Browsing by Author "Padma Devi, S N"

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ASSESSMENT OF COMPETENCE OF THE PSEUDOMONAS AERUGINOSA TO SOLUBILIZE INSOLUBLE FORM OF ZINC UNDER VARIOUS CULTURAL PARAMETERS
(Springer Link, 2016) Padma Devi, S N; Sunitha kumari, K; Vasandha, S
Efficiency of Pseudomonas aeruginosa to solubilize insoluble form of zinc to soluble form was studied under various cultural parameters such as carbon (glucose, fructose, sucrose, maltose and lactose), nitrogen (ammonium sulphate, sodium nitrate, potassium nitrate and urea), pH (5.0, 7.0 and 9.0) and temperature (20, 30 and 40°C). The experimental study was carried out by both qualitative (plate assay) and quantitative (broth assay) methods. The Zn-solubilizing efficiency of the isolate was found more when glucose was added as C-source in both plate (150 %) and broth (16.62 mg/l) assays, and the efficiency was less in the medium amended with sucrose as carbon source in both qualitative (105.8 %) and quantitative (2.56 mg/l) estimation. Among the four nitrogen sources, P. aeruginosa showed best solubilizing efficiency (262.5 %) and solubilization of Zn (29.91 mg/l) in the presence of ammonium sulphate and recorded least solubilization efficiency in the presence of sodium nitrate as nitrogen source in both plate (181.82 %) and broth (21.92 mg/l) assays. It showed highest solubilization of zinc from ZnO at the incubation temperature of 30°C, and the activity was less at the temperature of 40°C. pH 7 was the most favourable pH for solubilization, and the efficacy was least when the pH reached its alkalinity. Thus the study confirmed the efficiency of P. aeruginosa to solubilize insoluble form of zinc and can be used as a source of bioinoculants to eradicate zinc deficiency in plants.
BIODEGRADATION OF LIGNOCELLULOSIC COIR PITH BY USING FUNGAL FORMS (Article)
(EM International, 2006) Vinodhini, S; Padma Devi, S N; Padma, Srinivasan
The present study deals with degradation of coir poll by Humicola sp. Trichoderma viride and Aspergillus terreus. The lignin, cellulose, organic carbon content and evolution of carbon-di-oxide were estimated in pith degraded aad non-degraded coir pith. The content of lignin and cellulose were found to be low in lignin and cellulose during different period of biodegradation.
EFFICIENCY OF ASPERGILLUS TERREUS TO SOLUBILIZE INSOLUBLE FORM OF PHOSPHORUS INTO SOLUBLE FORM UNDER VARIOUS CULTURAL CONDITIONS
(International Journal of Pharma and Bio Sciences, 2016-03) Anitha, S; Padma Devi, S N
Phosphorous forms the second major nutrient required by the plants for its growth. It is supplied to the plants in the form of synthetic fertilizer which gets accumulated in the soil due its conversion into unavailable form. Transformation of unavailable form of phosphorus (P) to an available form becomes inevitable due to its physiological and biochemical role in plants. Since its deficiency results in the cessation of plant growth, supplementation of soluble form of phosphorus becomes essential. Fungus has the capability to convert insoluble form of phosphorus to soluble form through the secretion of organic acid as one of the solubilization mechanism. Thus in the present study, Aspergillus terreus was isolated from the rhizospheric soil sample of tomato field and was tested for its phosphorus solubilization efficacy by Broth assay method. The strain showed highest solubilization (7.5 mg/l) at the 10th day of incubation with the pH drop of 4.0. Solubilization efficacy of the isolate was high due to the secretion of organic acid (gluconic acid) which was confirmed by HPLC method. Effect of various parameters such as Carbon sources, Nitrogen sources, pH and Temperature on the solubilization of phosphorus by A. terreus was also tested. The strain showed maximum solubilization in the presence of fructose as carbon source, ammonium sulphate as a nitrogen source, at the temperature of 30ËšC and at the pH of 7.0 in both qualitative and quantitative assays. Thus A. terreus shall be used as a viable Bioinoculant to eradicate P- deficiency in plants.
INFLUENCE OF SOIL FUNGI ON CORROSION OF MILD STEEL PLATES
(NACE - International Corrosion Conference Series, 2018-04-15) Dharani, R; Deepalakshmi, R; Padma Devi, S N; Nithya Meenakshi, S; Nalini, D
Metal corrosion is an electrochemical reaction between the environment and a metal, in which microbes are thought to play a very important role. These microorganisms do not only cause corrosion, but they can also inhibit or protect against corrosion. Fungi are the most dessicant – resistant microorganisms and are ubiquitous in atmospheric environments. About five fungal organisms were isolated using Starkey media from the soil of corroded pipeline tank. The influence of these fungal isolates on both rusted and non – rusted mild steel plates were studied for a period of 25 days. Among the five fungal isolates, Non – rusted Isolate (NR) – 1 and Rusted Isolate (R) – 3 showed minimum corrosion reaction on mild steel plates, based on the results of weight loss and dissolved iron content. The results revealed that the two isolates showed minimum rate of corrosion on mild steel plates due to the passive mechanism of microbes upon the plates. Therefore the above isolates (NR -1 and R- 3) was identified using molecular markers and it was found to be Aspergillus flavus and Alternaria alternata respectively.
ISOLATION AND IDENTIFICATION OF ZINC SOLUBILIZING FUNGAL ISOLATES FROMAGRICULTURAL FIELDS
(Indian Journal of Agricultural Sciences, 2015-12) Anitha, S; Padma Devi, S N; Sunitha Kumari, K
ORGANIC REMOBILIZATION OF ZINC AND PHOSPHORUS AVAILABILITY TO PLANTS BY APPLICATION OF MINERAL SOLUBILIZING BACTERIA PSEUDOMONAS AERUGINOSA
(Elsevier Ltd, 2023-11) Sunitha Kumari, K; Padma Devi, S N; Rajamani, Ranjithkumar; Sinouvassane, Djearamane; Lai-Hock, Tey; Ling Shing, Wong; Saminathan, Kayarohanam; Natarajan, Arumugam; Abdulrahman I, Almansour; Karthikeyan, Perumal
Incessant utilization of chemical fertilizers leads to the accumulation of minerals in the soil, rendering them unavailable to plants. Unaware of the mineral reserves present in the soil, farming communities employ chemical fertilizers once during each cultivation, a practice that causes elevated levels of insoluble minerals within the soil. The use of biofertilizers on the other hand, reduces the impact of chemical fertilizers through the action of microorganisms in the product, which dissolves minerals and makes them readily available for plant uptake, helping to create a sustainable environment for continuous agricultural production. In the current investigation, a field trial employing Arachis hypogaea L was conducted to evaluate the ability of Pseudomonas aeruginosa to enhance plant growth and development by solubilizing minerals present in the soil (such as zinc and phosphorus). A Randomized Complete Block Design (RCBD) included five different treatments as T1: Un inoculated Control; T2: Seeds treated with a liquid formulation of P. aeruginosa; T3: Seeds treated with a liquid formulation of P. aeruginosa and the soil amended with organic manure (farmyard); T4: Soil amended with organic manure (farmyard) alone; T5: Seeds treated with lignite (solid) based formulation of P. aeruginosa were used for the study. Efficacy was determined based on the plant's morphological characters and mineral contents (Zn and P) of plants and soil. Survival of P. aeruginosa in the field was validated using Antibiotic Intrinsic patterns (AIP). The results indicated that the combination treatment of P. aeruginosa liquid formulation and organic fertilizer (farmyard) (T3) produced the highest biometric parameters and mineral (Zn and P) content of the groundnut plants and the soil. This outcome is likely attributed to the mineral solubilizing capability of P. aeruginosa. Furthermore, the presence of farmyard manure increased the metabolic activity of P. aeruginosa by inducing its heterotrophic activity, leading to higher mineral content in T3 soil compared to other soil treatments. The AIP data confirmed the presence of the applied liquid inoculant by exhibiting a similar intrinsic pattern between the in vitro isolate and the isolate obtained from the fields. In summary, the Zn and P solubilization ability of P. aeruginosa facilitates the conversion of soil-unavailable mineral form into a form accessible to plants. It further proposes the utilization of the liquid formulation of P. aeruginosa as a viable solution to mitigate the challenges linked to solid-based biofertilizers and the reliance on mineral-based chemical fertilizers.
ORGANIC REMOBILIZATION OF ZINC AND PHOSPHORUS AVAILABILITY TO PLANTS BY APPLICATION OF MINERAL SOLUBILIZING BACTERIA PSEUDOMONAS AERUGINOSA
(Elsevier Ltd, 2023-11) Sunitha Kumari, K; Padma Devi, S N; Rajamani, RanjithKumar; Natarajan, Arumugam; Abdulrahman I, Almansour; Karthikeyan, Perumal
Incessant utilization of chemical fertilizers leads to the accumulation of minerals in the soil, rendering them unavailable to plants. Unaware of the mineral reserves present in the soil, farming communities employ chemical fertilizers once during each cultivation, a practice that causes elevated levels of insoluble minerals within the soil. The use of biofertilizers on the other hand, reduces the impact of chemical fertilizers through the action of microorganisms in the product, which dissolves minerals and makes them readily available for plant uptake, helping to create a sustainable environment for continuous agricultural production. In the current investigation, a field trial employing Arachis hypogaea L was conducted to evaluate the ability of Pseudomonas aeruginosa to enhance plant growth and development by solubilizing minerals present in the soil (such as zinc and phosphorus). A Randomized Complete Block Design (RCBD) included five different treatments as T1: Un inoculated Control; T2: Seeds treated with a liquid formulation of P. aeruginosa; T3: Seeds treated with a liquid formulation of P. aeruginosa and the soil amended with organic manure (farmyard); T4: Soil amended with organic manure (farmyard) alone; T5: Seeds treated with lignite (solid) based formulation of P. aeruginosa were used for the study. Efficacy was determined based on the plant's morphological characters and mineral contents (Zn and P) of plants and soil. Survival of P. aeruginosa in the field was validated using Antibiotic Intrinsic patterns (AIP). The results indicated that the combination treatment of P. aeruginosa liquid formulation and organic fertilizer (farmyard) (T3) produced the highest biometric parameters and mineral (Zn and P) content of the groundnut plants and the soil. This outcome is likely attributed to the mineral solubilizing capability of P. aeruginosa. Furthermore, the presence of farmyard manure increased the metabolic activity of P. aeruginosa by inducing its heterotrophic activity, leading to higher mineral content in T3 soil compared to other soil treatments. The AIP data confirmed the presence of the applied liquid inoculant by exhibiting a similar intrinsic pattern between the in vitro isolate and the isolate obtained from the fields. In summary, the Zn and P solubilization ability of P. aeruginosa facilitates the conversion of soil-unavailable mineral form into a form accessible to plants. It further proposes the utilization of the liquid formulation of P. aeruginosa as a viable solution to mitigate the challenges linked to solid-based biofertilizers and the reliance on mineral-based chemical fertilizers.
ZINC SOLUBILIZING BACTERIAL ISOLATES FROM THE AGRICULTURAL FIELDS OF COIMBATORE, TAMIL NADU, INDIA
(Current Science, 2016-01) Sunithakumari, K; Padma Devi, S N; Vasandha, S
Zinc plays a pivotal role in physiological and biochemical functions of the plants. Both quantitative and qualitative yield of the plants are strongly dependent on this micronutrient. Supplementation of zinc in the form of synthetic fertilizer is proved to be inappropriate due to its unavailability to plants. This crisis can be prevented by the identification of rhizospheric micro-organisms which has the potential to transform various unavailable forms of the metal to available forms. In the present study about thirty five zinc solubilizing bacteria were isolated from eight different agricultural fields (banana, chilli, field bean, ground nut, maize, sugarcane, sorghum and tomato) in and around Coimbatore district of Tamil Nadu. Five isolates were selected as best strains based on their solubilization efficacy in the qualitative estimation. The selected five isolates were identified using 16S rRNA as Stenotrophomonas maltophilia (ZSB-1), Mycobacterium brisbanense (ZSB-10), Enterobacter aerogenes (ZSB-13), Pseudomonas aeruginosa (ZSB22) and Xanthomonas retroflexus (ZSB-23). These strains were subjected to further studies such as quantitative estimation, influence of the isolates on the pH of the medium and production of gluconic acid as well as IAA. Of the five bacterial isolates, Pseudomonas aeruginosa showed maximum solubilization of zinc in the broth and also maximum decrease in the pH from 7 to 3.3 and recorded highest IAA production. HPLC analysis of gluconic acid production by the selected isolates indicated their potential to solubilize zinc.