Browsing by Author "Tahani, Awad Alahmadi"

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ANTIMICROBIAL, ANTIOXIDANT, ANTICANCER, AND ANTITHROMBOTIC, COMPETENCY OF SAPONINS FROM THE ROOT OF DECALEPIS HAMILTONII
(Elsevier, 2023-08-15) Jayaraman, Gitanjali; Duraisamy, Sumathi Dinesh Ram; Kavitha R; Venkatesan, Amalan; Tahani, Awad Alahmadi; Sulaiman Ali, Alharbi; Sabariswaran, Kandasamy; Rajasree, Shanmuganthan; Natesan, Vijayakumar
The goal of this study was to extract saponins from the tuberous root of Decalepis hamiltonii and assess their potential clinical applications, which included antioxidant, antibacterial, antithrombotic, and anticancer properties. Surprisingly, the results of this study revealed that the extracted saponins have excellent antioxidant activities, as demonstrated by 2,2-diphenylpicrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Hydrogen peroxide (H2O2), and Nitric oxide (NO) scavenging assays. Nonetheless, at a concentration of 100 g/mL, crude saponin had excellent antibacterial activity, particularly against gramme positive bacteria (Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis, and Micrococcus luteus), followed by gramme negative bacteria (Escherichia coli, Salmonella typhi, Proteus mirabilis, and Klebsiella pneumonia). Despite this, the crude saponin had no effect on Aspergillus niger and Candida albicans. The crude saponin also possesses outstanding in vitro antithrombotic activity on blood clot. Interestingly, the crude saponins have an outstanding anticancer activity of 89.26%, with an IC50 value of 58.41 μg/mL. Overall, the findings conclude that crude saponin derived from D. hamiltonii tuberous root could be used in pharmaceutical formulations.
ANTIOXIDANT AND ANTICANCER POTENTIAL OF ETHYL ACETATE EXTRACT OF BARK AND FLOWER OF TECOMA STANS (LINN) AND IN SILICO STUDIES ON PHYTOLIGANDS AGAINST BCL 2 AND VEGFR2 FACTORS
(Elsevier, 2023-02-15) Mathiyazhagan, Narayanan; Anburaj, Gothandapani; Rajasudha, Venugopalan; Manikandan, Rethinam; Sakunthala, Pitchai; Tahani, Awad Alahmadi; Hesham S, Almoallim; Sabariswaran, Kandasamy; Kathirvel, Brindhadevi
This study was designed to appraise the antioxidant and anticancer competence of solvent extracts of Tecoma stans (Linn) and analyze the phytoligands interaction against Bcl 2 VEGFR2 through in silico studies. The phytochemical analysis revealed that the ethyl acetate extract contains more number of pharmaceutically valuable phytochemicals than other solvent extracts. Among the various phytochemicals, flavonoid was found as a predominant component, and UV–Vis- spectrophotometer analysis initially confirmed it. Hence, the column chromatogram was performed to purify the flavonoid, and High-performance liquid chromatography (HPLC) was performed. It revealed that the flavonoid enriched fraction by compared with standard flavonoid molecules. About 84.69% and 80.43% of antioxidant activity were found from ethyl acetate extract of bark and flower at the dosage of 80 μg mL−1 with the IC50 value of 47.24 and 43.40 μg mL−1, respectively. In a dose-dependent mode, the ethyl acetate extract of bark and flower showed cytotoxicity against breast cancer cell line MCF 7 (Michigan Cancer Foundation-7) as up to 81.38% and 80.94% of cytotoxicity respectively. Furthermore, the IC50 was found as 208.507 μg mL−1 and 207.38 μg mL−1 for bark and flower extract correspondingly. About 10 medicinal valued flavonoid components were identified from bark (6) and flower (4) ethyl acetate extract through LC-MS analysis. Out of 10 components, the 3,5-O-dicaffeoylquinic acid (ΔG -8.8) and Isorhamnetin-3-O-rutinoside (ΔG -8.3) had the competence to interact with Bcl 2 (B-Cell Lymphoma 2) and VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) respectively with more energy. Hence, these results confirm that the ethyl acetate extract of bark and flower of T. stans has significant medicinal potential and could be used as antioxidant and anticancer agent after some animal performance study.
EFFECTS OF BIOHYDROGEN ON PREMIXED CHARGE COMPRESSION IGNITION ENGINE OPERATING AT VARIOUS INLET AIR TEMPERATURES
(Elsevier, 2023-01-15) Bingchao, Zhao; Xiaoyong, Song; Han, Wang; Sabariswaran, Kandasamy; Arunachalam, Chinnathambi; Tahani, Awad Alahmadi; Govindasamy, Sathiyan; Nguyen, Thuy Lan Chi; Rajasree, Shanmuganathan
The usage of the diesel engine has been increasing every year and simultaneously the emissions by the fossil fuels are mounting up and damaging the environment. Hence, it is of profound interest to seek the solution to reduce the emissions released by fossil fuels. In this proposed study, the biohydrogen influence on the diesel engine has been examined. Since biohydrogen is a green gas, it reduces the emissions of certain pollutants significantly. Hence, here the diesel engine is tested with 10% and 30% of biohydrogen flow through air intake. Two different combinations such as DH10 (10% Biohydrogen and 90% diesel) and DH30 (30% biohydrogen and 70% diesel) were tested at different mean effective pressure (MEP). The MEP has been varied from 1.05 bars to 4.2 bars. Since the engine is operating on premixed charge compression ignition conditions, inclusion of the biohydrogen is challenging. To avoid biohydrogen backfire flame arrestor has been attached between the cylinder and flowmeter. The crucial parameters such as brake thermal efficiency, brake specific fuel consumption, heat release rate, cylinder pressure, combustion timing, and emissions data were collected and analyzed to understand the sustainability of the biohydrogen gas running on the diesel engine. Based on findings it is clear, adding the biohydrogen gas to the engine increases the BTE and HRR. Further the cylinder pressure values were also increased due to the usage of compressed gas. Nevertheless, the brake specific fuel consumption has been at higher levels due to the energy discrepancy. With regards to the emission, CO, CO2 and NOx concentration in the exhaust tailpipe has decreased marginally, which is pleasing. Comparing DH10 and DH30, DH30 outperforms with regard to emission data. On the other hand, the performance values are superior for DH10 than DH30.