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Item RAW SEWAGE INDUCED BIOCHEMICAL CHANGES IN THE LIVER OF EDIBLE FISH, CHANNASTRIATA(Department of Biology, Gandhigram Rural University, Dindigul, Tamil Nadu, India, 2004-02-19) Sasikala G; Natarajan G M; Esther Joice P; Bhuvaneshwari N; Jacintha S; Palanisamy P; Binukumari NUntreated sewage (raw sewage) contains high levels of biochemical oxygen demand (BOD), suspended solids (SS), nitrogenous compounds, nutritive salts, bacteria and other pollutants. High BOD causes decrease in DO while DO lowers the lethal concentrations for various toxicants. Raw sewage is indiscriminately discharged into the various freshwater sources near metropolitan cities. Indiscriminate discharge of untreated sewage is a serious concern for freshwater fish culture in and around major cities. Very little information is available on the effects of raw sewage on the biochemical composition of freshwater food fishes, though a lot of studies have been conducted to assess the sublethal effects of environmental stresses on fish. Raw sewage (10%) exposure for 30 days inhibited the liver protein (-18.06%.; P<0.05) and lipid content (-51.65%.06; P<0.05) significantly in the edible fish, Channastriata. However, the cholesterol content (+51.21%.06; P<0.05) increased considerably. Changes in the pharmacological properties of the nervous system and damage to gills were also recorded for prolonged exposure. These results suggest that fish exposed to sewage are subject to severe biochemical perturbations and damage to vital organs.Item FISH MUCUS - AN IDEAL ADHESIVE FOR BUILDING CONSTRUCTIONMr(Department of Biology, Gandhigram Rural University, Dindigul, Tamil Nadu, India, 2004-02-19) Natarajan G M; Bhuvaneshwari N; Palanisamy P; Esther Joice P; Sasikala GFish skin contains a variety of humoral defense lysins which play an integral role in fish immunity and serve as the primary barrier between internal and external environment. This barrier consists of mucus and epidermal cell layers and contains a variety of active factors in the primary defense. Since fish are habitually surrounded by water, both the mucus and the active factors are no sooner secreted on the skin that dispensed into the surrounding water. The skin mucus lysins comprise bacteriolysins, proteolysins and haemolysins. The mucus is sticky and acts as a synthetic paste or adhesive. In the present investigation, an attempt has been made to explore the possibilities of using fish mucus as an adhesive in building construction activities. It is said that many of the old churches in Travancore – Cochin with arches and domes were built using Channastriata mucus. A normal method of preparing large quantities of mucus and mixing it with sand and bricks is outlined. A comparative study of the mucus secretion of the various air-breathing species like Clariasgariepnus, Anguilla bengalensis, Channastriata, C. maruilusandHeteropneustesfossilis reveals that Clariasgariepnus is an ideal candidate to obtain maximum quantity of mucus under laboratory conditions. Experiments with fish slime or mucus show that it is excellent in giving extra strength in the construction of big buildings.Item METASYSTOX INDUCED MOLECULAR IMMUNOLOGICAL MODULATIONS IN THE SOUTH-INDIAN EDIBLE FISH, CHANNASTRIATA(Department of Biotechnology, PSG College of Technology, Coimbatore Tamil Nadu, India, 2004-02-15) G, Sasikala; G M, Natarajan; N, Parthi; P, Esther Joice; S, Jacintha; G, TamilselviThe body cells of fish are endowed with various molecular defense mechanisms. Shortterm (15 days) and long -term (30 days) exposure of Sublethal concentration (1.7mg/l) of organophosphorous pesticide, metasystox triggered many defense reactions in the South Indian edible fish, Channastriata. The lysozyme activity, plasma immunoglobulin profile, splenic antibody secreting cells and lymphocytes increased significantly (P<0.05). Though the phagocytic activity increased, the number of yeast cells phagocytized decreased in the pesticide-exposed fishes. The respiratory burst activity and migration of kidney granulocytes showed irregular oscillations. RBC, Hb and Ht increased at all times. An initial rise in the blood glucose and lactate fell abruptly at long term exposure. Sodium and Potassium balance was suppressed. It is therefore abundantly clear that the pesticide stress induces several adaptive immunological modulation in Channastriata.