Browsing by Author "P, Muthukumaran"

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    MOLECULAR CHARACTERIZATION OF PAPAYA RING SPOT VIRUS ISOLATES FROM DIFFERENT PARTS OF TAMIL NADU
    (2021-03-02) Nirmal Kumar, Ramasamy; V.G, Shobana; R, Kannan; P, Muthukumaran; K, Angappan
    An attempt has been made to characterize different PRSV isolates which infects papaya plants were collected from different parts of Tamil Nadu. The isolates were of PRSV-P type strain and the Coat Protein gene (CP) varied in size from 840- 860 bp which encodes 280 - 284 amino acids. Sequence alignment results revealed that the seven sequences obtained from different regions shared a homology of about 90% -95% with each other and homology of about 96% with the already reported Indian isolate and 88-90% with the other country isolates collected from NCBI. KE repeats were observed in the N terminus of the CP coding region from the different isolates found out. Phylogenetic analysis revealed that the isolates identified here placed on the same group and reference isolates were grouped in a different progeny. Our study further helped us to identify conserved regions among the seven isolates and we also constructed an RNAi silencing vector targeting Coat protein gene to challenge the Papaya Ring Spot Virus (PRSV) for our future study
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    PHYTOCHEMICAL CHARACTERIZATION OF BIOACTIVE COMPOUND FROM THE ENSETE SUPERBUM SEED POWDER
    (2018-12-27) R, Nirmal Kumar; P, Muthukumaran; K, Suresh Kumar; R, Karthikeyen
    The bioactive components Ensete superbum Seed Powder has been evaluated using GCMS, HPLC, FTIR and HNMR. The chemical compositions of the extract of Ensete superbum Seed Powder was investigated by Gas Chromatography–Mass Spectrometry and GC/MS techniques. The analysis of extract of Ensete superbum Seed Powder revealed that the existence of Eugenol (39.51) n-Hexadecanoic acid (21.97), 9-Eicosyne (5.18), 3-Decanynoic acid(1.87), 1- Tetradecyne(5.30), 7-Methyl-Z-tetradecen-1-ol acetate(1.88), 1-Hexadecyne (9.71), Z-(13,14- Epoxy)tetradec-11-en-1-ol acetate(2.61), Octadecanoic acid (6.01), Tridecanedial (4.78) and cis-13-Eicosenoic acid (1.17). HPLC analysis of Ensete superbum Seed Powder reported that it has mainly contains five flavonoids compounds, namely Gallic acid (5.550 min), Caffeic acid (9.233min), Rutin (10.317min), Quercetin (12.125min) and Ferulic acid (23.200min). The results of FTIR analysis confirmed the presence of Alkynes, Alkanes , Amines , aromatic amines, alkyl halides, alkenes, carboxylic acids and Aromatic Compounds. Further, proton nuclear magnetic resonance spectrum of Ensete superbum Seed Powder was recorded and the chemical shift values of the various signals are identified. The detection of these phytochemical compounds present in the medicinal plants will endow with some information on Ensete superbum Seed Powder as herbal alternative for cure vast array of diseases.