Browsing by Author "Sharmila Chandran"

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    BIOSYNTHESIS OF PVA ENCAPSULATED SILVER NANOPARTICLES
    (UNAM, Centro de CienciasAplicadas y DesarrolloTecnológico, 2016-10-01) Sharmila Chandran; Vinuppriya Ravichandran; Selvi Chandran; Jincy Chemmanda; Bellan Chandarshekar
    Green synthesis of metal nanoparticles is an important technique in the methods of eco-friendly nanoparticle production. The synthesis of silver nanoparticles was accomplished using Ocimum sanctum leaf extract at room temperature. These particles were then encapsulated with polyvinyl alcohol (PVA) polymer matrix. The presence of silver was confirmed by different characterization techniques such as UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-Ray Diffraction (XRD). Scanning electron microscopic (SEM) images of the synthesized powder shows spherical shaped silver nanoparticles embedded in sponge-like polymer matrix. The energy dispersive X-ray analysis confirms the presence of elemental silver along with iron signal. Energy dispersive signal corresponding to elemental iron has been attributed to O. sanctum plant. The silver nanoparticles in PVA matrix thus obtained shows high antibacterial activity against gram positive Staphylococcus aureus (S. aureus) and gram negative Escherichia coli (E. coli) water borne bacteria. The inhibition zone against S. aureus and E. coli were also calculated.
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    DEXTRAN SULFATE STABILIZED SILVER NANOPARTICLE: NEXT GENERATION EFFICIENT THERAPY FOR CANCER
    (Innovare Academic Sciences Pvt Ltd, 2020-01-01) Sharmila Chandran; Thilagavathi Ponnusamy; Dinesh Bheeman; Ranjith Rajamani; Chandarshekar Bellan
    Objective: Synthesize silver nanoparticles using a green synthesis approach and encapsulate silver nanoparticles with a naturally occurring polymer, particularly of an-hydroglucose type, like dextran sulfate sodium salt and to study its anticancer activity. Methods: Green synthesis approach is been employed in the synthesis of silver nanoparticles using Psidiumguajava leaf extract. The nanoparticles were then encapsulated with dextran sulfate biopolymer and the nanoparticles were subjected to different characterization techniques. The structure of the synthesized nanoparticles was analyzed using X-ray diffraction analysis, the presence of different functional groups was analyzed by FTIR studies. Size and morphology of the prepared nanoparticles were investigated using FESEM analysis. Anticancer activity of the synthesized nanoparticles was tested against the MCF-cell line. Results: The XRD analysis shows the crystalline nature of the synthesized nanoparticles. The stretching and vibrating modes of different functional groups were confirmed by FTIR result. The SEM image confirmed the presence of spherical shaped nanoparticles and the TEM image confirmed the average size of the particles to be around 24 nm. The Ag-DS NPs showed 91% cell inhibition for the concentration of 100 μg/ml, indicating the cytotoxicity of the nanoparticles against MCF-7 cell line. Conclusion: Dextran sulfate stabilized silver nanoparticles show potent anticancer activity against MCF-7 cell line.
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    ENHANCED ANTIMICROBIAL ACTIVITY OF ALOE VERA BLENDED ZINC OXIDE NANOPARTICLES IN PVA MATRIX
    (Elsevier, 2018-01-01) Sharmila Chandran; JincyChemmanda Sunny; Selvi Chandran; Chandrashekar Bellan
    Aloe vera, a traditional medicinal plant belongs to Liliaceae family that has been adored for centuries as a remedy for skin infections & cuts. The active ingredients such as berberine and gallic acid present in Aloe vera plays a major role in making the plant a therapeutic agent. In order to increase the efficiency of the plant, ZnO is blended with Aloe vera gel. The Aloe vera blended ZnO nanoparticles was then encapsulated using Polyvinyl Alcohol (PVA) polymer and were subjected to different characterization techniques. The cell viability of Aloe vera blended ZnO nanoparticles in PVA matrix was evaluated and in vitro studies against wound creating pathogens were carried out.
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    HYBRID PVA-IN2O3 NANO THIN FILM FOR TRANSPARENT OPTICAL DEVICES
    (IJAERD, 2017-12-01) Sathish Sugumaran; Dinesh Muthu; Chandar ShekarBellan; Dinesh Bheeman; Sharmila Chandran
    Hybrid PVA-In2O3transparentnano thin film (293 nm) was prepared by very simple and cost effective dip coating method. The effect of annealing temperature on the functional group, structure, morphology and optical properties was investigated. The presence of metal-oxide (In-O) bond was confirmed from Fourier transform infrared spectroscopy. X-ray diffraction patterns revealed the predominantly amorphous in nature of the films. Scanning electron microscopy images revealed spherical shaped uniform grains distributed over the entire film surface. The sizes of the grains increased with increase of annealing temperature. The percentage of transmittance (80 to 90%) increases whereas band gap energy (3.80 to 3.76 eV) values decreases with increase of annealing temperature. The obtained results indicated that the amorphous nature and high transmittance with wide band gap of the prepared transparent hybrid nano thin films indicated the feasibility of utilizing these nano thin films in transparent optical device applications.
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    SHAPE CONTROLLED SYNTHESIS OF DEXTRAN SULFATE STABILIZED SILVER NANOPARTICLES: BIOCOMPATIBILITY AND ANTICANCER ACTIVITY
    (IOP Publishing, 2019-01-16) Sharmila Chandran; Prabhavathi Venkatesan; Dinesh Bheeman; Ranjith Rajamani; Chandarshekar Bellan
    Three Dimensional button, flower and sphere shaped microstructure of silver nanoparticles dispersed in dextran sulfate matrix were synthesized using silver nitrate, trisodium citrate and dextran sulfate. Three different amount of dextran sulfate (2 drops, 10 drops and 15 drops) were added to each 10 ml of silver nanoparticles to make three different solution mixtures, which was then subjected to different characterization techniques. The XRD study of flower shaped dextran sulfate stabilized silver nanoparticles showed a diffraction pattern corresponding to face centered cubic structure of Ag crystals. The FESEM image shows a well defined three dimensional button shaped microstructure for 2 drops of dextran sulfate, flower shaped microstructure for 10 drops of dextran sulfate and sphere shaped microstructure for 15 drops of dextran sulfate. Based on the morphological structure of the synthesized nanoparticle the absorption property was discussed, the absorption band varied from 429 nm to 434 nm. The nanoparticles prepared using dextran sulfate of high concentration (15 drops) shows a blue shift in absorbance spectra, indicating smaller size of AgNPs with high absorbance property. The results reveal that the surface morphology affects the absorption behavior of the nanoparticles. The results of cytotoxicity assay against human vero cell lines revealed that flower shaped dextran stabilized AgNPs shows ≥ 90% cell viability indicating the biocompatibility of the nanoparticles. The In-vitro anticancer activity of the synthesized Ag-DS nanoparticles against human breast cancer cell line MCF-7, was studied. The results of the present study indicated that the Ag-DS nanoparticles can be a potent anticancer agent.