species Dendrobium sp. andBellaria sp. From four different isolates, xyleria sp. was selected for molecular identification. The genomic DNA was extracted from Xyleria sp. and which subjected to PCR using ITS 1 primer. The sequences were submitted in NCBI (National Centre for Biotechnological Information) for the analysis of homology.
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    IDENTIFICATION OF ACTIN GENE HOMOLOGUS FROM LEUCAS ASPERA
    (Hindustan College of Arts and Science, Coimbatore, 2015-02-25) Agnes Nirmala K; Kanchana M; Saranya M; UmaMaheswari P
    Leucas aspera commonly known as ‘Thumbai’ is used traditionally as an antipyretic and insecticide. It is distributed throughout India from the Himalayas down to Ceylon and possess various pharmacological activities like antifungal, antioxidant, antimicrobial, antinociceptive and cytotoxic activity. As this plant distributed throughout India, analysis of genes induced during abiotic stress may give better insights on abiotic stress tolerance. Quantitative real time RT-PCR is a better technique to analyse the differential expression of genes. A major step in gene quantification analysis is data normalisation in real-time RT-PCR . Normalizing through reference genes, or housekeeping genes, can make more accurate and reliable results from reverse transcription real-time quantitative polymerase chain reaction (qPCR). The classical housekeeping genes involved in basic cellular processes such as 18 S rRNA, ubiquitin, actin, b-tubulin, and glyceraldehyde-3-phosphate dehydrogenase have been recurrently used as internal controls for gene expression analysis in plant as they are supposed to have a uniform expression in all samples and experimental conditions tested. To analyse the differential expression of genes involved in abiotic stress, Actin gene homologue was identified from Leucas aspera to use as house keeping genein this experiment. Complete coding region (CDS) of actin gene from different plants were downloaded from NCBI database. Conserved region of the Actin gene was deduced and degenerate primers were designed using bioinformatics tools ClustalW and PriFi. Using the degenerate primers, partial gene homologue of actin from Leucas aspera genomic DNA was amplified and the amplicon was sequenced. The sequence data was annotated for exons and introns were submitted in NCBI database. This gene will be used as housekeeping gene for normalization in differential expression studies in this plant.