Browsing by Author "Ramalingam, Sathishkumar"

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    CARROT ANTIFREEZE PROTEIN ENHANCES CHILLING TOLERANCE IN TRANSGENIC TOMATO
    (Springer Link, 2014) Sarma, Rajeev Kumar; Rajamani, Kiruba; Srinivasan, Balamurugan; Hélia G, Cardoso; Arnholdt-Schmitt, Birgit; Ahmed, Zakwan; Ramalingam, Sathishkumar
    In an attempt to improve chilling tolerance, the carrot gene encoding the antifreeze protein (AFP) was cloned under the control of constitutive CaMV35S promoter and genetically transformed the tomato var. PKM1 using Agrobacterium-mediated genetic transformation. Putative transgenic plants were confirmed by PCR using AFP-specific primers and grown to maturity. The integration of AFP transgene in the tomato genome was confirmed by Southern blot analysis. The AFP gene expression in transgenic plants was determined using semi-quantitative reverse transcription PCR. Upon exposure to chilling stress (4 °C), a significant decrease in membrane injury index was observed in AFP transgenic tomato lines without any phenotypic aberrations when compared with WT plants. Hence, this study clearly proves that the development of chilling tolerant tomato plants will soon become a reality.
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    CARROT ANTIFREEZE PROTEIN ENHANCES CHILLING TOLERANCE IN TRANSGENIC TOMATO
    (Springer Berlin Heidelberg, 2014) Sarma, Rajeev Kumar; Rajamani, Kiruba; Srinivasan, Balamurugan; Helia G, Cardoso; Arnholdt, Schmitt Birgit; Ahmed, Zakwan; Ramalingam, Sathishkumar
    In an attempt to improve chilling tolerance, the carrot gene encoding the antifreeze protein (AFP) was cloned under the control of constitutive CaMV35S promoter and genetically transformed the tomato var. PKM1 using Agrobacterium-mediated genetic transformation. Putative transgenic plants were confirmed by PCR using AFP-specific primers and grown to maturity. The integration of AFP transgene in the tomato genome was confirmed by Southern blot analysis. The AFP gene expression in transgenic plants was determined using semi-quantitative reverse transcription PCR. Upon exposure to chilling stress (4 C), a significant decrease in membrane injury index was observed in AFP transgenic tomato lines without any phenotypic aberrations when compared with WT plants. Hence, this study clearly proves that the development of chilling tolerant tomato plants will soon become a reality.
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    UNRAVELLING THE PHARMACOLOGICAL APPLICATIONS AND TAXONOMICAL CHALLENGES IN CANSCORA SPECIES USING DNA BARCODES (TAXON-SPECIFIC DNA AND HIGH-THROUGHPUT DNA BARCODES): A SYSTEMATIC REVIEW (Review)
    (Springer, 2025-03-25) Rajamani, Kiruba; Al Huq, Mohammed Suhaib; Selvaraj, Dhivya; Ramalingam, Sathishkumar
    The utilization of herbal-based remedies for disease prevention and treatment has proved its resilience, making a substantial contribution to the progress of traditional medicine. This therapeutic approach has demonstrated the efficacy of herbal drugs and has been an enduring practice. As a routine practice, the identification of plant species relies on morphological characteristics. However, there exist scenarios where the conventional strategies are inadequate due to the insufficient amount of material available for the examination. Molecular approaches for species distinction are promising, recognizing DNA or metabolite differences among taxa as biomarkers. This review focuses on the genus Canscora, known for its wide range of pharmacological uses, including antibacterial properties, anti-inflammatory, anticancer, and antioxidant. We explore the taxonomic challenges of this genus, which is made up of synonyms and morphologically overlapping features. We highlight the utility of DNA barcodes for accurate species identification and focus on investigating the taxon-specific, high-throughput DNA barcoding methods that emphasize the efficiency in validating molecules. Our findings highlight the potential of DNA barcoding to overcome the limitations of traditional techniques. This guarantees the accurate identification of Canscora species for medical purposes. The creation of a comprehensive DNA barcode library is recommended to promote phytochemical research, increase drug awareness, and protect biological diversity.