Browsing by Author "Rosaline Mary"

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    BIOSYNTHESIS OF SILVER NANOPARTICLES FROM THE NESTS OF THE PAPER WASP, ROPALIDIA MARGINATA AND THE MUD WASP, SCELIPHRON CAEMENTARIUM AND THEIR ANTIMICROBIAL ACTIVITIES
    (UBI Journals, 2018) Susheela P; Rosaline Mary; Radha R
    Nanotechnology forms one of the emerging subjects of research with regard to the modern biological and material sciences. In the present study we have reported, bio synthesis of silver nanoparticles (AgNps) from the nests of the paper wasp, Ropalidiamarginata (PE) and mud wasp, Sceliphroncaementarium (ME) and confirmed their antimicrobial activities. The AgNps were characterized by UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy dispersive electron spectroscopy (EDAX) and X-ray diffraction (XRD). The AgNps were evaluated for antimicrobial properties both antibacterial and antifungal. The board peaks were obtained at 389 nm for PE and 400 nm for ME with UV-Vis surface plasmon resonance studies confirmed that the synthesized nanoparticles are AgNps. The FTIR data showed prominent peaks for both which indicated the presence of phenolics compounds and proteins in the synthesis of AgNps. Scanning electron microscopic (SEM) studies revealed that the nanoparticles were spherical in shape with size ranging from 0.1 to 0.5 µm for PE and 0.2 to 0.5 µm for ME. EDAX analysis showed 75.48 weight percentage of Ag present in PE and 72.57 % in ME indicated the purity of sample. The AgNps of PE showed potent antibacterial activity against S. pyogenes, S. aureus, E.coli, K. pneumoniae, B. subtilis, S.paratyphi and antifungal activity against C. albicans, T. viride and A. fumigatus. The PE AgNps produced zone of inhibition of 13 mm against K. pneumoniaeand 8 mm against T.viride at 30µg/ml. The AgNps of ME showed potent antibacterial activity against S. pyogenes, S.aureus, E.coli, K.pneumoniae, Bacillus subtilis, S. paratyphi and antifungal activity against Candida albicans, T. viride, Afumigatus. The PE AgNps produced zone of inhibition of 12 mm against S. aureus and 9 mm against T. viride at 30 µg/ml. Pencillin standard drug (10µg) was used as positive control for both bacteria and fungus
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    GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS IN THE ETHANOLIC EXTRACT OF NEST MATERIAL OF MUD DAUBER WASP, SCELIPHRON CAEMENTARIUM
    (Innovare Academic Sciences Pvt Ltd, 2016) Susheela P; Rosaline Mary; Radha R
    The objective of the present study was to determine the chemical compounds present in the nests of the mud dauber wasp, Sceliphron caementarium.Methods: Gas chromatography-mass spectrometry analysis of the nest samples was carried out by standard procedures. The resultant compounds were compared with the database of the National Institute Standard and Technology (NIST), WILEY8, FAME.Results: The results of the gas chromatography-mass spectrometry analysis of the concentrated ethanol extract revealed the presence of chemical compounds such as methylene chloride, 1, 1’:3’, 1’’-Terphenyl, 5’-Phenyl, Di N Decylsulfone, Eicosanoic acid, 1, 2-Bis (Trimethylsilyl) Benzene, and Androstane-11, 17-Dione, 3-[(Trimethylsilyl) Oxy]-, 17-[O-(Phenylmethyl) O.Conclusion: The compounds identified were found to have biological properties such as anti-inflammatory, antibacterial, and antifungal, and further study of these isolated compounds may prove their medicinal importance in future
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    IN-VITRO STUDY OF THE ANTICANCER ACTIVITIES OF SILVER NANOPARTICLES SYNTHESIZED FROM THE NESTS OF ROPALIDIA MARGINATA AND SCELIPHRON CAEMENTARIUM
    (UBI Journals, 2018) Susheela P; Rosaline Mary; Radha R
    Nests of the paper wasp, Ropalidia marginata and mud nest wasp, Sceliphron caementarium were used for the biological synthesis of silver nanoparticles (AgNPs). The objective of the present work was towards the evaluation of in-vitro cytotoxic activity of silver nanoparticles synthesized from the nest extracts of the paper wasp (PE), and mud wasp, (ME) against breast cancer cell lines (MCF 7) and cervical cancer cell lines (HeLa). Characterization of the AgNPs were done by UV-Visible spectrometry, Scanning electron microscopy (SEM), Energy dispersive X-ray analysis (EDAX), X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR). The UV spectrophotometeric analysis of AgNPs displayed maximum absorption at 389 nm for PE and 400 nm for ME. Prominent peaks were obtained with FTIR data indicating the presence of phenolics compounds and proteins. SEM studies revealed the spherical nature of the nanoparticles with the size ranging from 0.1 to 0.5 μm (PE) and 0.2 to 0.5 μm (ME). The weight percentage of Ag present in PE was 75.48 % and 72.57 % for ME indicating the purity of sample during EDAX analysis. MTT assay results revealed that AgNPs exhibited potent cytotoxicity against MCF 7 cell lines with IC50 value for18.98μg/ml with regard to paper wasp extract (PE) and 22.70μg/ml for mud nest wasp (ME).And for the HeLa cell lines the values were 23.10μg/ml for PE and 29.02μg/ml for ME. Thus the present study concludes that the bio synthesized AgNps exhibit anticancer properties and these nest extracts can be used to develop into an anticancer formulation of clinical interest