Browsing by Author "Ramesh M"

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ACUTE AND SUBLETHAL INTOXICATION OF DELTAMETHRIN IN AN INDIAN MAJOR CARP, LABEO ROHITA: HORMONAL AND ENZYMOLOGICAL RESPONSES
(The Journal of Basic & Applied Zoology, 2015-04-03) Suvetha L; Saravanan M; Hur JH; Ramesh M; Krishnapriya K
This study aimed to evaluate the median lethal concentration (LC50), acute (24 and 96 h) and sublethal (35 d) effects of deltamethrin, a synthetic pyrethroid pesticide on hormonal and enzymological responses in an Indian major carp, Labeo rohita. In this study, the LC50 values of deltamethrin for 24 and 96 h were found to be 0.438 and 0.38 mg L-1, respectively. During acute (0.438 mg L-1) and sublethal (1/10th of 24 h LC50 value, 0.0438 mg L-1) studies, plasma cortisol and prolactin levels were significantly increased (p< 0.05). When compared with the control group, a significant (p< 0.05) increase in alkaline phosphatase (ALP) activity was observed in liver and kidney of fish treated with deltamethrin. However, a significant decrease in the activity of acid phosphatise (ACP) was observed in liver and kidney of deltamethrin exposed fish. In addition, cholinesterase (ChE) activity was significantly (p <0.05) decreased in the plasma of fish exposed to both acute and sublethal concentrations of deltamethrin. These results suggest that the tested concentrations of deltamethrin could have significant adverse effects on the hormonal and enzymological parameters of fish L. rohita. The alterations of these parameters can be effectively used to monitor the impact of deltamethrin in aquatic ecosystem.
COMPARATIVE TOXICITY OF COPPER (II) OXIDE NANOPARTICLES WITH ITS BULK COUNTERPART IN AN INDIAN MAJOR CARP LABEO ROHITA
(TamilNadu Agricultural University, 2016) Krishnapriya K; Ramesh M
Nanotoxicology was a new branch of science which dealt with the fate of the nanoparticles in the environment and its harmful effects on nontarget organisms. The metallic copper (CuSO4) is a well known toxicant to aquatic organisms especially to fishes. As the size of the metal decrease to nano level, its surface to volume ratio would increase. This resulted in drastic changes in its physical, biochemical, catalytic and toxicity properties. In the view of the above context the present study was aimed to compare the toxicity of copper and nano form of copper in a freshwater fish Labeo rohita using certain biomarkers.
ECOLOGICAL RISK ASSESSMENT OF SILICON DIOXIDE NANOPARTICLES IN A FRESH-WATER FISH LABEO ROHITA: HEMATOLOGY, IONOREGULATION AND GILL NA+/K+ATPASE ACTIVITY
(Ecotoxicology and Environmental Safety, 2015-05-19) Krishnapriya K; Ramesh M; Saravanan M; Ponpandian N
The fate and effect of nanomaterials in the environment has raised concern about their environmental risk to aquatic organisms. Silica nanoparticles (SiO2-NPs) find its uses in various fields and are inevitably released into the environment. However, the ecotoxicological effects of SiO2-NPs on the freshwater fish remain poorly understood. The aim of this study was to evaluate the effect of different concentrations (1, 5 and25mgL_1) of SiO2-NPs on certain hematological, ionoregulatory and enzymological profiles of a freshwater teleost fish Labeo rohita. Hematological parameters such as hemoglobin(Hb), hematocrit (Hct), red blood cells (RBC), white blood cells (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) values were altered in SiO2-NPs treated groups. Likewise, plasma electrolytes such as plasma sodium (Na+), potassium (K+) and chloride (Cl-) levels and Na+/K+ATPase activity in gill of SiO2-NPs treated groups were altered in all concentrations throughout the study period (96h). The alterations of these parameters were found to be dependent on dose and exposure period. The results of the present study indicate that the alterations of these parameters may relate to physiological stress system to SiO2-NPs toxicity and also demonstrate that manufactured metaloxide NPs in aquatic environment may affect the health condition of the aquatic organisms
SUBLETHAL CONCENTRATION OF BISPHENOL A INDUCES HEMATOLOGICAL AND BIOCHEMICAL RESPONSES IN AN INDIAN MAJOR CARP LABEO ROHITA
(Ecohydrology & Hydrobiology, 2017-07-04) Krishnapriya K; Shobana G; Narmadha S; Ramesh M; Maruthappan V
Bisphenol A (BPA), an organic compound which is widely used in the production of synthetic polymers, has been detected in surface water, sediments and biota and emerged as a ubiquitous contaminant in the aquatic environment. In the present study, Labeo rohita was exposed to sublethal concentration of BPA and hematological and biochemical responses were studied. The median lethal concentration of BPA to L. rohita was evaluated by probit analysis method and the value was calculated as 7.3 mg/L for 24 h. Fish were exposed to a sublethal concentration of 0.73 mg/L (1/10th of the 24 h LC50 value) of BPA for 35 days and hemato-biochemical and marker enzyme assays was performed at the end of 7, 14, 21, 28 and 35 days. The results revealed that there was a significant (P < 0.05) decrease in hematological (hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin) and biochemical (protein) parameters in the BPA treated fish compared to control groups. White blood cells, glucose, aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase parameters were enhanced in experimental fish relative to control groups. However, the response of red blood cells, mean corpuscular hemoglobin concentration and gill Na+/K+-ATPase activity were found to be biphasic. In conclusion, the present investigation showed that analysis of hematological and biochemical parameters can be used as biomarkers for monitoring of BPA in the aquatic ecosystem.