Browsing by Author "Nayana, Chathalingath"

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BIOSYNTHESIS AND BIODEGRADATION OF POLY(3-HYDROXYBUTYRATE) FROM PRIESTIA FLEXA; A PROMISING MANGROVE HALOPHYTE TOWARDS THE DEVELOPMENT OF SUSTAINABLE ECO-FRIENDLY BIOPLASTICS
(Elsevier, 2023-02) Nayana, Chathalingath; Joshua, Stephen Kingsly; Anbarasi, Gunasekar
The protracted persistence of petrochemical plastics in the environment and their non-biodegradability impede the survival of living creatures. Recently, biopolymers are being thoroughly researched as a potential replacement for conventional plastics. This present study sought to locate Poly(3-hydroxybutyrate) synthesizing bacterial species prevalent in the mangrove ecosystem. Six halophilic bacterial isolates were obtained from the mangrove habitat, four isolates displayed superior cell dry weight as well as PHB accumulation. Isolate PMPHB5 showed the highest cell dry weight (4.92 ± 0.02 g/L), while the maximum PHA yield (80%) was found with PMPHB7. Hence, PMPHB7 was chosen for further optimization of carbon source wherein glucose demonstrated improved cell growth as well as PHB production. The characterization of the PHB granules was performed by FT-IR spectroscopy and FE-SEM EDX. The presence of characteristic elements in the sample was confirmed using EDX. Isolate PMPHB7 was further identified as Priestia flexa through 16S rRNA gene sequencing (GenBank accession number: ON362236) and a phylogenetic tree was constructed to reveal the molecular relationships of this organism with others. The solvent-cast biopolymer film was made to check the biodegradability of the extracted PHB. When buried in soil, it was found that the biopolymer film exhibited approximately 73% biodegradation after 21 days. Thus, the present study sheds light on the potential of mangrove-associated halophytes to efficiently produce PHB that is readily biodegradable in soil.
BIOSYNTHESIS AND BIODEGRADATION OF POLY(3-HYDROXYBUTYRATE) FROM PRIESTIA FLEXA; A PROMISING MANGROVE HALOPHYTE TOWARDS THE DEVELOPMENT OF SUSTAINABLE ECO-FRIENDLY BIOPLASTICS
(Elsevier GmbH, 2023-02) Nayana, Chathalingath; Joshua, Stephen Kingsly; Anbarasi, Gunasekar
The protracted persistence of petrochemical plastics in the environment and their non-biodegradability impede the survival of living creatures. Recently, biopolymers are being thoroughly researched as a potential replacement for conventional plastics. This present study sought to locate Poly(3-hydroxybutyrate) synthesizing bacterial species prevalent in the mangrove ecosystem. Six halophilic bacterial isolates were obtained from the mangrove habitat, four isolates displayed superior cell dry weight as well as PHB accumulation. Isolate PMPHB5 showed the highest cell dry weight (4.92 ± 0.02 g/L), while the maximum PHA yield (80%) was found with PMPHB7. Hence, PMPHB7 was chosen for further optimization of carbon source wherein glucose demonstrated improved cell growth as well as PHB production. The characterization of the PHB granules was performed by FT-IR spectroscopy and FE-SEM EDX. The presence of characteristic elements in the sample was confirmed using EDX. Isolate PMPHB7 was further identified as Priestia flexa through 16S rRNA gene sequencing (GenBank accession number: ON362236) and a phylogenetic tree was constructed to reveal the molecular relationships of this organism with others. The solvent-cast biopolymer film was made to check the biodegradability of the extracted PHB. When buried in soil, it was found that the biopolymer film exhibited approximately 73% biodegradation after 21 days. Thus, the present study sheds light on the potential of mangrove-associated halophytes to efficiently produce PHB that is readily biodegradable in soil.
BIOSYNTHESIS AND BIODEGRADATION OF POLY(3-HYDROXYBUTYRATE) FROM PRIESTIA FLEXA; A PROMISING MANGROVE HALOPHYTE TOWARDS THE DEVELOPMENT OF SUSTAINABLE ECO-FRIENDLY BIOPLASTICS
(Elsevier, 2023-02) Nayana, Chathalingath; Joshua, Stephen Kingsly; Anbarasi, Gunasekar
The protracted persistence of petrochemical plastics in the environment and their non-biodegradability impede the survival of living creatures. Recently, biopolymers are being thoroughly researched as a potential replacement for conventional plastics. This present study sought to locate Poly(3-hydroxybutyrate) synthesizing bacterial species prevalent in the mangrove ecosystem. Six halophilic bacterial isolates were obtained from the mangrove habitat, four isolates displayed superior cell dry weight as well as PHB accumulation. Isolate PMPHB5 showed the highest cell dry weight (4.92 ± 0.02 g/L), while the maximum PHA yield (80%) was found with PMPHB7. Hence, PMPHB7 was chosen for further optimization of carbon source wherein glucose demonstrated improved cell growth as well as PHB production. The characterization of the PHB granules was performed by FT-IR spectroscopy and FE-SEM EDX. The presence of characteristic elements in the sample was confirmed using EDX. Isolate PMPHB7 was further identified as Priestia flexa through 16S rRNA gene sequencing (GenBank accession number: ON362236) and a phylogenetic tree was constructed to reveal the molecular relationships of this organism with others. The solvent-cast biopolymer film was made to check the biodegradability of the extracted PHB. When buried in soil, it was found that the biopolymer film exhibited approximately 73% biodegradation after 21 days. Thus, the present study sheds light on the potential of mangrove-associated halophytes to efficiently produce PHB that is readily biodegradable in soil.
ELUCIDATING THE PHYSIOLOGICAL AND MOLECULAR CHARACTERISTICS OF BACTERIAL BLIGHT INCITANT XANTHOMONAS AUXONOPODIS PV. PUNICAE; A LIFE THREATENING PHYTOPATHOGEN OF POMEGRANATE (PUNICA GRANATUM. L) AND ASSESSMENT OF H2O2 ACCUMULATION DURING HOST-PATHOGEN INTERACTION
(Elsevier Ltd, 2023-09) Nayana, Chathalingath; Anbarasi, Gunasekar
Bacterial blight of pomegranate caused by Xanthomonas auxonopodis pv.punicae (Xap) threaten the existence of a group of farmers for the past few decades who rely on pomegranate cultivation for their livelihood since it will cause huge yield loss. The primary focus of this study was to conduct a thorough analysis of the characterization of this blight incitant Xap. Physiological, biochemical, and molecular characteristics of six phytopathogenic strains of Xap, designated as PBF1 (PBF: Pomegranate Blight Fruit), PBF2, PBF3, PBF4, PBF5, and PBF6, isolated from the infected fruits were examined. Bacterial colonies were featured as gram-negative, yellow-pigmented circular with a glistening appearance. An attempt to determine the best culture medium, favouring bacterial proliferation was successfully done with four distinct medium, Nutrient Glucose Agar (NGA), Nutrient sucrose Agar (NSA), Yeast Dextrose Calcium Carbonate Agar (YDCA) and Yeast Glucose Calcium Carbonate Agar (YGCA) and comparatively, significant growth was found in NGA (66.66%) followed by YDCA (33%). According to the antibiotic susceptibility results, both ampicillin and streptomycin were determined as potentially effective drugs in preventing the proliferation of Xap (P 0.05). The reactive oxygen species-mediated plant immune response during host-pathogen interaction was confirmed by accessing the presence of H2O2 accumulation in infected leaves via 3,3 - diaminobenzidine (DAB) -staining technique. Bacterial isolates from this study were confirmed by two universal constitutive genes such as gyrB and 16S rRNA. From the BLAST analysis, the isolates were identified as Xap with base pair lengths of 1408bp, 1180bp, and 1159bp, which correspond to PBF1, PBF2, and PBF3, respectively. A neighbor-joining phylogenetic tree study explaining a strong phylogenetic relationship between the query sequence and closely related bacterial species.
ELUCIDATING THE PHYSIOLOGICAL AND MOLECULAR CHARACTERISTICS OF BACTERIAL BLIGHT INCITANT XANTHOMONAS AUXONOPODIS PV. PUNICAE; A LIFE THREATENING PHYTOPATHOGEN OF POMEGRANATE (PUNICA GRANATUM. L) AND ASSESSMENT OF H2O2 ACCUMULATION DURING HOST-PATHOGEN INTERACTION
(Elsevier, 2023-09) Nayana, Chathalingath; Anbarasi, Gunasekar
Bacterial blight of pomegranate caused by Xanthomonas auxonopodis pv. punicae (Xap) threaten the existence of a group of farmers for the past few decades who rely on pomegranate cultivation for their livelihood since it will cause huge yield loss. The primary focus of this study was to conduct a thorough analysis of the characterization of this blight incitant Xap. Physiological, biochemical, and molecular characteristics of six phytopathogenic strains of Xap, designated as PBF1 (PBF: Pomegranate Blight Fruit), PBF2, PBF3, PBF4, PBF5, and PBF6, isolated from the infected fruits were examined. Bacterial colonies were featured as gram-negative, yellow-pigmented circular with a glistening appearance. An attempt to determine the best culture medium, favouring bacterial proliferation was successfully done with four distinct medium, Nutrient Glucose Agar (NGA), Nutrient sucrose Agar (NSA), Yeast Dextrose Calcium Carbonate Agar (YDCA) and Yeast Glucose Calcium Carbonate Agar (YGCA) and comparatively, significant growth was found in NGA (66.66%) followed by YDCA (33%). According to the antibiotic susceptibility results, both ampicillin and streptomycin were determined as potentially effective drugs in preventing the proliferation of Xap (P 0.05). The reactive oxygen species-mediated plant immune response during host-pathogen interaction was confirmed by accessing the presence of H2O2 accumulation in infected leaves via 3,3 - diaminobenzidine (DAB) -staining technique. Bacterial isolates from this study were confirmed by two universal constitutive genes such as gyrB and 16S rRNA. From the BLAST analysis, the isolates were identified as Xap with base pair lengths of 1408bp, 1180bp, and 1159bp, which correspond to PBF1, PBF2, and PBF3, respectively. A neighbor-joining phylogenetic tree study explaining a strong phylogenetic relationship between the query sequence and closely related bacterial species.
ELUCIDATING THE PHYSIOLOGICAL AND MOLECULAR CHARACTERISTICS OF BACTERIAL BLIGHT INCITANT XANTHOMONAS AUXONOPODIS PV. PUNICAE; A LIFE THREATENING PHYTOPATHOGEN OF POMEGRANATE (PUNICA GRANATUM. L) AND ASSESSMENT OF H2O2 ACCUMULATION DURING HOST-PATHOGEN INTERACTION
(Pub Med, 2023-07-28) Nayana, Chathalingath; Anbarasi, Gunasekar
Bacterial blight of pomegranate caused by Xanthomonas auxonopodis pv.punicae (Xap) threaten the existence of a group of farmers for the past few decades who rely on pomegranate cultivation for their livelihood since it will cause huge yield loss. The primary focus of this study was to conduct a thorough analysis of the characterization of this blight incitant Xap. Physiological, biochemical, and molecular characteristics of six phytopathogenic strains of Xap, designated as PBF1 (PBF: Pomegranate Blight Fruit), PBF2, PBF3, PBF4, PBF5, and PBF6, isolated from the infected fruits were examined. Bacterial colonies were featured as gram-negative, yellow-pigmented circular with a glistening appearance. An attempt to determine the best culture medium, favouring bacterial proliferation was successfully done with four distinct medium, Nutrient Glucose Agar (NGA), Nutrient sucrose Agar (NSA), Yeast Dextrose Calcium Carbonate Agar (YDCA) and Yeast Glucose Calcium Carbonate Agar (YGCA) and comparatively, significant growth was found in NGA (66.66%) followed by YDCA (33%). According to the antibiotic susceptibility results, both ampicillin and streptomycin were determined as potentially effective drugs in preventing the proliferation of Xap (P 0.05). The reactive oxygen species-mediated plant immune response during host-pathogen interaction was confirmed by accessing the presence of H2O2 accumulation in infected leaves via 3,3 - diaminobenzidine (DAB) -staining technique. Bacterial isolates from this study were confirmed by two universal constitutive genes such as gyrB and 16S rRNA. From the BLAST analysis, the isolates were identified as Xap with base pair lengths of 1408bp, 1180bp, and 1159bp, which correspond to PBF1, PBF2, and PBF3, respectively. A neighbor-joining phylogenetic tree study explaining a strong phylogenetic relationship between the query sequence and closely related bacterial species.
PHENOTYPIC AND MOLECULAR CHARACTERISATION OF XANTHOMONAS AXONOPODIS PV. PUNICAE FROM POMEGRANATE LEAVES
(Elsevier, 2023-11) Nayana, Chathalingath; Anbarasi, Gunasekar; Sreelakshmy, Venu
The present study has sought to provide insight into the blight-inciting phytopathogen Xanthomonas axonopodis pv. punicae, causing a devasting outbreak in pomegranate cultivation. Yellow-coloured circular with glistening featured colonies of five isolates of X. axonopodis pv. punicae have been phenotypically characterized and the identity of the isolates was confirmed by specific gyrB and 16S rRNA gene sequences. Results of BLAST analysis of PBL1 and PBL2 showed 99.64% and 99.76% identity with X. axonopodis pv. punicae strain 1(GenBank: KY354098.1) and strain 15 IARI (GenBank: JN036628.1) respectively. Further phylogenetic analysis revealed the strong association of X. axonopodis pv. punicae isolates with other Xanthomonas pathovars such as X. phaseoli pv. manihotis, X. citri pv. punicae and X. citri pv. mangiferaeindicae. An attempt was made to find the optimal growth media for bacterial proliferation and it was evident that NGA served as an excellent medium to support the multiplication of each isolate with a significant number of colonies (P < 0.05). The medium order per the best CFU/ml was NGA > YDCA > YGCA > NSA. The drug susceptibility test gained a lot of attention since most plant pathogens acquired resistance to antibiotics. Both streptomycin and amoxicillin could act as potentially effective drugs in inhibiting the growth of X. axonopodis pv. punicae (P 0.05). The H2O2 accumulation during the pathogen attack in the infected leaves was located by an endogenous peroxidase-dependent in situ histochemical staining method. Brown precipitate visible at a different colour intensity demonstrated the efficiency of PAMPs to trigger plant immunity to produce H2O2. The present study was the first report on the isolation and characterization of X. axonopodis pv. punicae isolated from blight-infected Pomegranate leaves from the mentioned regions of Tamil Nadu, India.