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    BRAIN ASCORBIC ACID CHANGES IN CLARIASGARIEPINUS DURING AIR-EXPOSURE AND SUBMERGENCE STRESSES
    (Department of Biology, Gandhigram Rural University, Dindigul, Tamil Nadu, India, 2004-02-19) Esther Joice P; Jacintha S; Bhuvaneshwari N; Palanisamy P; Sasikala G; Mallikaraj D; Natarajan G M
    Ascorbic acid is known to take part in collagen biosynthesis and in oxidation and reduction reactions of tissues. It is essential for various protective and defensive biological reactions of the organisms and perhaps acts as an antioxidant and fatigue retardant. Clariasgariepnus is an air-breathing fish. The fish is capable of living on land for extended periods. Similarly, its prolonged submergence in oxygenated water is also well established. However, its capacity to endure air-exposure and submergence stress has not been studied. This stress induced pharmacological and biochemical changes are worth studying and hence the present study. Nine hour air-exposure and submergence stress triggered differential biochemical response in the brain. Air-exposure stress decreased the ascorbic acid content of the cerebral hemispheres (-8.24%), optic lobes (-7.61%), cerebellum (-4.82%) and medulla oblongata (-6.88%) insignificantly. Submergence stress significantly decreased (P<0.05), the ascorbic acid content in all parts of the brain.
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    DESIGN OF AN AIR-EXPOSURE AND SUBMERGENCE STRESS NON-SPECIFIC IMMUNE RESPONSE MODEL IN THE EXOTIC FISH, OSPHRONEMUSOLFAX
    (Department of Biotechnology, PSG College of Technology, Coimbatore Tamil Nadu, India, 2004-02-15) Jacintha S; Natarajan G M; Esther Joice P; Parthi N; Sasikala G; Tamilselvi G
    The effects of air-exposure (6h) and submergence (6h) as monitored by determining RBC, Hb, Ht and blood glucose were analyzed in the exotic fish, Osphronemusolfax. Results obtained using multifactorial stress model (air-exposure, submergence, hypoxia) showed that elevation of RBC, Hb, Ht and blood glucose was linearly correlated with progressive stress uniformly. Submergence caused rapid stress with blood glucose reaching 50% elevation within 60 min. Hypoxic water with access to air had very little effect on blood glucose. Similarly, lowering the temperature significantly affected the blood parameters. The airexposed model was also used for testing lysozyme activity and phagocytosis assay during stress. Contrary top blood glucose, plasma lysozyme was significantly lower in air stressed than in unstressed fish. Submergence increased the numbers of granulocyte, lymphocyte.