Browsing by Author "Saleh, Alfarraj"

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    AN ASSESSMENT OF METAL ABSORPTION COMPETENCE OF INDIGENOUS METAL TOLERANT BACTERIAL SPECIES- AN IN-VITRO STUDY
    (Elsevier, 2024) Anusha, P; Natarajan, D; Sumathy, Rengarajan; Saleh, Alfarraj; Sabariswaran, Kandasamy
    Heavy metals pose a serious global threat to the environment. Hence, removing hazardous metals from soil samples has become complicated over the past few years. The current work looked into the remediation of heavy metals from aqueous solutions using a bacterial community and a unique bacterium obtained from metal-contaminated soil. In this investigation, the isolates of Bacillus anthracis A1-7, Bacillus. thuringiensis A1-3, Bacillus. cereus A1-5, and Pseudomonas aeruginosa A-33 actively demonstrated metal tolerances to various tested metals. Furthermore, an in-vitro biosorption study was performed under ideal concentration. The bacterial consortia achieved the highest biosorption effectiveness for Cu & Zn, 92.7% and 90.3%, respectively. When compared with a single bacterium, the group exhibited inferior Pb biosorption (86%). Since then, P. aeruginosa A33 has had the highest Pb biosorption. Finally, a bacterial consortium has devised an intriguing strategy for eliminating Cu and Pb from the polluted medium. P. aeruginosa A33 was found to be a mighty microbe that extracts Zn from polluted water. This metal-tolerant bacterium also exhibited specific proportions of selective commercially available antibiotics, which were analyzed using the Multiple Antibiotic Resistance (MAR) Index. In conclusion, these findings indicated that bacterial consortia composed of four bacterial isolates can remove metals from a metal-polluted medium.
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    AN ASSESSMENT OF METAL ABSORPTION COMPETENCE OF INDIGENOUS METAL TOLERANT BACTERIAL SPECIES- AN IN-VITRO STUDY
    (Elsevier Inc, 2024-06) Anusha, P; Natarajan, D; Sumathy, Rengarajan; Saleh, Alfarraj; Sabariswaran, Kandasamy
    Heavy metals pose a serious global threat to the environment. Hence, removing hazardous metals from soil samples has become complicated over the past few years. The current work looked into the remediation of heavy metals from aqueous solutions using a bacterial community and a unique bacterium obtained from metal-contaminated soil. In this investigation, the isolates of Bacillus anthracis A1-7, Bacillus. thuringiensis A1-3, Bacillus. cereus A1-5, and Pseudomonas aeruginosa A-33 actively demonstrated metal tolerances to various tested metals. Furthermore, an in-vitro biosorption study was performed under ideal concentration. The bacterial consortia achieved the highest biosorption effectiveness for Cu & Zn, 92.7% and 90.3%, respectively. When compared with a single bacterium, the group exhibited inferior Pb biosorption (86%). Since then, P. aeruginosa A33 has had the highest Pb biosorption. Finally, a bacterial consortium has devised an intriguing strategy for eliminating Cu and Pb from the polluted medium. P. aeruginosa A33 was found to be a mighty microbe that extracts Zn from polluted water. This metal-tolerant bacterium also exhibited specific proportions of selective commercially available antibiotics, which were analyzed using the Multiple Antibiotic Resistance (MAR) Index. In conclusion, these findings indicated that bacterial consortia composed of four bacterial isolates can remove metals from a metal-polluted medium.
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    SCREENING, CHARACTERIZATION, AND OPTIMIZATION OF LIPASE ENZYME PRODUCING BACTERIA ISOLATED FROM DAIRY EFFLUENTS CONTAMINATED MUDDY SOIL
    (SpringerLink, 2023) Sabariswaran, Kandasamy; V S, Vijayalakshmi; Saleh H, Salmen; Saleh, Alfarraj; Milton, Wainwright; Devarajan, Natarajan
    Lipases, particularly microbial lipases, are important industrial biocatalysts. As a result, lipase enzyme screening, synthesis, and purification from microbial strains are constantly evolving to meet the needs of the pharmaceutical and food industries. Thus, the goal of this study was to identify the most potential lipase-producing bacterial strains from Aavin dairy industry effluent contaminated soil. Furthermore, growth parameters, such as pH, temperature, carbon and nitrogen sources, were optimized for lipase enzyme production from selected bacterial strains. According to the findings, 9 strains (V1–V9) of 15 bacterial isolates were found to be lipase producers. However, three strains (V1, V7, and V8) predominated and demonstrated significant lipase-producing activity. These V1, V7, and V8 bacterial strains were identified as Bacillus pumilus V1, Bacillus pumilus V7, and Bacillus subtilis V8 through 16S rRNA sequencing. About 16.6 to 27.8 µg mL−1 of lipase production was recorded under the optimal growth conditions: pH 8, temperature 37 °C, fructose and yeast extract as suitable carbon and nitrogen source. Among these 3 strains B. pumilus V1 showed excellent lipase productivity than others. The molecular weight of this lipase produced by bacteria was determined to be 35 kDa using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).