Browsing by Author "Anbarasi G"

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BIOSYNTHESIS AND CHARACTERIZATION OF CHITOSAN BASED HYDROGEL: A POTENTIAL IN VITRO WOUND HEALING AGENT
(Science Direct, 2022) Arulmoorthy M.P; Anbarasi G; Srinivasan M; Vishnupriya B
Humans are continuously plagued by number of new uncured diseases, resulting in the search of new medicines with zero level side effects. In the present study, an attempt has been made to prepare, characterize and to test the wound healing activity of chitosan based hydrogel. Shrimp shells was collected from Cuddalore (Lat 11° 42́ N Long 79° 46́ E), Annankovil (Lat. 11°30.47 N; Long. 079°47. 02E) and Mudasalodai landing centre (11o29 ′N 79o46 ′E) (Southeast coast of India) for extraction of chitin and chitosan and further characterized by FTIR, 1H – NMR, DSC, SEM and XRD. Poly Ethylene Glycol (PEG) is used as a cross linking agent for the preparation of chitosan hydrogel. The prepared hydrogel was characterized by FTIR, SEM, DSC and its swelling property was tested. Synthesized chitosan hydrogel was shown very good level of growth inhibition activity against human pathogens and wound healing activity within 12 h to 24 h. Thus the present study suggests the possibility of utilizing seafood waste of crustacean shells for the extraction and preparation of useful biologically active compounds like hydrogel for medicinal drug delivery and therapeutic applications.
BIOSYNTHESIS OF ZINC OXIDE NANOPARTICLES USING HYLOCEREUS UNDATUS FRUIT PEEL EXTRACT AGAINST CLINICAL PATHOGENS
(Science Direct, 2022-01-07) Vishnupriya B; Elakkiya Nandhini G.R; Anbarasi G
Biosynthesis of zinc oxide nanoparticles (ZnO NPs) using the peel extract of Hylocereus undatus (dragon fruit) was carried out for the first time by a green chemistry approach. The peel used for this study, act as a reducing and capping agent for synthesizing ZnO NPs from the precursor salt. The synthesized nanoparticles were characterized by UV–Visible Spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive analysis of X-ray (EDAX) and Fourier transform infrared spectroscopy (FT-IR). The anti-microbial activity of ZnO NPs were assessed against the microbial pathogens and compared with the crude extract of H. undatus. The synthesized nanoparticles showed more effective against gram positive (Bacillus subtilis) bacteria than gram negative (Escherichia coli, Klebsella Pneumoniae and Pseudomonas aeruginosa), and moderate activity was found against C. albicans. It indicates the biomedical capability of ZnO NPs.
GROWTH REGULATION AND PROTEOMIC APPROACHES OF EXOGENOUS ABSCISIC ACID INDUCED CHANGES ON SALT TOLERANCE FACTORS IN SUAEDA MARITIMA
(Plant Physiology Reports, 2020) Anbarasi G; Somasundaram S.T
Salinity is one of the most imperative global problems that affect the crop productivity on a large scale. Salinity impairs plant growth and development by imposing various stresses and it is vital to decode those stress factors and to identify possible solutions to improve agriculture productivity. It is essential to study those plants with known resistance to increased salinity in order to produce commercial crops which can survive on salt stress conditions. There are several plant species that possess exceptional physiological uniqueness which critically influences environmental adaptation. However, the adaptive mechanisms under saline conditions of halophytes have not yet been clearly distinguished. In this backdrop, the present study was undertaken to determine the effects of exogenous application of abscisic acid on salinity tolerance in Suaeda maritima. The observed data from the present study confirms that the abscisic acid pretreatment has enhanced the plant biomass, water relations and ion content. There was also a stimulated increase in the content of chlorophyll pigment and inorganic osmolytes accumulation. Comparative proteomic analysis was performed to investigate the changes in protein profiles of both the treated and untreated plants. Among the expressed proteins, 5.25 PI/48 MW spot exhibited twofold decrease in NaCl treatment and sixfold increases in ABA pretreated plants. The protein spot had a match with a significant score of 94% in the closely related SKP1-like protein 1A (Sesamum indicum). Hence, the present study supports ABA-mediated stress response is co-regulated by SKP1.
KINETIC STUDIES OF PARTIALLY PURIFIED LIPASE FROM MARINE ACTINOMYCETE STREPTOMYCES ACRIMYCINI NGP 1
(Kongunadu Arts and Science College, 2020) Vishnupriya B; Anbarasi G
This study was focused on partial purification and characterization of lipase from Streptomyces acrimyciniNGP 1, isolated from marine sediment of south Indian coastal region. In purification steps, 4.53 fold purification was achieved after 85% ammonium sulphate precipitation with 0.97 percent recovery. In further purification steps, 1.33 fold purification was achieved by Sephadex G-100 chromatography with 1.61 percent of recovery. The specific activity of purified enzyme was 1525 U/mg. Zymogram of crude enzyme on native-PAGE presented bands with lipase activity of molecular weight and Isoelectric point were 50 kDa and 7.4 respectively. These features render this lipase of interest as a biocatalyst for applications such as biodiesel production and detergent formulations.