Browsing by Author "Monisha, Sivanandhan"

Now showing 1 - 10 of 10

FACILE APPROACH FOR GREEN SYNTHESIS OF FLUORESCENT CARBON DOTS FROM MANIHOT ESCULENTA AND THEIR POTENTIAL APPLICATIONS AS SENSOR AND BIO-IMAGING AGENTS
(Elsevier, 2022-03) Monisha, Sivanandhan; Amutha, Parasuraman; Chinnasamy, Surya; Komalavalli, Lakshminarayanan; Balu, Krishnakumar; Durai, Mani; Young-Ho, Ahn
An eco-friendly approach for the synthesis of blue fluorescent carbon dots (CDs) using Manihot esculenta with an average size of 3–5 nm through hydrothermal method has been reported. Functionality of the prepared CDs was characterized by FT-IR spectroscopy. Structural and morphological characterisation were carried with XRD and TEM techniques, these studies exposed amorphous nature of carbons dots with spherical shape. Optical properties of CDs were examined using UV–Visible and Photoluminescence spectral techniques. Owing to strong blue fluorescence, CDs were evaluated for its ability to sense various heavy metal ions. Attributable to significant response towards Fe3+ ions among different metal ions tested (Al3+, Cu+, Co2+, Pb2+, Zn2+, Fe3+, Ca2+, Hg2+ and Mg2+), CDs were used as a selective fluorescence probe for detection of Fe3+. The bioimaging and cytotoxicity aspects of CDs on MDA MB-231 breast cancer cell lines were confirmed by localised experiments. The synthesised CDs were found to be non-toxic even at their high concentration of 500 µg/mL.
FACILE APPROACH FOR GREEN SYNTHESIS OF FLUORESCENT CARBON DOTS FROM MANIHOT ESCULENTA AND THEIR POTENTIAL APPLICATIONS AS SENSOR AND BIO-IMAGING AGENTS
(Elsevier, 2022-03) Monisha, Sivanandhan; Amutha, Parasuraman; Chinnasamy, Surya; Komalavalli, Lakshminarayanan; Balu, Krishnakumar; Durai, Mani; Young-Ho, Ahn
An eco-friendly approach for the synthesis of blue fluorescent carbon dots (CDs) using Manihot esculenta with an average size of 3–5 nm through hydrothermal method has been reported. Functionality of the prepared CDs was characterized by FT-IR spectroscopy. Structural and morphological characterisation were carried with XRD and TEM techniques, these studies exposed amorphous nature of carbons dots with spherical shape. Optical properties of CDs were examined using UV–Visible and Photoluminescence spectral techniques. Owing to strong blue fluorescence, CDs were evaluated for its ability to sense various heavy metal ions. Attributable to significant response towards Fe3+ ions among different metal ions tested (Al3+, Cu+, Co2+, Pb2+, Zn2+, Fe3+, Ca2+, Hg2+ and Mg2+), CDs were used as a selective fluorescence probe for detection of Fe3+. The bioimaging and cytotoxicity aspects of CDs on MDA MB-231 breast cancer cell lines were confirmed by localised experiments. The synthesised CDs were found to be non-toxic even at their high concentration of 500 µg/mL.
IN-SILICO MOLECULAR DOCKING AND ADMET PREDICTIONS OF PYRIDO[2,3-D]PYRIMIDINE-2,4(1H,3H)-DIONE ANALOGUES AS PROMISING ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER AGENTS
(2024) Monisha, Sivanandhan; Amutha, Parasuraman
Pyridopyrimidine are heterocyclic molecules enclosing fused pyridine and pyrimidine rings. Owing to its fascinating core structure and pharmacological applications a series of 7-([1,1′-biphenyl]-4-yl)-5-arylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-diones were synthesized and characterized using IR, 1H, 13C NMR and Mass spectral techniques. The antibacterial, antioxidant and anticancer activities were investigated for the synthesized compound 5a-5f. Compounds with electron-donating groups showed excellent free radical scavenging activity. Halogen-substituted compounds showed more potent antimicrobial and anticancer activity than other derivatives in comparison with their respective standards. Based on the IC50 value obtained from anticancer activity, 5c was further analyzed for apoptosis by AO/EB staining method. The findings suggested early apoptosis in the MCF-7 cell line. Molecular Docking studies of the synthesized compounds were performed with Kinase 1 inhibitors (PDB id: 2YEX), 5c exposed good docking results with minimum binding energy. Further, these compounds were acknowledged as orally active drug candidates from in-silico ADMET studies. Computational analysis supports biological findings indicating compound 5c as a promising anticancer agent against the human breast cancer MCF-7 cell line.
IN-SILICO MOLECULAR DOCKING AND ADMET PREDICTIONS OF PYRIDO[2,3-D]PYRIMIDINE-2,4(1H,3H)-DIONE ANALOGUES AS PROMISING ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER AGENTS
(Taylor and Francis Ltd., 2024) Monisha, Sivanandhan; Amutha, Parasuraman
Pyridopyrimidine are heterocyclic molecules enclosing fused pyridine and pyrimidine rings. Owing to its fascinating core structure and pharmacological applications a series of 7-([1,1′-biphenyl]-4-yl)-5-arylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-diones were synthesized and characterized using IR, 1H, 13C NMR and Mass spectral techniques. The antibacterial, antioxidant and anticancer activities were investigated for the synthesized compound 5a-5f. Compounds with electron-donating groups showed excellent free radical scavenging activity. Halogen-substituted compounds showed more potent antimicrobial and anticancer activity than other derivatives in comparison with their respective standards. Based on the IC50 value obtained from anticancer activity, 5c was further analyzed for apoptosis by AO/EB staining method. The findings suggested early apoptosis in the MCF-7 cell line. Molecular Docking studies of the synthesized compounds were performed with Kinase 1 inhibitors (PDB id: 2YEX), 5c exposed good docking results with minimum binding energy. Further, these compounds were acknowledged as orally active drug candidates from in-silico ADMET studies. Computational analysis supports biological findings indicating compound 5c as a promising anticancer agent against the human breast cancer MCF-7 cell line.
IN-SILICO MOLECULAR DOCKING AND ADMET PREDICTIONS OF PYRIDO[2,3-D]PYRIMIDINE-2,4(1H,3H)-DIONE ANALOGUES AS PROMISING ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER AGENTS
(Taylor and Francis Ltd, 2024-04-02) Monisha, Sivanandhan; Amutha, Parasuraman
Pyridopyrimidine are heterocyclic molecules enclosing fused pyridine and pyrimidine rings. Owing to its fascinating core structure and pharmacological applications a series of 7-([1,1′-biphenyl]-4-yl)-5-arylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-diones were synthesized and characterized using IR, 1H, 13C NMR and Mass spectral techniques. The antibacterial, antioxidant and anticancer activities were investigated for the synthesized compound 5a-5f. Compounds with electron-donating groups showed excellent free radical scavenging activity. Halogen-substituted compounds showed more potent antimicrobial and anticancer activity than other derivatives in comparison with their respective standards. Based on the IC50 value obtained from anticancer activity, 5c was further analyzed for apoptosis by AO/EB staining method. The findings suggested early apoptosis in the MCF-7 cell line. Molecular Docking studies of the synthesized compounds were performed with Kinase 1 inhibitors (PDB id: 2YEX), 5c exposed good docking results with minimum binding energy. Further, these compounds were acknowledged as orally active drug candidates from in-silico ADMET studies. Computational analysis supports biological findings indicating compound 5c as a promising anticancer agent against the human breast cancer MCF-7 cell line.
IN-SILICO MOLECULAR DOCKING AND ADMET PREDICTIONS OF PYRIDO[2,3-D]PYRIMIDINE-2,4(1H,3H)-DIONE ANALOGUES AS PROMISING ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER AGENTS
(Elsevier, 2023-06-09) Monisha, Sivanandhan; Amutha, Parasuraman
Pyridopyrimidine are heterocyclic molecules enclosing fused pyridine and pyrimidine rings. Owing to its fascinating core structure and pharmacological applications a series of 7-([1,1′-biphenyl]-4-yl)-5-arylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-diones were synthesized and characterized using IR, 1H, 13C NMR and Mass spectral techniques. The antibacterial, antioxidant and anticancer activities were investigated for the synthesized compound 5a-5f. Compounds with electron-donating groups showed excellent free radical scavenging activity. Halogen-substituted compounds showed more potent antimicrobial and anticancer activity than other derivatives in comparison with their respective standards. Based on the IC50 value obtained from anticancer activity, 5c was further analyzed for apoptosis by AO/EB staining method. The findings suggested early apoptosis in the MCF-7 cell line. Molecular Docking studies of the synthesized compounds were performed with Kinase 1 inhibitors (PDB id: 2YEX), 5c exposed good docking results with minimum binding energy. Further, these compounds were acknowledged as orally active drug candidates from in-silico ADMET studies. Computational analysis supports biological findings indicating compound 5c as a promising anticancer agent against the human breast cancer MCF-7 cell line.
IN-SILICO MOLECULAR DOCKING AND ADMET PREDICTIONS OF PYRIDO[2,3-D]PYRIMIDINE-2,4(1H,3H)-DIONE ANALOGUES AS PROMISING ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER AGENTS
(Taylor & Francis Online, 2023-04-02) Monisha, Sivanandhan; Amutha, Parasuraman
Pyridopyrimidine are heterocyclic molecules enclosing fused pyridine and pyrimidine rings. Owing to its fascinating core structure and pharmacological applications a series of 7-([1,1′-biphenyl]-4-yl)-5-arylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-diones were synthesized and characterized using IR, 1H, 13C NMR and Mass spectral techniques. The antibacterial, antioxidant and anticancer activities were investigated for the synthesized compound 5a-5f. Compounds with electron-donating groups showed excellent free radical scavenging activity. Halogen-substituted compounds showed more potent antimicrobial and anticancer activity than other derivatives in comparison with their respective standards. Based on the IC50 value obtained from anticancer activity, 5c was further analyzed for apoptosis by AO/EB staining method. The findings suggested early apoptosis in the MCF-7 cell line. Molecular Docking studies of the synthesized compounds were performed with Kinase 1 inhibitors (PDB id: 2YEX), 5c exposed good docking results with minimum binding energy. Further, these compounds were acknowledged as orally active drug candidates from in-silico ADMET studies. Computational analysis supports biological findings indicating compound 5c as a promising anticancer agent against the human breast cancer MCF-7 cell line.
NBCL5 FUNCTIONALIZED PERLITE: A POTENT AND RECYCLABLE CATALYST FOR SYNTHESIS OF PYRANS
(MDPI, 2023-02-16) Komalavalli, Lakshminarayanan; Monisha, Sivanandhan; Subramaniyan, Ramasundaram; Tae Hwan, Oh; Kinjal J, Shah; Kumaravel, Saranraj; Amutha, Parasuraman; Krishnakumar, Balu
Niobium pentachloride functionalised perlite was prepared via a solid state dispersion technique, which was utilized as an efficient heterogeneous catalyst for the synthesis of pyrans. The immobilisation of NbCl5 over perlite was examined by Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), scanning electron microscope (SEM) with energy dispersive spectra (EDS), and Brunauer, Emmett and Teller (BET) surface area measurements. The wt% of NbCl5-loaded perlite was optimized based on the adequacy with respect to the yield of the pyrans in various solvents. The recyclability of the catalyst was validated in synthesizing pyrans and the results marked its efficiency up to five runs. The efficacy of the NbCl5/perlite catalyst was found to be comparable and better with respect to the other heterogeneous catalysts reported. The structures of pyrans were confirmed by FT-IR, 1H and 13C NMR spectral techniques. The proposed recyclable heterogeneous NbCl5/perlite catalyst simplifies the protocol, and has minimal chemical waste, a lower reaction time and a high-yield.
NBCL5 FUNCTIONALIZED PERLITE: A POTENT AND RECYCLABLE CATALYST FOR SYNTHESIS OF PYRANS
(Sustainability (Switzerland), 2023-02-16) Komalavalli, Lakshminarayanan; Monisha, Sivanandhan; Subramaniyan, Ramasundaram; Tae, Hwan Oh; J, Kinjal Shah; Kumaravel, Saranraj; Amutha, Parasuraman; Krishnakumar, Balu
Niobium pentachloride functionalised perlite was prepared via a solid state dispersion technique, which was utilized as an efficient heterogeneous catalyst for the synthesis of pyrans. The immobilisation of NbCl5 over perlite was examined by Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), scanning electron microscope (SEM) with energy dispersive spectra (EDS), and Brunauer, Emmett and Teller (BET) surface area measurements. The wt% of NbCl5-loaded perlite was optimized based on the adequacy with respect to the yield of the pyrans in various solvents. The recyclability of the catalyst was validated in synthesizing pyrans and the results marked its efficiency up to five runs. The efficacy of the NbCl5/perlite catalyst was found to be comparable and better with respect to the other heterogeneous catalysts reported. The structures of pyrans were confirmed by FT-IR, 1H and 13C NMR spectral techniques. The proposed recyclable heterogeneous NbCl5/perlite catalyst simplifies the protocol, and has minimal chemical waste, a lower reaction time and a high-yield.
SYNTHESIS, CRYSTAL STRUCTURE, HIRSHFELD SURFACE, COMPUTATIONAL AND BIOLOGICAL STUDIES OF SPIRO-OXINDOLE DERIVATIVES AS MDM2-P53 INHIBITORS
(Springer Link, 2024-08) Monisha, Sivanandhan; Sutha, Ragupathy; Arumugam, Thangamani; Amutha, Parasuraman
The spiro-oxindole derivatives were synthesized via a 1,3-dipolar cycloaddition approach and characterized by FT-IR, 1H, 13C NMR and mass spectral techniques. The single crystal XRD of 6d further validates the formation of compounds. DFT calculations indicated the reactive nature of compound 6d. Docking studies with 5LAW disclosed the minimum binding energy of - 10.83 kcal/mol for 6d. Furthermore, safe oral bioavailability was ensured by the physicochemical, pharmacokinetic, and toxicity predictions. The anticancer analysis of synthesized compounds showed substantial activity against A549 cells, notably with an IC50 value of 8.13 ± 0.66 µM for 6d compared to standard doxorubicin. 6d was also evaluated for cytotoxicity against L929 healthy cells and A549, showing selectivity towards A549 than healthy cells. AO/EB staining method showed early apoptotic cellular death in the A549 cell line in a dose-dependent manner.